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First attempt to produce experimental Campylobacter concisus infection in mice

Aabenhus, Rune; Stenram, Unne LU ; Andersen, Leif Percival; Permin, Henrik and Ljungh, Åsa LU (2008) In World Journal of Gastroenterology 14(45). p.6954-6959
Abstract
AIM: To infect mice with atypical Campylobacter concisus (C concisus) for the first time. METHODS: Three separate experiments were conducted in order to screen the ability of five clinical C concisus isolates of intestinal origin and the ATCC 33237 type strain of oral origin to colonize and produce infection in immunocompetent BALB/cA mice. The majority of the BALB/cA mice were treated with cyclophosphamide prior to C concisus inoculation to suppress immune functions. Inoculation of C concisus was performed by the gastric route. RESULTS: C concisus was isolated from the liver, ileum and jejunum of cyclophosphamide-treated mice in the first experiment. No C concisus strains were isolated in the two subsequent experiments. Mice infected with... (More)
AIM: To infect mice with atypical Campylobacter concisus (C concisus) for the first time. METHODS: Three separate experiments were conducted in order to screen the ability of five clinical C concisus isolates of intestinal origin and the ATCC 33237 type strain of oral origin to colonize and produce infection in immunocompetent BALB/cA mice. The majority of the BALB/cA mice were treated with cyclophosphamide prior to C concisus inoculation to suppress immune functions. Inoculation of C concisus was performed by the gastric route. RESULTS: C concisus was isolated from the liver, ileum and jejunum of cyclophosphamide-treated mice in the first experiment. No C concisus strains were isolated in the two subsequent experiments. Mice infected with C concisus showed a significant loss of body weight from day two through to day five of infection but this decreased at the end of the first week. Histopathologicalexamination did not consistently find signs of inflammation in the gut, but occasionally microabscesses were found in the liver of infected animals. CONCLUSION: Transient colonization with C concisus was observed in mice with loss of body weight. Future studies should concentrate on the first few days after inoculation and in other strains of mice. (C) 2008 The WJG Press. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Campylobacter concisus, Animal model, BALB/cA mice, Infection, Colonization
in
World Journal of Gastroenterology
volume
14
issue
45
pages
6954 - 6959
publisher
WJG Press
external identifiers
  • wos:000261693300010
  • scopus:63449099320
ISSN
1007-9327
DOI
10.3748/wjg.14.6954
language
English
LU publication?
yes
id
a9b5342a-105e-4908-b468-2c1755d50cb1 (old id 1377504)
date added to LUP
2009-04-24 09:48:44
date last changed
2017-09-17 05:00:24
@article{a9b5342a-105e-4908-b468-2c1755d50cb1,
  abstract     = {AIM: To infect mice with atypical Campylobacter concisus (C concisus) for the first time. METHODS: Three separate experiments were conducted in order to screen the ability of five clinical C concisus isolates of intestinal origin and the ATCC 33237 type strain of oral origin to colonize and produce infection in immunocompetent BALB/cA mice. The majority of the BALB/cA mice were treated with cyclophosphamide prior to C concisus inoculation to suppress immune functions. Inoculation of C concisus was performed by the gastric route. RESULTS: C concisus was isolated from the liver, ileum and jejunum of cyclophosphamide-treated mice in the first experiment. No C concisus strains were isolated in the two subsequent experiments. Mice infected with C concisus showed a significant loss of body weight from day two through to day five of infection but this decreased at the end of the first week. Histopathologicalexamination did not consistently find signs of inflammation in the gut, but occasionally microabscesses were found in the liver of infected animals. CONCLUSION: Transient colonization with C concisus was observed in mice with loss of body weight. Future studies should concentrate on the first few days after inoculation and in other strains of mice. (C) 2008 The WJG Press. All rights reserved.},
  author       = {Aabenhus, Rune and Stenram, Unne and Andersen, Leif Percival and Permin, Henrik and Ljungh, Åsa},
  issn         = {1007-9327},
  keyword      = {Campylobacter concisus,Animal model,BALB/cA mice,Infection,Colonization},
  language     = {eng},
  number       = {45},
  pages        = {6954--6959},
  publisher    = {WJG Press},
  series       = {World Journal of Gastroenterology},
  title        = {First attempt to produce experimental Campylobacter concisus infection in mice},
  url          = {http://dx.doi.org/10.3748/wjg.14.6954},
  volume       = {14},
  year         = {2008},
}