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Methods to Detect Loss of Lysosomal Membrane Integrity

Aits, Sonja LU (2019) In Methods in Molecular Biology 1880. p.315-329
Abstract
Loss of lysosomal membrane integrity, often referred to as lysosomal membrane permeabilization (LMP), occurs in many instances of cell death either as an initiating or as an amplifying event. Currently, the best method for detecting LMP is the galectin puncta formation assay which can be used for a broad range of sample types, both fixed and live, is easy to perform, and highly sensitive. This method, which is similar to the widely used LC3 puncta formation assay for autophagy, is based on the translocation of galectins to damaged lysosomes resulting in a change from uniform to punctate staining pattern. Here, we provide protocols for the galectin puncta formation assay in fixed and live cells and for an alternative assay based on... (More)
Loss of lysosomal membrane integrity, often referred to as lysosomal membrane permeabilization (LMP), occurs in many instances of cell death either as an initiating or as an amplifying event. Currently, the best method for detecting LMP is the galectin puncta formation assay which can be used for a broad range of sample types, both fixed and live, is easy to perform, and highly sensitive. This method, which is similar to the widely used LC3 puncta formation assay for autophagy, is based on the translocation of galectins to damaged lysosomes resulting in a change from uniform to punctate staining pattern. Here, we provide protocols for the galectin puncta formation assay in fixed and live cells and for an alternative assay based on fluorescent dextran release from damaged lysosomes, which can be performed in parallel (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Autophagy
series title
Methods in Molecular Biology
editor
Ktistakis, Nicholas; Florey, Oliver; and
volume
1880
pages
315 - 329
publisher
Humana Press
ISSN
1064-3745
ISBN
978149398873-0
DOI
10.1007/978-1-4939-8873-0
language
English
LU publication?
yes
id
137a5acd-52dd-4ac5-b0be-a2c94767f2a5
date added to LUP
2019-01-09 12:42:26
date last changed
2019-01-09 15:09:56
@inbook{137a5acd-52dd-4ac5-b0be-a2c94767f2a5,
  abstract     = {Loss of lysosomal membrane integrity, often referred to as lysosomal membrane permeabilization (LMP), occurs in many instances of cell death either as an initiating or as an amplifying event. Currently, the best method for detecting LMP is the galectin puncta formation assay which can be used for a broad range of sample types, both fixed and live, is easy to perform, and highly sensitive. This method, which is similar to the widely used LC3 puncta formation assay for autophagy, is based on the translocation of galectins to damaged lysosomes resulting in a change from uniform to punctate staining pattern. Here, we provide protocols for the galectin puncta formation assay in fixed and live cells and for an alternative assay based on fluorescent dextran release from damaged lysosomes, which can be performed in parallel},
  author       = {Aits, Sonja},
  editor       = {Ktistakis, Nicholas and Florey, Oliver},
  isbn         = {978149398873-0},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {315--329},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Methods to Detect Loss of Lysosomal Membrane Integrity},
  url          = {http://dx.doi.org/10.1007/978-1-4939-8873-0},
  volume       = {1880},
  year         = {2019},
}