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The use of light and electron microscopy to assess the impact of ozone on Norway spruce needles

Kivimäenpää, M; Jönsson, Anna Maria LU ; Stjernquist, Ingrid LU ; Sellden, G and Sutinen, S (2004) In Environmental Pollution 127(3). p.441-453
Abstract
Light (LM) and transmission electron (TEM) microscopy were used to study previously specified ozone symptoms in the foliage of Norway spruce. The three youngest green needle generations from twenty mature trees in two stands on sites of different soil fertility at Asa, southern Sweden, were sampled in 1999. The critical dose of ozone, expressed as AOT40, was 6362 ppb.h. LM showed ozone-specific symptoms: decreased chloroplast size with electron dense stroma advancing gradually from the outer to the inner cell layers, being most severe in the needle side facing the sky. The symptoms were expressed as ozone syndrome indices at the needle generation, tree and stand levels. The index had higher values at the low fertility site. TEM was used to... (More)
Light (LM) and transmission electron (TEM) microscopy were used to study previously specified ozone symptoms in the foliage of Norway spruce. The three youngest green needle generations from twenty mature trees in two stands on sites of different soil fertility at Asa, southern Sweden, were sampled in 1999. The critical dose of ozone, expressed as AOT40, was 6362 ppb.h. LM showed ozone-specific symptoms: decreased chloroplast size with electron dense stroma advancing gradually from the outer to the inner cell layers, being most severe in the needle side facing the sky. The symptoms were expressed as ozone syndrome indices at the needle generation, tree and stand levels. The index had higher values at the low fertility site. TEM was used to confirm the LM results. The study shows that LM can be used for diagnosis of the impact of ozone on conifers in the field. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Environmental Pollution
volume
127
issue
3
pages
441 - 453
publisher
Elsevier
external identifiers
  • wos:000187864000015
  • pmid:14638305
  • scopus:9144241850
ISSN
0269-7491
DOI
10.1016/j.envpol.2003.08.014
language
English
LU publication?
yes
id
281d3470-529f-4278-b699-1fe44fa2f103 (old id 137570)
date added to LUP
2007-07-02 11:55:28
date last changed
2017-12-10 04:35:49
@article{281d3470-529f-4278-b699-1fe44fa2f103,
  abstract     = {Light (LM) and transmission electron (TEM) microscopy were used to study previously specified ozone symptoms in the foliage of Norway spruce. The three youngest green needle generations from twenty mature trees in two stands on sites of different soil fertility at Asa, southern Sweden, were sampled in 1999. The critical dose of ozone, expressed as AOT40, was 6362 ppb.h. LM showed ozone-specific symptoms: decreased chloroplast size with electron dense stroma advancing gradually from the outer to the inner cell layers, being most severe in the needle side facing the sky. The symptoms were expressed as ozone syndrome indices at the needle generation, tree and stand levels. The index had higher values at the low fertility site. TEM was used to confirm the LM results. The study shows that LM can be used for diagnosis of the impact of ozone on conifers in the field.},
  author       = {Kivimäenpää, M and Jönsson, Anna Maria and Stjernquist, Ingrid and Sellden, G and Sutinen, S},
  issn         = {0269-7491},
  language     = {eng},
  number       = {3},
  pages        = {441--453},
  publisher    = {Elsevier},
  series       = {Environmental Pollution},
  title        = {The use of light and electron microscopy to assess the impact of ozone on Norway spruce needles},
  url          = {http://dx.doi.org/10.1016/j.envpol.2003.08.014},
  volume       = {127},
  year         = {2004},
}