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The prevalence, population structure and screening test specificity of penicillin-susceptible Staphylococcus aureus bacteremia isolates in Malmö, Sweden

Resman, Fredrik LU ; Thegerström, John LU ; Månsson, Fredrik LU ; Ahl, Jonas LU ; Tham, Johan LU and Riesbeck, Kristian LU (2016) In Journal of Infection 73(2). p.129-135
Abstract

Objectives The objectives of this study were to examine the prevalence of penicillin-susceptible bacteremic Staphylococcus aureus in the Malmö area in 2014, to re-evaluate the phenotypic methods of penicillinase detection on these isolates, and to investigate the clonal distribution of penicillin-susceptible isolates. Methods All non-redundant S. aureus from blood in the Malmö catchment area in southern Sweden 2014 were screened for penicillin susceptibility using PcG 1U disk diffusion, E-test PcG and the nitrocefin test. All isolates screened as likely susceptible were subjected to PCR for detection of penicillinase (blaZ) and spa-typing. Results Almost one out of three bacteremic isolates (80/257; 31.1%) were susceptible to... (More)

Objectives The objectives of this study were to examine the prevalence of penicillin-susceptible bacteremic Staphylococcus aureus in the Malmö area in 2014, to re-evaluate the phenotypic methods of penicillinase detection on these isolates, and to investigate the clonal distribution of penicillin-susceptible isolates. Methods All non-redundant S. aureus from blood in the Malmö catchment area in southern Sweden 2014 were screened for penicillin susceptibility using PcG 1U disk diffusion, E-test PcG and the nitrocefin test. All isolates screened as likely susceptible were subjected to PCR for detection of penicillinase (blaZ) and spa-typing. Results Almost one out of three bacteremic isolates (80/257; 31.1%) were susceptible to penicillin. All screening methods except for the nitrocefin test alone had a low proportion of isolates falsely tested as susceptible, but no method used in the study had perfect specificity compared with PCR. Penicillin-susceptible isolates had a distinct phylogenetic distribution, and two clonal complexes (CC5 and CC45) constituted half of the isolates. Conclusion Almost one third of S. aureus isolated from blood in southern Sweden in 2014 was susceptible to penicillin. Considering that intravenous penicillin has theoretical advantages compared with the standard treatment in the study area, we argue that routine testing of penicillin susceptibility should be reconsidered.

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Bacteremia, Penicillin, Staphylococcus aureus, Susceptibility testing
in
Journal of Infection
volume
73
issue
2
pages
7 pages
publisher
W B Saunders
external identifiers
  • scopus:84989813272
  • wos:000383380000005
ISSN
0163-4453
DOI
10.1016/j.jinf.2016.05.011
language
English
LU publication?
yes
id
135aa7ae-022d-4394-bae6-d09f4a712195
date added to LUP
2016-12-30 09:52:38
date last changed
2017-09-18 11:34:39
@article{135aa7ae-022d-4394-bae6-d09f4a712195,
  abstract     = {<p>Objectives The objectives of this study were to examine the prevalence of penicillin-susceptible bacteremic Staphylococcus aureus in the Malmö area in 2014, to re-evaluate the phenotypic methods of penicillinase detection on these isolates, and to investigate the clonal distribution of penicillin-susceptible isolates. Methods All non-redundant S. aureus from blood in the Malmö catchment area in southern Sweden 2014 were screened for penicillin susceptibility using PcG 1U disk diffusion, E-test PcG and the nitrocefin test. All isolates screened as likely susceptible were subjected to PCR for detection of penicillinase (blaZ) and spa-typing. Results Almost one out of three bacteremic isolates (80/257; 31.1%) were susceptible to penicillin. All screening methods except for the nitrocefin test alone had a low proportion of isolates falsely tested as susceptible, but no method used in the study had perfect specificity compared with PCR. Penicillin-susceptible isolates had a distinct phylogenetic distribution, and two clonal complexes (CC5 and CC45) constituted half of the isolates. Conclusion Almost one third of S. aureus isolated from blood in southern Sweden in 2014 was susceptible to penicillin. Considering that intravenous penicillin has theoretical advantages compared with the standard treatment in the study area, we argue that routine testing of penicillin susceptibility should be reconsidered.</p>},
  author       = {Resman, Fredrik and Thegerström, John and Månsson, Fredrik and Ahl, Jonas and Tham, Johan and Riesbeck, Kristian},
  issn         = {0163-4453},
  keyword      = {Bacteremia,Penicillin,Staphylococcus aureus,Susceptibility testing},
  language     = {eng},
  month        = {08},
  number       = {2},
  pages        = {129--135},
  publisher    = {W B Saunders},
  series       = {Journal of Infection},
  title        = {The prevalence, population structure and screening test specificity of penicillin-susceptible Staphylococcus aureus bacteremia isolates in Malmö, Sweden},
  url          = {http://dx.doi.org/10.1016/j.jinf.2016.05.011},
  volume       = {73},
  year         = {2016},
}