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Phosphorus limitation of bacterial growth in high Arctic lakes and ponds

Granéli, Wilhelm LU ; Bertilsson, S and Philibert, A (2004) In Aquatic Sciences 66(4). p.430-439
Abstract
Water from lakes and tundra ponds on Banks, Melville, Ellef-Ringnes, Ellesmere and Devon Island (74 - 79 degreesN, 82 - 116 degreesW) in the Canadian high Arctic was studied in batch culture experiments to test whether nitrogen, phosphorus or organic carbon limited bacterial growth and biomass accumulation. Water samples containing indigenous bacteria were amended with carbon ( glucose), nitrogen ( nitrate) or phosphorus ( phosphate), either alone or in combination, and were incubated in the dark at ambient temperatures. Bacterial growth was measured as the rate of protein synthesis and the accumulation of bacterial cells. Bacterial growth was significantly enhanced in all cultures amended with phosphorus. There was no indication of... (More)
Water from lakes and tundra ponds on Banks, Melville, Ellef-Ringnes, Ellesmere and Devon Island (74 - 79 degreesN, 82 - 116 degreesW) in the Canadian high Arctic was studied in batch culture experiments to test whether nitrogen, phosphorus or organic carbon limited bacterial growth and biomass accumulation. Water samples containing indigenous bacteria were amended with carbon ( glucose), nitrogen ( nitrate) or phosphorus ( phosphate), either alone or in combination, and were incubated in the dark at ambient temperatures. Bacterial growth was measured as the rate of protein synthesis and the accumulation of bacterial cells. Bacterial growth was significantly enhanced in all cultures amended with phosphorus. There was no indication of primary carbon or nitrogen limitation in either lakes or ponds, but the combined addition of phosphorus and either carbon, nitrogen or both, had a positive effect on bacterial growth in the lakes but not in ponds. This contrasting response in Arctic lakes and ponds can be predicted from in situ concentrations of dissolved nutrients: total dissolved phosphorus was low in all systems (= 10 mug L-1), whereas total dissolved nitrogen and organic carbon was on average 24 and 7 times higher in ponds. Pelagic bacteria in lakes and ponds of the high Arctic seem to follow the general pattern of phosphorus limitation previously observed in many temperate and tropical freshwater systems. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Aquatic Sciences
volume
66
issue
4
pages
430 - 439
publisher
Birkhaüser
external identifiers
  • wos:000224888400010
  • scopus:9744230691
ISSN
1420-9055
DOI
10.1007/s00027-004-0732-7
language
English
LU publication?
yes
id
560eee2e-8abf-4aee-b6a7-53cf34d026aa (old id 136615)
date added to LUP
2007-06-28 13:28:20
date last changed
2017-12-10 03:46:10
@article{560eee2e-8abf-4aee-b6a7-53cf34d026aa,
  abstract     = {Water from lakes and tundra ponds on Banks, Melville, Ellef-Ringnes, Ellesmere and Devon Island (74 - 79 degreesN, 82 - 116 degreesW) in the Canadian high Arctic was studied in batch culture experiments to test whether nitrogen, phosphorus or organic carbon limited bacterial growth and biomass accumulation. Water samples containing indigenous bacteria were amended with carbon ( glucose), nitrogen ( nitrate) or phosphorus ( phosphate), either alone or in combination, and were incubated in the dark at ambient temperatures. Bacterial growth was measured as the rate of protein synthesis and the accumulation of bacterial cells. Bacterial growth was significantly enhanced in all cultures amended with phosphorus. There was no indication of primary carbon or nitrogen limitation in either lakes or ponds, but the combined addition of phosphorus and either carbon, nitrogen or both, had a positive effect on bacterial growth in the lakes but not in ponds. This contrasting response in Arctic lakes and ponds can be predicted from in situ concentrations of dissolved nutrients: total dissolved phosphorus was low in all systems (= 10 mug L-1), whereas total dissolved nitrogen and organic carbon was on average 24 and 7 times higher in ponds. Pelagic bacteria in lakes and ponds of the high Arctic seem to follow the general pattern of phosphorus limitation previously observed in many temperate and tropical freshwater systems.},
  author       = {Granéli, Wilhelm and Bertilsson, S and Philibert, A},
  issn         = {1420-9055},
  language     = {eng},
  number       = {4},
  pages        = {430--439},
  publisher    = {Birkhaüser},
  series       = {Aquatic Sciences},
  title        = {Phosphorus limitation of bacterial growth in high Arctic lakes and ponds},
  url          = {http://dx.doi.org/10.1007/s00027-004-0732-7},
  volume       = {66},
  year         = {2004},
}