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Dietary Supplementation With Phytohemagglutinin In Combination With Alpha-Ketoglutarate Limits The Excretion Of Nitrogen Via Urinary Tract

Filip, Rafal; Wdowiak, Leszek; Harrison, Adrian P. and Pierzynowski, Stefan LU (2008) In Annals of Agricultural and Environmental Medicine 15(2). p.309-315
Abstract
The aim of the study was to evaluate the effect of both phytohaemagglutinin (PHA) alone, and in combination with alpha-ketoglutaric acid (AKG), on nitrogen elimination via the urinary tract as opposed to the gastrointestinal tract of rats. In experiment I, rats were assigned to one of two experimental groups, (1) Control and (2) PHA, whilst in experiment 2, rats were assigned to one of three experimental groups, (1) Control, (2) AKG, and (3) AKG+PHA. AKG was administered via drinking water, while PHA was administered via a stomach tube. The stock solution of crude PHA in 0.9% NaCl, was (20% w/v) in water: 50 mg PHA/ml, 20 ml/kg body wt. Rats were 7 weeks old at the start of the experiments. Significantly lower daily weight gains in the... (More)
The aim of the study was to evaluate the effect of both phytohaemagglutinin (PHA) alone, and in combination with alpha-ketoglutaric acid (AKG), on nitrogen elimination via the urinary tract as opposed to the gastrointestinal tract of rats. In experiment I, rats were assigned to one of two experimental groups, (1) Control and (2) PHA, whilst in experiment 2, rats were assigned to one of three experimental groups, (1) Control, (2) AKG, and (3) AKG+PHA. AKG was administered via drinking water, while PHA was administered via a stomach tube. The stock solution of crude PHA in 0.9% NaCl, was (20% w/v) in water: 50 mg PHA/ml, 20 ml/kg body wt. Rats were 7 weeks old at the start of the experiments. Significantly lower daily weight gains in the AKG+PHA and PHA groups (p<0.05) were observed compared to the Control and AKG groups. Increased duodenal crypt depth (138%; p<0.05) was noticeable in the AKG+PHA group of Controls; however, there was no significant difference in the thickness of the tunica mucosa. In the AKG+PHA group, the expression of neuropeptide Y (NPY) in the granula of neuronal cells of the submucosal parasympathetic ganglia was noticeable, although no expression was found in goblet cells. Finally, significant reduction in N excretion in urine was observed in the AKG+PHA, compared with the Control groups (p<0.05). It is concluded that a combined PHA and AKG treatment stimulated the small bowel growth via enhanced epithelial turnover, reduced the N excreted in urine and increased the N in faeces. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
nitrogen elimination, in-, alpha-ketoglutaric acid, phytohemagglutinin
in
Annals of Agricultural and Environmental Medicine
volume
15
issue
2
pages
309 - 315
publisher
Institute of Agricultural Medicine in Lublin, Poland
external identifiers
  • wos:000261767500021
ISSN
1898-2263
language
English
LU publication?
yes
id
515c948e-8af1-4e0c-a70e-877dfea2acfd (old id 1384063)
date added to LUP
2009-05-08 13:28:51
date last changed
2016-04-15 20:42:27
@article{515c948e-8af1-4e0c-a70e-877dfea2acfd,
  abstract     = {The aim of the study was to evaluate the effect of both phytohaemagglutinin (PHA) alone, and in combination with alpha-ketoglutaric acid (AKG), on nitrogen elimination via the urinary tract as opposed to the gastrointestinal tract of rats. In experiment I, rats were assigned to one of two experimental groups, (1) Control and (2) PHA, whilst in experiment 2, rats were assigned to one of three experimental groups, (1) Control, (2) AKG, and (3) AKG+PHA. AKG was administered via drinking water, while PHA was administered via a stomach tube. The stock solution of crude PHA in 0.9% NaCl, was (20% w/v) in water: 50 mg PHA/ml, 20 ml/kg body wt. Rats were 7 weeks old at the start of the experiments. Significantly lower daily weight gains in the AKG+PHA and PHA groups (p&lt;0.05) were observed compared to the Control and AKG groups. Increased duodenal crypt depth (138%; p&lt;0.05) was noticeable in the AKG+PHA group of Controls; however, there was no significant difference in the thickness of the tunica mucosa. In the AKG+PHA group, the expression of neuropeptide Y (NPY) in the granula of neuronal cells of the submucosal parasympathetic ganglia was noticeable, although no expression was found in goblet cells. Finally, significant reduction in N excretion in urine was observed in the AKG+PHA, compared with the Control groups (p&lt;0.05). It is concluded that a combined PHA and AKG treatment stimulated the small bowel growth via enhanced epithelial turnover, reduced the N excreted in urine and increased the N in faeces.},
  author       = {Filip, Rafal and Wdowiak, Leszek and Harrison, Adrian P. and Pierzynowski, Stefan},
  issn         = {1898-2263},
  keyword      = {nitrogen elimination,in-,alpha-ketoglutaric acid,phytohemagglutinin},
  language     = {eng},
  number       = {2},
  pages        = {309--315},
  publisher    = {Institute of Agricultural Medicine in Lublin, Poland},
  series       = {Annals of Agricultural and Environmental Medicine},
  title        = {Dietary Supplementation With Phytohemagglutinin In Combination With Alpha-Ketoglutarate Limits The Excretion Of Nitrogen Via Urinary Tract},
  volume       = {15},
  year         = {2008},
}