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Fusion of the EWSR1 and ATF1 genes without expression of the MITF-M transcript in angiomatoid fibrous histiocytoma

Hallor, Karolin Hansén LU ; Mertens, Fredrik LU ; Jin, Yuesheng LU ; Meis-Kindblom, Jeanne M; Kindblom, Lars-Gunnar; Behrendtz, Mikael; Kalén, Anders; Mandahl, Nils LU and Panagopoulos, Ioannis LU (2005) In Genes, Chromosomes and Cancer 44(1). p.97-102
Abstract

Angiomatoid fibrous histiocytoma (AFH) is a rare soft tissue tumor that usually occurs in children and young adults. Only two cases of AFH with genetic rearrangements have been reported previously; both of these had a FUS-ATF1 fusion gene. We have studied an AFH from a 9-year-old boy whose tumor displayed a t(12;22)(q13;q12) as the sole cytogenetic aberration. FISH,RT-PCR, and sequence analyses revealed an EWSR1-ATF1 fusion gene that has previously been reported in clear cell sarcoma (CCS), a soft tissue sarcoma that is morphologically and clinically distinct from AFH. This study thus has demonstrated that the EWSR1-ATF1 chimera represents a fusion gene that can be associated with different tumor types. Simultaneous expression of the... (More)

Angiomatoid fibrous histiocytoma (AFH) is a rare soft tissue tumor that usually occurs in children and young adults. Only two cases of AFH with genetic rearrangements have been reported previously; both of these had a FUS-ATF1 fusion gene. We have studied an AFH from a 9-year-old boy whose tumor displayed a t(12;22)(q13;q12) as the sole cytogenetic aberration. FISH,RT-PCR, and sequence analyses revealed an EWSR1-ATF1 fusion gene that has previously been reported in clear cell sarcoma (CCS), a soft tissue sarcoma that is morphologically and clinically distinct from AFH. This study thus has demonstrated that the EWSR1-ATF1 chimera represents a fusion gene that can be associated with different tumor types. Simultaneous expression of the EWSR1-ATF1 and MITF-M transcripts in CCS has led to the proposal that the MITF-M promoter is transactivated by EWSR1-ATF1. The AFH, however, did not express the MITF-M transcript, supporting the theory that MITF-M expression in CCS is a reflection of its cellular origin, rather than a consequence of the presence of an EWSR1-ATF1 fusion protein. Activation of the EWSR1-ATF1 oncogene is probably an early step in the transformation process, but the overall gene expression patterns are likely to vary considerably between AFH and CCS, in keeping with their clinicopathologic differences.

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keywords
Activating Transcription Factor 1, Adenocarcinoma, Clear Cell, Artificial Gene Fusion, Base Sequence, Calmodulin-Binding Proteins, Child, Chromosomes, Human, Pair 12, Chromosomes, Human, Pair 22, DNA Primers, DNA-Binding Proteins, Histiocytoma, Benign Fibrous, Humans, Karyotyping, Male, Microphthalmia-Associated Transcription Factor, Nuclear Proteins, RNA-Binding Proteins, Sequence Deletion, Transcription Factors, Transcription, Genetic, Translocation, Genetic, Case Reports, Journal Article, Research Support, Non-U.S. Gov't
in
Genes, Chromosomes and Cancer
volume
44
issue
1
pages
6 pages
publisher
John Wiley & Sons
external identifiers
  • wos:000230781000010
  • pmid:15884099
  • scopus:23044459407
ISSN
1045-2257
DOI
10.1002/gcc.20201
language
English
LU publication?
yes
id
c05f8f4d-e574-474c-a5c4-78f9fbdd3e2d (old id 138056)
date added to LUP
2007-07-06 14:59:28
date last changed
2017-09-24 03:36:03
@article{c05f8f4d-e574-474c-a5c4-78f9fbdd3e2d,
  abstract     = {<p>Angiomatoid fibrous histiocytoma (AFH) is a rare soft tissue tumor that usually occurs in children and young adults. Only two cases of AFH with genetic rearrangements have been reported previously; both of these had a FUS-ATF1 fusion gene. We have studied an AFH from a 9-year-old boy whose tumor displayed a t(12;22)(q13;q12) as the sole cytogenetic aberration. FISH,RT-PCR, and sequence analyses revealed an EWSR1-ATF1 fusion gene that has previously been reported in clear cell sarcoma (CCS), a soft tissue sarcoma that is morphologically and clinically distinct from AFH. This study thus has demonstrated that the EWSR1-ATF1 chimera represents a fusion gene that can be associated with different tumor types. Simultaneous expression of the EWSR1-ATF1 and MITF-M transcripts in CCS has led to the proposal that the MITF-M promoter is transactivated by EWSR1-ATF1. The AFH, however, did not express the MITF-M transcript, supporting the theory that MITF-M expression in CCS is a reflection of its cellular origin, rather than a consequence of the presence of an EWSR1-ATF1 fusion protein. Activation of the EWSR1-ATF1 oncogene is probably an early step in the transformation process, but the overall gene expression patterns are likely to vary considerably between AFH and CCS, in keeping with their clinicopathologic differences.</p>},
  author       = {Hallor, Karolin Hansén and Mertens, Fredrik and Jin, Yuesheng and Meis-Kindblom, Jeanne M and Kindblom, Lars-Gunnar and Behrendtz, Mikael and Kalén, Anders and Mandahl, Nils and Panagopoulos, Ioannis},
  issn         = {1045-2257},
  keyword      = {Activating Transcription Factor 1,Adenocarcinoma, Clear Cell,Artificial Gene Fusion,Base Sequence,Calmodulin-Binding Proteins,Child,Chromosomes, Human, Pair 12,Chromosomes, Human, Pair 22,DNA Primers,DNA-Binding Proteins,Histiocytoma, Benign Fibrous,Humans,Karyotyping,Male,Microphthalmia-Associated Transcription Factor,Nuclear Proteins,RNA-Binding Proteins,Sequence Deletion,Transcription Factors,Transcription, Genetic,Translocation, Genetic,Case Reports,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {1},
  pages        = {97--102},
  publisher    = {John Wiley & Sons},
  series       = {Genes, Chromosomes and Cancer},
  title        = {Fusion of the EWSR1 and ATF1 genes without expression of the MITF-M transcript in angiomatoid fibrous histiocytoma},
  url          = {http://dx.doi.org/10.1002/gcc.20201},
  volume       = {44},
  year         = {2005},
}