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Dietary Sphingomyelin Inhibits Colonic Tumorigenesis with an Up-regulation of Alkaline Sphingomyelinase Expression in ICR Mice

Zhang, Ping; Li, Baixiang; Gao, Shuying and Duan, Rui-Dong LU (2008) In Anticancer Research 28(6A). p.3631-3635
Abstract
Background: Sphingomyelin (SM) hydrolysis generates biologically active products regulating cell growth, differentiation and apoptosis. Dietary SM has been found to inhibit colonic tumorigenesis. Alkaline sphingomyelinase (alk-SMase) is the key enzyme responsible for sphingomyelin digestion in the gut. Whether or not dietary sphingomyelin affects alk-SMase expression was examined in a colon cancer animal model. Materials and Methods: Imprinting control region (ICR) mice were injected with 1,2-dimethylhydrazine (DMH) and then fed a diet with or without SM (0.5 g/kg in diet) for 22 weeks. The colonic tumorigenesis and alk-SMase activity were determined and alk-SMase expression was examined by Western blot and PCR. Results: Dietary SM... (More)
Background: Sphingomyelin (SM) hydrolysis generates biologically active products regulating cell growth, differentiation and apoptosis. Dietary SM has been found to inhibit colonic tumorigenesis. Alkaline sphingomyelinase (alk-SMase) is the key enzyme responsible for sphingomyelin digestion in the gut. Whether or not dietary sphingomyelin affects alk-SMase expression was examined in a colon cancer animal model. Materials and Methods: Imprinting control region (ICR) mice were injected with 1,2-dimethylhydrazine (DMH) and then fed a diet with or without SM (0.5 g/kg in diet) for 22 weeks. The colonic tumorigenesis and alk-SMase activity were determined and alk-SMase expression was examined by Western blot and PCR. Results: Dietary SM inhibited the tumorigenesis and increased the alk-SMase activity in the colon by 65%. The increased activity was associated with increased enzyme protein and mRNA expression. No changes of acid and neutral sphingomyelinase activities were found. Conclusion: Long-term supplementation with dietary sphingomyelin up-regulates colonic alk-SMase expression, which may contribute to the inhibitory effects of sphingomyelin against colonic carcinogenesis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Sphingomyelin, alkaline sphingomyelinase, colon cancer, mouse
in
Anticancer Research
volume
28
issue
6A
pages
3631 - 3635
publisher
International Institute of Cancer Research
external identifiers
  • wos:000262049100010
  • scopus:58149165342
ISSN
1791-7530
language
English
LU publication?
yes
id
72c2c329-1ee8-4983-961d-4a4283fabb58 (old id 1376273)
date added to LUP
2009-04-24 12:29:01
date last changed
2017-11-12 03:20:51
@article{72c2c329-1ee8-4983-961d-4a4283fabb58,
  abstract     = {Background: Sphingomyelin (SM) hydrolysis generates biologically active products regulating cell growth, differentiation and apoptosis. Dietary SM has been found to inhibit colonic tumorigenesis. Alkaline sphingomyelinase (alk-SMase) is the key enzyme responsible for sphingomyelin digestion in the gut. Whether or not dietary sphingomyelin affects alk-SMase expression was examined in a colon cancer animal model. Materials and Methods: Imprinting control region (ICR) mice were injected with 1,2-dimethylhydrazine (DMH) and then fed a diet with or without SM (0.5 g/kg in diet) for 22 weeks. The colonic tumorigenesis and alk-SMase activity were determined and alk-SMase expression was examined by Western blot and PCR. Results: Dietary SM inhibited the tumorigenesis and increased the alk-SMase activity in the colon by 65%. The increased activity was associated with increased enzyme protein and mRNA expression. No changes of acid and neutral sphingomyelinase activities were found. Conclusion: Long-term supplementation with dietary sphingomyelin up-regulates colonic alk-SMase expression, which may contribute to the inhibitory effects of sphingomyelin against colonic carcinogenesis.},
  author       = {Zhang, Ping and Li, Baixiang and Gao, Shuying and Duan, Rui-Dong},
  issn         = {1791-7530},
  keyword      = {Sphingomyelin,alkaline sphingomyelinase,colon cancer,mouse},
  language     = {eng},
  number       = {6A},
  pages        = {3631--3635},
  publisher    = {International Institute of Cancer Research},
  series       = {Anticancer Research},
  title        = {Dietary Sphingomyelin Inhibits Colonic Tumorigenesis with an Up-regulation of Alkaline Sphingomyelinase Expression in ICR Mice},
  volume       = {28},
  year         = {2008},
}