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Elemental composition of ectomycorrhizal mycelia identified by PCR-RFLP analysis and grown in contact with apatite or wood ash in forest soil

Wallander, Håkan LU ; Mahmood, Shahid LU ; Hagerberg, David LU ; Johansson, Leif LU and Pallon, Jan LU (2003) In FEMS Microbiology Ecology 44(1). p.57-65
Abstract
The aim of this study was to identify ectomycorrhizal species with a potential to release elements from apatite and wood ash and accumulate them in the mycelia. Fungal rhizomorphs and mycelia were sampled from sand-filled mesh bags with or without amendment of apatite or wood ash. The mesh bags were buried in forest soil in the field for 13 or 24 months. Elemental composition of the samples was analyzed with particle-induced X-ray emission and the fungus was identified by polymerase chain reaction-restriction fragment length polymorphism analysis of the ITS-region of ribosomal DNA. The majority of rhizomorphs and mycelia collected from the mesh bags were of mycorrhizal origin with Paxillus involutus being the most common species (31%).... (More)
The aim of this study was to identify ectomycorrhizal species with a potential to release elements from apatite and wood ash and accumulate them in the mycelia. Fungal rhizomorphs and mycelia were sampled from sand-filled mesh bags with or without amendment of apatite or wood ash. The mesh bags were buried in forest soil in the field for 13 or 24 months. Elemental composition of the samples was analyzed with particle-induced X-ray emission and the fungus was identified by polymerase chain reaction-restriction fragment length polymorphism analysis of the ITS-region of ribosomal DNA. The majority of rhizomorphs and mycelia collected from the mesh bags were of mycorrhizal origin with Paxillus involutus being the most common species (31%). Other identified species were Thelephora terrestris, Suillus granulatus and Tylospora fibillosa. S. granulatus contained 3-15 times more K (3 mg g(-1)) than the other species and had large calcium-rich crystals deposited on the surface of rhizomorphs when grown in contact with apatite. P. involutus contained the largest amount of Ca (2-7 mg g(-1)). Wood ash addition increased the amount of Ti, Mn and Pb in the rhizomorphs while apatite addition increased the amount of Ca in the rhizomorphs. The high concentration of K in S. granulatus rhizomorphs suggests that this fungus is a good accumulator of K while P. involutus appeared to accumulate heavy metals originating from wood ash. (Less)
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organization
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Contribution to journal
publication status
published
subject
in
FEMS Microbiology Ecology
volume
44
issue
1
pages
57 - 65
publisher
Elsevier
external identifiers
  • wos:000182357700007
  • scopus:0041881874
ISSN
1574-6941
DOI
language
English
LU publication?
yes
id
9fb99062-e738-458d-8d09-9feac6c7d3ff (old id 135759)
date added to LUP
2007-06-29 15:47:10
date last changed
2018-05-29 10:35:19
@article{9fb99062-e738-458d-8d09-9feac6c7d3ff,
  abstract     = {The aim of this study was to identify ectomycorrhizal species with a potential to release elements from apatite and wood ash and accumulate them in the mycelia. Fungal rhizomorphs and mycelia were sampled from sand-filled mesh bags with or without amendment of apatite or wood ash. The mesh bags were buried in forest soil in the field for 13 or 24 months. Elemental composition of the samples was analyzed with particle-induced X-ray emission and the fungus was identified by polymerase chain reaction-restriction fragment length polymorphism analysis of the ITS-region of ribosomal DNA. The majority of rhizomorphs and mycelia collected from the mesh bags were of mycorrhizal origin with Paxillus involutus being the most common species (31%). Other identified species were Thelephora terrestris, Suillus granulatus and Tylospora fibillosa. S. granulatus contained 3-15 times more K (3 mg g(-1)) than the other species and had large calcium-rich crystals deposited on the surface of rhizomorphs when grown in contact with apatite. P. involutus contained the largest amount of Ca (2-7 mg g(-1)). Wood ash addition increased the amount of Ti, Mn and Pb in the rhizomorphs while apatite addition increased the amount of Ca in the rhizomorphs. The high concentration of K in S. granulatus rhizomorphs suggests that this fungus is a good accumulator of K while P. involutus appeared to accumulate heavy metals originating from wood ash.},
  author       = {Wallander, Håkan and Mahmood, Shahid and Hagerberg, David and Johansson, Leif and Pallon, Jan},
  issn         = {1574-6941},
  language     = {eng},
  number       = {1},
  pages        = {57--65},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Ecology},
  title        = {Elemental composition of ectomycorrhizal mycelia identified by PCR-RFLP analysis and grown in contact with apatite or wood ash in forest soil},
  url          = {http://dx.doi.org/},
  volume       = {44},
  year         = {2003},
}