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The microbial community in the rhizosphere determined by community-level physiological profiles (CLPP) and direct soil- and cfu-PLFA techniques

Söderberg, Katarina LU ; Probanza, A; Jumpponen, A and Bååth, Erland LU (2004) In Agriculture, Ecosystems & Environment. Applied Soil Ecology 25(2). p.135-145
Abstract
The composition of the microbial community in the rhizosphere was investigated using three different techniques: direct soil-phospholipid fatty acid (PLFA), cfu-PLFA (using culturable, bacteria only) and community-level physiological profiles (CLPP) using Biolog GN microtitre plates. All three techniques showed that the rhizosphere bacterial community differed from the bulk soil community, when pea plants were grown in pots. The two PLFA techniques also indicated that the effect of roots on microbial communities was greater than differences in the PLFA pattern between different bulk soils. The difference was most prominent using the direct soil-PLFA technique. Both PLFA techniques indicated that gram-negative bacteria were relatively more... (More)
The composition of the microbial community in the rhizosphere was investigated using three different techniques: direct soil-phospholipid fatty acid (PLFA), cfu-PLFA (using culturable, bacteria only) and community-level physiological profiles (CLPP) using Biolog GN microtitre plates. All three techniques showed that the rhizosphere bacterial community differed from the bulk soil community, when pea plants were grown in pots. The two PLFA techniques also indicated that the effect of roots on microbial communities was greater than differences in the PLFA pattern between different bulk soils. The difference was most prominent using the direct soil-PLFA technique. Both PLFA techniques indicated that gram-negative bacteria were relatively more abundant in the rhizosphere, while PLFAs indicative of gram-positive bacteria were relatively more common in bulk soil samples. The results obtained with the direct soil-PLFA method did not differ between rhizosphere samples taken along a pea root, while this was the case with both the cfu-PLFA technique and the CUP method. According to both techniques, the differences in community composition were greatest near the root tip. Different plant species exhibited different microbial community composition in the rhizosphere, as judged from all three methods, when four different plant species were compared. In all cases, Trifolium differed most from the other species (three different grasses). Although all three methods were found to be suitable for rhizosphere studies, the CLPP method appeared less suitable than the two PLFA methods, since less of the variation in the data could usually be explained. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Agriculture, Ecosystems & Environment. Applied Soil Ecology
volume
25
issue
2
pages
135 - 145
publisher
Elsevier
external identifiers
  • wos:000189004200004
  • scopus:1642564156
ISSN
0929-1393
DOI
10.1016/j.apsoil.2003.08.005
language
English
LU publication?
yes
id
e78cc31b-b351-4271-93fc-48fde1980b7f (old id 135679)
date added to LUP
2007-06-29 14:46:43
date last changed
2017-12-10 03:40:31
@article{e78cc31b-b351-4271-93fc-48fde1980b7f,
  abstract     = {The composition of the microbial community in the rhizosphere was investigated using three different techniques: direct soil-phospholipid fatty acid (PLFA), cfu-PLFA (using culturable, bacteria only) and community-level physiological profiles (CLPP) using Biolog GN microtitre plates. All three techniques showed that the rhizosphere bacterial community differed from the bulk soil community, when pea plants were grown in pots. The two PLFA techniques also indicated that the effect of roots on microbial communities was greater than differences in the PLFA pattern between different bulk soils. The difference was most prominent using the direct soil-PLFA technique. Both PLFA techniques indicated that gram-negative bacteria were relatively more abundant in the rhizosphere, while PLFAs indicative of gram-positive bacteria were relatively more common in bulk soil samples. The results obtained with the direct soil-PLFA method did not differ between rhizosphere samples taken along a pea root, while this was the case with both the cfu-PLFA technique and the CUP method. According to both techniques, the differences in community composition were greatest near the root tip. Different plant species exhibited different microbial community composition in the rhizosphere, as judged from all three methods, when four different plant species were compared. In all cases, Trifolium differed most from the other species (three different grasses). Although all three methods were found to be suitable for rhizosphere studies, the CLPP method appeared less suitable than the two PLFA methods, since less of the variation in the data could usually be explained.},
  author       = {Söderberg, Katarina and Probanza, A and Jumpponen, A and Bååth, Erland},
  issn         = {0929-1393},
  language     = {eng},
  number       = {2},
  pages        = {135--145},
  publisher    = {Elsevier},
  series       = {Agriculture, Ecosystems & Environment. Applied Soil Ecology},
  title        = {The microbial community in the rhizosphere determined by community-level physiological profiles (CLPP) and direct soil- and cfu-PLFA techniques},
  url          = {http://dx.doi.org/10.1016/j.apsoil.2003.08.005},
  volume       = {25},
  year         = {2004},
}