In situ poly(ethylene imine) coating of hollow fiber membranes used for microdialysis sampling
(2004) In Pure and Applied Chemistry 76(4). p.879-888- Abstract
- A method for the in situ modification of hollow fiber membranes used as sampling units for microdialysis probes is presented. The method consists of adsorption-coating, high-molecular-weight poly(ethylene imine), PEI, onto membranes, already fitted on microdialysis probes. Modification of membranes was designed to specifically explore the so-called Andrade effects and thus enhance the interaction of membranes with enzyme. The performances of polysulfone, polyethersulfone, and polyamide membranes modified with PEI-enzyrne complex were evaluated based on the membrane extraction fraction for maltose and maltotriose and membrane morphology as examined by scanning electron microscopy. Of the membranes tested, the PEI-enzyme complex least... (More)
- A method for the in situ modification of hollow fiber membranes used as sampling units for microdialysis probes is presented. The method consists of adsorption-coating, high-molecular-weight poly(ethylene imine), PEI, onto membranes, already fitted on microdialysis probes. Modification of membranes was designed to specifically explore the so-called Andrade effects and thus enhance the interaction of membranes with enzyme. The performances of polysulfone, polyethersulfone, and polyamide membranes modified with PEI-enzyrne complex were evaluated based on the membrane extraction fraction for maltose and maltotriose and membrane morphology as examined by scanning electron microscopy. Of the membranes tested, the PEI-enzyme complex least affected the performance of the polysulfone membranes. Conversion of maltoheptaose to maltotriose and maltose was increased reproducibly (within a 5 % relative standard deviation) by 50 % for modified membranes compared to the native hollow fiber membranes. The results demonstrate the potential to effectively modify membranes already fitted on a microdialysis probe. Such a procedure can be modified and employed to either promote or reduce membrane-protein interaction for hollow fibers used as microdialysis sampling units or other similar membrane applications. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/138508
- author
- Torto, N ; Ohlrogge, M ; Gorton, Lo LU ; Van Alstine, J M ; Laurell, Thomas LU and Marko-Varga, György LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Pure and Applied Chemistry
- volume
- 76
- issue
- 4
- pages
- 879 - 888
- publisher
- IUPAC
- external identifiers
-
- wos:000222017100015
- scopus:2942674846
- ISSN
- 0033-4545
- DOI
- 10.1351/pac200476040879
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Biomedical Engineering (011200011)
- id
- a0ff9991-0a3b-40e6-aa6c-121f8828ac57 (old id 138508)
- date added to LUP
- 2016-04-01 16:22:56
- date last changed
- 2022-01-28 19:17:47
@article{a0ff9991-0a3b-40e6-aa6c-121f8828ac57, abstract = {{A method for the in situ modification of hollow fiber membranes used as sampling units for microdialysis probes is presented. The method consists of adsorption-coating, high-molecular-weight poly(ethylene imine), PEI, onto membranes, already fitted on microdialysis probes. Modification of membranes was designed to specifically explore the so-called Andrade effects and thus enhance the interaction of membranes with enzyme. The performances of polysulfone, polyethersulfone, and polyamide membranes modified with PEI-enzyrne complex were evaluated based on the membrane extraction fraction for maltose and maltotriose and membrane morphology as examined by scanning electron microscopy. Of the membranes tested, the PEI-enzyme complex least affected the performance of the polysulfone membranes. Conversion of maltoheptaose to maltotriose and maltose was increased reproducibly (within a 5 % relative standard deviation) by 50 % for modified membranes compared to the native hollow fiber membranes. The results demonstrate the potential to effectively modify membranes already fitted on a microdialysis probe. Such a procedure can be modified and employed to either promote or reduce membrane-protein interaction for hollow fibers used as microdialysis sampling units or other similar membrane applications.}}, author = {{Torto, N and Ohlrogge, M and Gorton, Lo and Van Alstine, J M and Laurell, Thomas and Marko-Varga, György}}, issn = {{0033-4545}}, language = {{eng}}, number = {{4}}, pages = {{879--888}}, publisher = {{IUPAC}}, series = {{Pure and Applied Chemistry}}, title = {{In situ poly(ethylene imine) coating of hollow fiber membranes used for microdialysis sampling}}, url = {{http://dx.doi.org/10.1351/pac200476040879}}, doi = {{10.1351/pac200476040879}}, volume = {{76}}, year = {{2004}}, }