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Increasing the sensitivity of the N-15 root bioassay technique: Suggested procedures

Rosengren, Ulrika LU ; Sleep, D; Jones, H E and Thelin, Gunnar LU (2003) In Communications in Soil Science and Plant Analysis 34(15-16). p.2363-2373
Abstract
The diagnosis of tree nutrient demand for nitrogen (N), phosphorus (P), and potassium (K) is possible using a root bioassay with excised roots, to measure the rate of uptake of the corresponding isotope. In this study, Norway spruce (Picea abies) roots were used to test the uptake response in roots of different diameter. The 15 N bioassay has been developed to test for nitrogen demand by plants, where high 15 N uptake demonstrates nitrogen limitation. Sometimes sample variability may mask potential treatment differences, but we have shown that when the fine root fraction of <1 mm diameter is selected rather than the whole root, the sensitivity can be significantly increased. Roots of different diameters revealed a 2-50 fold difference... (More)
The diagnosis of tree nutrient demand for nitrogen (N), phosphorus (P), and potassium (K) is possible using a root bioassay with excised roots, to measure the rate of uptake of the corresponding isotope. In this study, Norway spruce (Picea abies) roots were used to test the uptake response in roots of different diameter. The 15 N bioassay has been developed to test for nitrogen demand by plants, where high 15 N uptake demonstrates nitrogen limitation. Sometimes sample variability may mask potential treatment differences, but we have shown that when the fine root fraction of <1 mm diameter is selected rather than the whole root, the sensitivity can be significantly increased. Roots of different diameters revealed a 2-50 fold difference in the response to the N bioassay. If samples are restricted to the fine roots, the method is also more comparable to the bioassays for P and K. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Communications in Soil Science and Plant Analysis
volume
34
issue
15-16
pages
2363 - 2373
publisher
Taylor & Francis
external identifiers
  • wos:000185066600023
ISSN
0010-3624
DOI
language
English
LU publication?
yes
id
1f92b278-d3c2-4441-9777-5088ca5ff63f (old id 137623)
date added to LUP
2007-07-02 13:54:07
date last changed
2018-05-29 10:33:42
@article{1f92b278-d3c2-4441-9777-5088ca5ff63f,
  abstract     = {The diagnosis of tree nutrient demand for nitrogen (N), phosphorus (P), and potassium (K) is possible using a root bioassay with excised roots, to measure the rate of uptake of the corresponding isotope. In this study, Norway spruce (Picea abies) roots were used to test the uptake response in roots of different diameter. The 15 N bioassay has been developed to test for nitrogen demand by plants, where high 15 N uptake demonstrates nitrogen limitation. Sometimes sample variability may mask potential treatment differences, but we have shown that when the fine root fraction of &lt;1 mm diameter is selected rather than the whole root, the sensitivity can be significantly increased. Roots of different diameters revealed a 2-50 fold difference in the response to the N bioassay. If samples are restricted to the fine roots, the method is also more comparable to the bioassays for P and K.},
  author       = {Rosengren, Ulrika and Sleep, D and Jones, H E and Thelin, Gunnar},
  issn         = {0010-3624},
  language     = {eng},
  number       = {15-16},
  pages        = {2363--2373},
  publisher    = {Taylor & Francis},
  series       = {Communications in Soil Science and Plant Analysis},
  title        = {Increasing the sensitivity of the N-15 root bioassay technique: Suggested procedures},
  url          = {http://dx.doi.org/},
  volume       = {34},
  year         = {2003},
}