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Validation of a Diagnostic PCR Method for Routine Analysis of Salmonella spp. in Animal Feed Samples

Löfström, Charlotta LU ; Engdahl Axelsson, Charlotta and Rådström, Peter LU (2008) In Food Analytical Methods 1(1). p.23-27
Abstract
As a part of a validation study, a comparative study of a PCR method and the standard culture-based method NMKL-71, for detection of Salmonella, was performed according to the validation protocol from the Nordic validation organ for validation of alternative microbiological methods (NordVal) on 250 artificially or naturally contaminated animal feed samples. The PCR method is based on culture enrichment in buffered peptone water followed by PCR using the DNA polymerase Tth and an internal amplification control. No significant difference was found between the two methods. The relative accuracy, relative sensitivity and relative specificity were found to be 96.0, 97.3, and 98.8%, respectively. PCR inhibition was observed for rape seed... (More)
As a part of a validation study, a comparative study of a PCR method and the standard culture-based method NMKL-71, for detection of Salmonella, was performed according to the validation protocol from the Nordic validation organ for validation of alternative microbiological methods (NordVal) on 250 artificially or naturally contaminated animal feed samples. The PCR method is based on culture enrichment in buffered peptone water followed by PCR using the DNA polymerase Tth and an internal amplification control. No significant difference was found between the two methods. The relative accuracy, relative sensitivity and relative specificity were found to be 96.0, 97.3, and 98.8%, respectively. PCR inhibition was observed for rape seed samples. For the acidified feed samples, more Salmonella-positive samples were found with the PCR method compared to the NMKL method. This study focuses on the growing demand for validated diagnostic PCR methods for routine analysis of animal feed and food samples to assure safety in the food production chain. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Reaction, Polymerase Chain, PCR, Validation, Salmonella, Animal Feed, NordVal
in
Food Analytical Methods
volume
1
issue
1
pages
23 - 27
publisher
Springer
external identifiers
  • wos:000261694500005
ISSN
1936-9751
DOI
10.1007/s12161-007-9003-2
language
English
LU publication?
yes
id
78a59948-8bd5-4a16-acd7-de1dff49961b (old id 1383891)
date added to LUP
2009-04-22 11:20:45
date last changed
2016-04-15 20:34:46
@article{78a59948-8bd5-4a16-acd7-de1dff49961b,
  abstract     = {As a part of a validation study, a comparative study of a PCR method and the standard culture-based method NMKL-71, for detection of Salmonella, was performed according to the validation protocol from the Nordic validation organ for validation of alternative microbiological methods (NordVal) on 250 artificially or naturally contaminated animal feed samples. The PCR method is based on culture enrichment in buffered peptone water followed by PCR using the DNA polymerase Tth and an internal amplification control. No significant difference was found between the two methods. The relative accuracy, relative sensitivity and relative specificity were found to be 96.0, 97.3, and 98.8%, respectively. PCR inhibition was observed for rape seed samples. For the acidified feed samples, more Salmonella-positive samples were found with the PCR method compared to the NMKL method. This study focuses on the growing demand for validated diagnostic PCR methods for routine analysis of animal feed and food samples to assure safety in the food production chain.},
  author       = {Löfström, Charlotta and Engdahl Axelsson, Charlotta and Rådström, Peter},
  issn         = {1936-9751},
  keyword      = {Reaction,Polymerase Chain,PCR,Validation,Salmonella,Animal Feed,NordVal},
  language     = {eng},
  number       = {1},
  pages        = {23--27},
  publisher    = {Springer},
  series       = {Food Analytical Methods},
  title        = {Validation of a Diagnostic PCR Method for Routine Analysis of Salmonella spp. in Animal Feed Samples},
  url          = {http://dx.doi.org/10.1007/s12161-007-9003-2},
  volume       = {1},
  year         = {2008},
}