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Transcriptional responses of Paxillus involutus and Betula pendula during formation of ectomycorrhizal root tissue

Johansson, Tomas LU ; Le Quéré, Antoine LU ; Ahrén, Dag LU ; Söderström, Bengt LU ; Erlandsson, Rickard; Lundeberg, Joakim; Uhlen, Mattias and Tunlid, Anders LU (2004) In Molecular Plant-Microbe Interactions 17(2). p.202-215
Abstract
In order to obtain information on genes specifically expressed in the ectomycorrhizal symbiosis, 3,555 expressed sequence tags (ESTs) were analyzed from a cDNA library constructed from ectomycorrhiza formed between the basidiomycete Paxillus involutus and birch (Betula pendula). cDNA libraries from saprophytically growing fungus (3,964 ESTs) and from axenic plants (2,532 ESTs) were analyzed in parallel. By clustering all the EST obtained, a nonredundant set of 2,284 unique transcripts of either fungal or plant origin were identified. The expression pattern of these genes was analyzed using cDNA microarrays. The analyses showed that the plant and fungus responded to the symbiosis by altering the expression levels of a number of enzymes... (More)
In order to obtain information on genes specifically expressed in the ectomycorrhizal symbiosis, 3,555 expressed sequence tags (ESTs) were analyzed from a cDNA library constructed from ectomycorrhiza formed between the basidiomycete Paxillus involutus and birch (Betula pendula). cDNA libraries from saprophytically growing fungus (3,964 ESTs) and from axenic plants (2,532 ESTs) were analyzed in parallel. By clustering all the EST obtained, a nonredundant set of 2,284 unique transcripts of either fungal or plant origin were identified. The expression pattern of these genes was analyzed using cDNA microarrays. The analyses showed that the plant and fungus responded to the symbiosis by altering the expression levels of a number of enzymes involved in carbon metabolism. Several plant transcripts with sequence similarities to genes encoding enzymes in the tricarboxylic cycle and electron transport chain were down regulated as compared with the levels in free-living roots. In the fungal partner, a number of genes encoding enzymes in the lipid and secondary metabolism were down regulated in mycorrhiza as compared with the saprophytically growing mycelium. A substantial number of the ESTs analyzed displayed significant sequence similarities to proteins involved in biotic stress responses, but only a few of them showed differential expression in the mycorrhizal tissue, including plant and fungal metallothioneins and a plant defensin homologue. Several of the genes that were differentially expressed in the mycorrhizal root tissue displayed sequence similarity to genes that are known to regulate growth and development of plant roots and fungal hyphae, including transcription factors and Rho-like GTPases. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Plant-Microbe Interactions
volume
17
issue
2
pages
202 - 215
publisher
American Physical Society
external identifiers
  • wos:000220329400008
  • pmid:14964534
  • scopus:1642345273
ISSN
0894-0282
DOI
10.1094/MPMI.2004.17.2.202
language
English
LU publication?
yes
id
1cf9b273-ea02-4a49-aff4-ee9ca1e34cc8 (old id 135660)
date added to LUP
2007-06-29 11:12:49
date last changed
2017-11-12 03:19:06
@article{1cf9b273-ea02-4a49-aff4-ee9ca1e34cc8,
  abstract     = {In order to obtain information on genes specifically expressed in the ectomycorrhizal symbiosis, 3,555 expressed sequence tags (ESTs) were analyzed from a cDNA library constructed from ectomycorrhiza formed between the basidiomycete Paxillus involutus and birch (Betula pendula). cDNA libraries from saprophytically growing fungus (3,964 ESTs) and from axenic plants (2,532 ESTs) were analyzed in parallel. By clustering all the EST obtained, a nonredundant set of 2,284 unique transcripts of either fungal or plant origin were identified. The expression pattern of these genes was analyzed using cDNA microarrays. The analyses showed that the plant and fungus responded to the symbiosis by altering the expression levels of a number of enzymes involved in carbon metabolism. Several plant transcripts with sequence similarities to genes encoding enzymes in the tricarboxylic cycle and electron transport chain were down regulated as compared with the levels in free-living roots. In the fungal partner, a number of genes encoding enzymes in the lipid and secondary metabolism were down regulated in mycorrhiza as compared with the saprophytically growing mycelium. A substantial number of the ESTs analyzed displayed significant sequence similarities to proteins involved in biotic stress responses, but only a few of them showed differential expression in the mycorrhizal tissue, including plant and fungal metallothioneins and a plant defensin homologue. Several of the genes that were differentially expressed in the mycorrhizal root tissue displayed sequence similarity to genes that are known to regulate growth and development of plant roots and fungal hyphae, including transcription factors and Rho-like GTPases.},
  author       = {Johansson, Tomas and Le Quéré, Antoine and Ahrén, Dag and Söderström, Bengt and Erlandsson, Rickard and Lundeberg, Joakim and Uhlen, Mattias and Tunlid, Anders},
  issn         = {0894-0282},
  language     = {eng},
  number       = {2},
  pages        = {202--215},
  publisher    = {American Physical Society},
  series       = {Molecular Plant-Microbe Interactions},
  title        = {Transcriptional responses of Paxillus involutus and Betula pendula during formation of ectomycorrhizal root tissue},
  url          = {http://dx.doi.org/10.1094/MPMI.2004.17.2.202},
  volume       = {17},
  year         = {2004},
}