Multiple-Timescale Dynamics of Side-Chain Carboxyl and Carbonyl Groups in Proteins by C-13 Nuclear Spin Relaxation
Paquin, Raphael ; Ferrage, Fabien ; Mulder, Frans LU ; Akke, Mikael LU
and Bodenhausen, Geoffrey
(2008)
In Journal of the American Chemical Society
130(47).
p.15805-15805
- Abstract
- Side-chain carboxyl and carbonyl groups play a major role in protein interactions and enzyme catalysis. A series of C-13 relaxation experiments is introduced to study the dynamics of carboxyl and carbonyl groups in protein side chains on both fast (sub-ns) and slower (mu s-ms) time scales. This approach is illustrated on the protein calbindin D-9k. Fast dynamics features correlate with hydrogen- and ion-binding patterns. We also identify chemical dynamics on mu s time scales in solvent-exposed carboxyl groups, most probably due to exchange between the carboxylate and carboxylic acid forms.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1375592
- author
- Paquin, Raphael
; Ferrage, Fabien
; Mulder, Frans
LU
; Akke, Mikael
LU
and Bodenhausen, Geoffrey
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of the American Chemical Society
- volume
- 130
- issue
- 47
- pages
- 15805 - 15805
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000263319600031
- scopus:56749180963
- pmid:18975903
- ISSN
- 1520-5126
- DOI
- 10.1021/ja803794g
- language
- English
- LU publication?
- yes
- id
- e015a054-3489-4343-8ea9-5aee733962f5 (old id 1375592)
- date added to LUP
- 2016-04-01 14:25:12
- date last changed
- 2022-01-28 00:29:49
@article{e015a054-3489-4343-8ea9-5aee733962f5,
abstract = {{Side-chain carboxyl and carbonyl groups play a major role in protein interactions and enzyme catalysis. A series of C-13 relaxation experiments is introduced to study the dynamics of carboxyl and carbonyl groups in protein side chains on both fast (sub-ns) and slower (mu s-ms) time scales. This approach is illustrated on the protein calbindin D-9k. Fast dynamics features correlate with hydrogen- and ion-binding patterns. We also identify chemical dynamics on mu s time scales in solvent-exposed carboxyl groups, most probably due to exchange between the carboxylate and carboxylic acid forms.}},
author = {{Paquin, Raphael and Ferrage, Fabien and Mulder, Frans and Akke, Mikael and Bodenhausen, Geoffrey}},
issn = {{1520-5126}},
language = {{eng}},
number = {{47}},
pages = {{15805--15805}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Journal of the American Chemical Society}},
title = {{Multiple-Timescale Dynamics of Side-Chain Carboxyl and Carbonyl Groups in Proteins by C-13 Nuclear Spin Relaxation}},
url = {{http://dx.doi.org/10.1021/ja803794g}},
doi = {{10.1021/ja803794g}},
volume = {{130}},
year = {{2008}},
}