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Multiple-Timescale Dynamics of Side-Chain Carboxyl and Carbonyl Groups in Proteins by C-13 Nuclear Spin Relaxation

Paquin, Raphael; Ferrage, Fabien; Mulder, Frans LU ; Akke, Mikael LU and Bodenhausen, Geoffrey (2008) In Journal of the American Chemical Society 130(47). p.15805-15805
Abstract
Side-chain carboxyl and carbonyl groups play a major role in protein interactions and enzyme catalysis. A series of C-13 relaxation experiments is introduced to study the dynamics of carboxyl and carbonyl groups in protein side chains on both fast (sub-ns) and slower (mu s-ms) time scales. This approach is illustrated on the protein calbindin D-9k. Fast dynamics features correlate with hydrogen- and ion-binding patterns. We also identify chemical dynamics on mu s time scales in solvent-exposed carboxyl groups, most probably due to exchange between the carboxylate and carboxylic acid forms.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of the American Chemical Society
volume
130
issue
47
pages
15805 - 15805
publisher
The American Chemical Society
external identifiers
  • wos:000263319600031
  • scopus:56749180963
ISSN
1520-5126
DOI
10.1021/ja803794g
language
English
LU publication?
yes
id
e015a054-3489-4343-8ea9-5aee733962f5 (old id 1375592)
date added to LUP
2009-05-08 15:33:14
date last changed
2017-10-22 04:19:42
@article{e015a054-3489-4343-8ea9-5aee733962f5,
  abstract     = {Side-chain carboxyl and carbonyl groups play a major role in protein interactions and enzyme catalysis. A series of C-13 relaxation experiments is introduced to study the dynamics of carboxyl and carbonyl groups in protein side chains on both fast (sub-ns) and slower (mu s-ms) time scales. This approach is illustrated on the protein calbindin D-9k. Fast dynamics features correlate with hydrogen- and ion-binding patterns. We also identify chemical dynamics on mu s time scales in solvent-exposed carboxyl groups, most probably due to exchange between the carboxylate and carboxylic acid forms.},
  author       = {Paquin, Raphael and Ferrage, Fabien and Mulder, Frans and Akke, Mikael and Bodenhausen, Geoffrey},
  issn         = {1520-5126},
  language     = {eng},
  number       = {47},
  pages        = {15805--15805},
  publisher    = {The American Chemical Society},
  series       = {Journal of the American Chemical Society},
  title        = {Multiple-Timescale Dynamics of Side-Chain Carboxyl and Carbonyl Groups in Proteins by C-13 Nuclear Spin Relaxation},
  url          = {http://dx.doi.org/10.1021/ja803794g},
  volume       = {130},
  year         = {2008},
}