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A new nested polymerase chain reaction method very efficient in detecting Plasmodium and Haemoproteus infections from avian blood

Waldenström, Jonas LU ; Bensch, Staffan LU ; Hasselquist, Dennis LU and Östman, Ö (2004) In Journal of Parasitology 90(1). p.191-194
Abstract
Recently, several polymerase chain reaction (PCR)-based methods for detection and genetic identification of haemosporidian parasites in avian blood have been developed. Most of these have considerably higher sensitivity compared with traditional microscope-based examinations of blood smears. These new methods have already had a strong impact on several aspects of research on avian blood parasites. In this study. We present a new nested PCR approach, building on a previously published PCR method, which has significantly improved performance. We compare the new method with some existing assays and show, by sequence-based data, that the higher detection rate is mainly due to superior detection of Plasmodium spp. infections, which often are of... (More)
Recently, several polymerase chain reaction (PCR)-based methods for detection and genetic identification of haemosporidian parasites in avian blood have been developed. Most of these have considerably higher sensitivity compared with traditional microscope-based examinations of blood smears. These new methods have already had a strong impact on several aspects of research on avian blood parasites. In this study. We present a new nested PCR approach, building on a previously published PCR method, which has significantly improved performance. We compare the new method with some existing assays and show, by sequence-based data, that the higher detection rate is mainly due to superior detection of Plasmodium spp. infections, which often are of low intensity and, therefore, hard to detect with other methods. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Parasitology
volume
90
issue
1
pages
191 - 194
publisher
American Society of Parasitologists
external identifiers
  • wos:000220367300035
  • pmid:15040694
  • scopus:1442274864
ISSN
0022-3395
language
English
LU publication?
yes
id
93047ae0-82d2-4675-930e-23c33c635279 (old id 137056)
alternative location
http://www.bioone.org/bioone/?request=get-abstract&issn=0022-3395&volume=090&issue=01&page=0191
date added to LUP
2007-06-25 14:06:02
date last changed
2017-12-10 04:35:00
@article{93047ae0-82d2-4675-930e-23c33c635279,
  abstract     = {Recently, several polymerase chain reaction (PCR)-based methods for detection and genetic identification of haemosporidian parasites in avian blood have been developed. Most of these have considerably higher sensitivity compared with traditional microscope-based examinations of blood smears. These new methods have already had a strong impact on several aspects of research on avian blood parasites. In this study. We present a new nested PCR approach, building on a previously published PCR method, which has significantly improved performance. We compare the new method with some existing assays and show, by sequence-based data, that the higher detection rate is mainly due to superior detection of Plasmodium spp. infections, which often are of low intensity and, therefore, hard to detect with other methods.},
  author       = {Waldenström, Jonas and Bensch, Staffan and Hasselquist, Dennis and Östman, Ö},
  issn         = {0022-3395},
  language     = {eng},
  number       = {1},
  pages        = {191--194},
  publisher    = {American Society of Parasitologists},
  series       = {Journal of Parasitology},
  title        = {A new nested polymerase chain reaction method very efficient in detecting Plasmodium and Haemoproteus infections from avian blood},
  volume       = {90},
  year         = {2004},
}