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Light-induced linear dichroism in photoreversibly photochromic sensor pigments 2. Chromophore rotation in immobilized phytochrome

Sundqvist, C and Björn, Lars Olof LU (1983) In Photochemistry and Photobiology 37(1). p.69-75
Abstract
Large phytochrome ((P) from Avena sativa) immobilized via anti-phytochrome immunoglobulin bound to Sepharose beads was irradiated to saturation with unpolarized far-red (fr) light. The apparent absorbance level was recorded in a dual wavelength spectrophotometer with both measuring beams set to either 600 or 730 nm and polarized perpendicular to each other. The sample was then irradiated with red (r) polarized light. The apparent change in absorbance obtained after this irradiation indicated that purified phytochrome could show linear dichroism. From the absorbance values obtained it was computed that the direction of the long-wavelength transition moment changes by either 32.degree. or 148.degree., when phytochrome is transformed from P1r... (More)
Large phytochrome ((P) from Avena sativa) immobilized via anti-phytochrome immunoglobulin bound to Sepharose beads was irradiated to saturation with unpolarized far-red (fr) light. The apparent absorbance level was recorded in a dual wavelength spectrophotometer with both measuring beams set to either 600 or 730 nm and polarized perpendicular to each other. The sample was then irradiated with red (r) polarized light. The apparent change in absorbance obtained after this irradiation indicated that purified phytochrome could show linear dichroism. From the absorbance values obtained it was computed that the direction of the long-wavelength transition moment changes by either 32.degree. or 148.degree., when phytochrome is transformed from P1r to Pfr. Considering the model of Hahn and Song (1981), the latter value appears more likely. In light of these results, the conclusions drawn from in vivo experiments on action dichroism in Dryopteris, Adiantum (Kadota et al.,1982) and Mougeoutia, which point to a 90.degree. rotation should be reconsidered. (Less)
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organization
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Contribution to journal
publication status
published
subject
in
Photochemistry and Photobiology
volume
37
issue
1
pages
69 - 75
publisher
American Society for Photobiology
external identifiers
  • scopus:84989688323
ISSN
0031-8655
language
English
LU publication?
yes
id
51b4e1c1-8c66-4ead-bd33-333a1c7b9d8c (old id 134617)
date added to LUP
2007-07-30 11:13:11
date last changed
2017-08-06 03:38:39
@article{51b4e1c1-8c66-4ead-bd33-333a1c7b9d8c,
  abstract     = {Large phytochrome ((P) from Avena sativa) immobilized via anti-phytochrome immunoglobulin bound to Sepharose beads was irradiated to saturation with unpolarized far-red (fr) light. The apparent absorbance level was recorded in a dual wavelength spectrophotometer with both measuring beams set to either 600 or 730 nm and polarized perpendicular to each other. The sample was then irradiated with red (r) polarized light. The apparent change in absorbance obtained after this irradiation indicated that purified phytochrome could show linear dichroism. From the absorbance values obtained it was computed that the direction of the long-wavelength transition moment changes by either 32.degree. or 148.degree., when phytochrome is transformed from P1r to Pfr. Considering the model of Hahn and Song (1981), the latter value appears more likely. In light of these results, the conclusions drawn from in vivo experiments on action dichroism in Dryopteris, Adiantum (Kadota et al.,1982) and Mougeoutia, which point to a 90.degree. rotation should be reconsidered.},
  author       = {Sundqvist, C and Björn, Lars Olof},
  issn         = {0031-8655},
  language     = {eng},
  number       = {1},
  pages        = {69--75},
  publisher    = {American Society for Photobiology},
  series       = {Photochemistry and Photobiology},
  title        = {Light-induced linear dichroism in photoreversibly photochromic sensor pigments 2. Chromophore rotation in immobilized phytochrome},
  volume       = {37},
  year         = {1983},
}