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Mediator facilitated, laccase catalysed oxidation of granular potato starch and the physico-chemical characterisation of the oxidized products.

Mathew, Sindhu and Adlercreutz, Patrick LU (2009) In Bioresource Technology 100. p.3576-3584
Abstract
The primary hydroxyl groups in potato starch were selectively oxidized to the corresponding aldehyde and carboxylic acid functionalities by mediators like TEMPO, using laccase from fungi as catalytic oxidant and oxygen as the primary oxidant. Oxidized starch products with degree of substitution (DS(CHO) ranging from 0.16 to 16.4/100AGU and DS(COOH) from 0.01 to 3.71 carboxyl groups/100AGU) were obtained with mediator facilitated enzymatic oxidation. Maximum conversion of the primary alcohol group was obtained at a pH of 5, with TEMPO as mediator, under oxygen bubbling and two step administration of Trametes versicolor laccase (200+200nkat/g of starch). The oxidized products were characterised by IR spectroscopy, XRD and thermal studies. In... (More)
The primary hydroxyl groups in potato starch were selectively oxidized to the corresponding aldehyde and carboxylic acid functionalities by mediators like TEMPO, using laccase from fungi as catalytic oxidant and oxygen as the primary oxidant. Oxidized starch products with degree of substitution (DS(CHO) ranging from 0.16 to 16.4/100AGU and DS(COOH) from 0.01 to 3.71 carboxyl groups/100AGU) were obtained with mediator facilitated enzymatic oxidation. Maximum conversion of the primary alcohol group was obtained at a pH of 5, with TEMPO as mediator, under oxygen bubbling and two step administration of Trametes versicolor laccase (200+200nkat/g of starch). The oxidized products were characterised by IR spectroscopy, XRD and thermal studies. In the oxidized samples, the larger starch granules exhibited cracks and fractures in comparison to the smaller granules which were relatively unaffected, as observed from the microstructural studies. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Bioresource Technology
volume
100
pages
3576 - 3584
publisher
Elsevier
external identifiers
  • wos:000266153700020
  • pmid:19299125
  • scopus:64849085583
ISSN
1873-2976
DOI
10.1016/j.biortech.2009.02.035
language
English
LU publication?
yes
id
93a971b0-c1ed-4f54-bddf-2a16f327a0a8 (old id 1367644)
date added to LUP
2009-04-07 08:01:29
date last changed
2017-03-05 03:41:26
@article{93a971b0-c1ed-4f54-bddf-2a16f327a0a8,
  abstract     = {The primary hydroxyl groups in potato starch were selectively oxidized to the corresponding aldehyde and carboxylic acid functionalities by mediators like TEMPO, using laccase from fungi as catalytic oxidant and oxygen as the primary oxidant. Oxidized starch products with degree of substitution (DS(CHO) ranging from 0.16 to 16.4/100AGU and DS(COOH) from 0.01 to 3.71 carboxyl groups/100AGU) were obtained with mediator facilitated enzymatic oxidation. Maximum conversion of the primary alcohol group was obtained at a pH of 5, with TEMPO as mediator, under oxygen bubbling and two step administration of Trametes versicolor laccase (200+200nkat/g of starch). The oxidized products were characterised by IR spectroscopy, XRD and thermal studies. In the oxidized samples, the larger starch granules exhibited cracks and fractures in comparison to the smaller granules which were relatively unaffected, as observed from the microstructural studies.},
  author       = {Mathew, Sindhu and Adlercreutz, Patrick},
  issn         = {1873-2976},
  language     = {eng},
  pages        = {3576--3584},
  publisher    = {Elsevier},
  series       = {Bioresource Technology},
  title        = {Mediator facilitated, laccase catalysed oxidation of granular potato starch and the physico-chemical characterisation of the oxidized products.},
  url          = {http://dx.doi.org/10.1016/j.biortech.2009.02.035},
  volume       = {100},
  year         = {2009},
}