Integrated protein microchip assay with dual fluorescent- and MALDI read-out
(2004) In Journal of Proteome Research 3(5). p.988-994- Abstract
- A pore chip protein array (PCPA) concept based on a dual readout configuration, fluorescence imaging, and MALDI-TOF MS has been developed. Highly packed, (>4000 Spots/cm(2)), antibody arrays were dispensed on the porous chip by using a piezo-electric microdispenser. Sandwich assay was made after blocking by addition of a secondary antibody either lgG-FITC-labeled or anti-Ang II. The antigen in the first system was a large protein (lgG), and in the other system, a FITC marked peptide Angiotensin II (Ang II) was used. Ang II antibodies showed specificity for Ang II, while the Ang I antibodies showed binding properties for Ang I, II, and Renin. Fluorescence and MALDI TOF MS read-out was made for lgG and Ang II. A major advantage of the... (More)
- A pore chip protein array (PCPA) concept based on a dual readout configuration, fluorescence imaging, and MALDI-TOF MS has been developed. Highly packed, (>4000 Spots/cm(2)), antibody arrays were dispensed on the porous chip by using a piezo-electric microdispenser. Sandwich assay was made after blocking by addition of a secondary antibody either lgG-FITC-labeled or anti-Ang II. The antigen in the first system was a large protein (lgG), and in the other system, a FITC marked peptide Angiotensin II (Ang II) was used. Ang II antibodies showed specificity for Ang II, while the Ang I antibodies showed binding properties for Ang I, II, and Renin. Fluorescence and MALDI TOF MS read-out was made for lgG and Ang II. A major advantage of the dual read-out PCPA approach is that both affinity binding and mass identity are derived. Detection limits for Ang II on the chip is as low as 500 zmol (Ang II). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/138344
- author
- Finnskog, David LU ; Ressine, Anton LU ; Laurell, Thomas LU and Marko-Varga, György LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- matrix-assisted laser desorption ionization time-of-flight
- in
- Journal of Proteome Research
- volume
- 3
- issue
- 5
- pages
- 988 - 994
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000224693800009
- scopus:7044237454
- ISSN
- 1535-3893
- DOI
- 10.1021/pr0499287
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biomedical Engineering (011200011), Analytical Chemistry (S/LTH) (011001004)
- id
- 760e48d8-3724-4cf9-8aa5-9995b4965db5 (old id 138344)
- date added to LUP
- 2016-04-01 11:41:23
- date last changed
- 2022-02-25 19:56:54
@article{760e48d8-3724-4cf9-8aa5-9995b4965db5, abstract = {{A pore chip protein array (PCPA) concept based on a dual readout configuration, fluorescence imaging, and MALDI-TOF MS has been developed. Highly packed, (>4000 Spots/cm(2)), antibody arrays were dispensed on the porous chip by using a piezo-electric microdispenser. Sandwich assay was made after blocking by addition of a secondary antibody either lgG-FITC-labeled or anti-Ang II. The antigen in the first system was a large protein (lgG), and in the other system, a FITC marked peptide Angiotensin II (Ang II) was used. Ang II antibodies showed specificity for Ang II, while the Ang I antibodies showed binding properties for Ang I, II, and Renin. Fluorescence and MALDI TOF MS read-out was made for lgG and Ang II. A major advantage of the dual read-out PCPA approach is that both affinity binding and mass identity are derived. Detection limits for Ang II on the chip is as low as 500 zmol (Ang II).}}, author = {{Finnskog, David and Ressine, Anton and Laurell, Thomas and Marko-Varga, György}}, issn = {{1535-3893}}, keywords = {{matrix-assisted laser desorption ionization time-of-flight}}, language = {{eng}}, number = {{5}}, pages = {{988--994}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Journal of Proteome Research}}, title = {{Integrated protein microchip assay with dual fluorescent- and MALDI read-out}}, url = {{http://dx.doi.org/10.1021/pr0499287}}, doi = {{10.1021/pr0499287}}, volume = {{3}}, year = {{2004}}, }