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Buffer medium exchange in continuous cell and particle streams using ultrasonic standing wave focusing

Augustsson, Per LU ; Åberg, Lena B; Sward-Nilsson, Ann-Margret K. and Laurell, Thomas LU (2009) In Mikrochimica Acta 164(3-4). p.269-277
Abstract
A microfluidic strategy to perform buffer exchange of particle and cell suspensions in a continuous flow format on, chip is presented. Ultrasonic standing wave technology is utilized to confine particulate matter to the centre of a buffer exchange channel while particle free buffer is sequentially aspirated via capillaries that branch off from the buffer exchange channel. At each such branch, clean buffer is supplied at an equal flow-rate from a capillary at the opposing channel wall, generating a sideways translation of the original buffer, laminated with a wash buffer stream. Each such junction increases the buffer exchange ratio accordingly. The reported buffer exchange system provides means to adjust buffer exchange conditions on-line... (More)
A microfluidic strategy to perform buffer exchange of particle and cell suspensions in a continuous flow format on, chip is presented. Ultrasonic standing wave technology is utilized to confine particulate matter to the centre of a buffer exchange channel while particle free buffer is sequentially aspirated via capillaries that branch off from the buffer exchange channel. At each such branch, clean buffer is supplied at an equal flow-rate from a capillary at the opposing channel wall, generating a sideways translation of the original buffer, laminated with a wash buffer stream. Each such junction increases the buffer exchange ratio accordingly. The reported buffer exchange system provides means to adjust buffer exchange conditions on-line by tuning the ratio of the cross-flow wash buffer relative the sample suspension flow, rate. The system performance was evaluated using 5 mu m polystyrene microbeads and a dye as the model contaminant. Wash efficiencies up to 96.4% were accomplished with a 0.2% solid content bead suspension, using eight cross-flow junctions, effectively exchanging the carrier buffer twice. The corresponding data for erythrocyte washing was recorded to be 98.3% at a haematocrit of 2%. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Buffer medium exchange, radiation force, Acoustic, Erythrocytes, Ultrasonic standing wave, Particle washing
in
Mikrochimica Acta
volume
164
issue
3-4
pages
269 - 277
publisher
Springer
external identifiers
  • wos:000263381600006
  • scopus:60349103436
ISSN
1436-5073
DOI
10.1007/s00604-008-0084-4
language
English
LU publication?
yes
id
37d88605-2477-4343-a708-9fed0ed3df55 (old id 1372129)
date added to LUP
2009-05-08 12:10:40
date last changed
2017-09-24 03:38:42
@article{37d88605-2477-4343-a708-9fed0ed3df55,
  abstract     = {A microfluidic strategy to perform buffer exchange of particle and cell suspensions in a continuous flow format on, chip is presented. Ultrasonic standing wave technology is utilized to confine particulate matter to the centre of a buffer exchange channel while particle free buffer is sequentially aspirated via capillaries that branch off from the buffer exchange channel. At each such branch, clean buffer is supplied at an equal flow-rate from a capillary at the opposing channel wall, generating a sideways translation of the original buffer, laminated with a wash buffer stream. Each such junction increases the buffer exchange ratio accordingly. The reported buffer exchange system provides means to adjust buffer exchange conditions on-line by tuning the ratio of the cross-flow wash buffer relative the sample suspension flow, rate. The system performance was evaluated using 5 mu m polystyrene microbeads and a dye as the model contaminant. Wash efficiencies up to 96.4% were accomplished with a 0.2% solid content bead suspension, using eight cross-flow junctions, effectively exchanging the carrier buffer twice. The corresponding data for erythrocyte washing was recorded to be 98.3% at a haematocrit of 2%.},
  author       = {Augustsson, Per and Åberg, Lena B and Sward-Nilsson, Ann-Margret K. and Laurell, Thomas},
  issn         = {1436-5073},
  keyword      = {Buffer medium exchange,radiation force,Acoustic,Erythrocytes,Ultrasonic standing wave,Particle washing},
  language     = {eng},
  number       = {3-4},
  pages        = {269--277},
  publisher    = {Springer},
  series       = {Mikrochimica Acta},
  title        = {Buffer medium exchange in continuous cell and particle streams using ultrasonic standing wave focusing},
  url          = {http://dx.doi.org/10.1007/s00604-008-0084-4},
  volume       = {164},
  year         = {2009},
}