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The influence of nitrogen fertilisation on bacterial activity in the rhizosphere of barley

Söderberg, Katarina LU and Bååth, Erland LU (2004) In Soil Biology & Biochemistry 36(1). p.195-198
Abstract
The effect of nitrogen addition on the activity of rhizosphere bacteria was studied using barley seedlings. Three different nitrogen sources were added to the soil (nitrate, ammonium and ammonium + nitrate) at four different concentrations (0, 100, 300 and 500 mg N kg(-1) soil) and the plants were allowed to grow for 6 weeks. The bacterial activity was estimated by measuring thymidine and leucine incorporation into bacteria extracted using homogenisation-centrifugation. Bulk soil bacterial activity was low compared with that of rhizosphere bacteria. Nitrogen addition did not affect the activity of the bulk soil bacteria, indicating that the activity was not nitrogen limited. The thymidine and leucine incorporation rates of rhizosphere... (More)
The effect of nitrogen addition on the activity of rhizosphere bacteria was studied using barley seedlings. Three different nitrogen sources were added to the soil (nitrate, ammonium and ammonium + nitrate) at four different concentrations (0, 100, 300 and 500 mg N kg(-1) soil) and the plants were allowed to grow for 6 weeks. The bacterial activity was estimated by measuring thymidine and leucine incorporation into bacteria extracted using homogenisation-centrifugation. Bulk soil bacterial activity was low compared with that of rhizosphere bacteria. Nitrogen addition did not affect the activity of the bulk soil bacteria, indicating that the activity was not nitrogen limited. The thymidine and leucine incorporation rates of rhizosphere bacteria decreased when ammonium or ammonium + nitrate was applied compared with the non-amended controls. No effect on bacterial activity was found following nitrate addition. There was a significant positive correlation between rhizosphere bacterial activity and rhizosphere pH. Shoot length following ammonium treatment was significant lower than in the non-amended control, while nitrate and ammonium + nitrate addition had no effect. This indicates that the varying effects due to nitrogen sources on rhizosphere bacterial activity were not due to effects on plant growth. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soil Biology & Biochemistry
volume
36
issue
1
pages
195 - 198
publisher
Elsevier
external identifiers
  • wos:000188587600022
  • scopus:0842332280
ISSN
0038-0717
DOI
10.1016/j.soilbio.2003.07.001
language
English
LU publication?
yes
id
f4c716c7-9749-4a89-b985-4dd019ac9e9b (old id 135689)
date added to LUP
2007-06-29 14:47:55
date last changed
2017-01-01 06:38:36
@article{f4c716c7-9749-4a89-b985-4dd019ac9e9b,
  abstract     = {The effect of nitrogen addition on the activity of rhizosphere bacteria was studied using barley seedlings. Three different nitrogen sources were added to the soil (nitrate, ammonium and ammonium + nitrate) at four different concentrations (0, 100, 300 and 500 mg N kg(-1) soil) and the plants were allowed to grow for 6 weeks. The bacterial activity was estimated by measuring thymidine and leucine incorporation into bacteria extracted using homogenisation-centrifugation. Bulk soil bacterial activity was low compared with that of rhizosphere bacteria. Nitrogen addition did not affect the activity of the bulk soil bacteria, indicating that the activity was not nitrogen limited. The thymidine and leucine incorporation rates of rhizosphere bacteria decreased when ammonium or ammonium + nitrate was applied compared with the non-amended controls. No effect on bacterial activity was found following nitrate addition. There was a significant positive correlation between rhizosphere bacterial activity and rhizosphere pH. Shoot length following ammonium treatment was significant lower than in the non-amended control, while nitrate and ammonium + nitrate addition had no effect. This indicates that the varying effects due to nitrogen sources on rhizosphere bacterial activity were not due to effects on plant growth.},
  author       = {Söderberg, Katarina and Bååth, Erland},
  issn         = {0038-0717},
  language     = {eng},
  number       = {1},
  pages        = {195--198},
  publisher    = {Elsevier},
  series       = {Soil Biology & Biochemistry},
  title        = {The influence of nitrogen fertilisation on bacterial activity in the rhizosphere of barley},
  url          = {http://dx.doi.org/10.1016/j.soilbio.2003.07.001},
  volume       = {36},
  year         = {2004},
}