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In situ poly(ethylene imine) coating of hollow fiber membranes used for microdialysis sampling

Torto, N; Ohlrogge, M; Gorton, Lo LU ; Van Alstine, J M; Laurell, Thomas LU and Marko-Varga, György LU (2004) In Pure and Applied Chemistry 76(4). p.879-888
Abstract
A method for the in situ modification of hollow fiber membranes used as sampling units for microdialysis probes is presented. The method consists of adsorption-coating, high-molecular-weight poly(ethylene imine), PEI, onto membranes, already fitted on microdialysis probes. Modification of membranes was designed to specifically explore the so-called Andrade effects and thus enhance the interaction of membranes with enzyme. The performances of polysulfone, polyethersulfone, and polyamide membranes modified with PEI-enzyrne complex were evaluated based on the membrane extraction fraction for maltose and maltotriose and membrane morphology as examined by scanning electron microscopy. Of the membranes tested, the PEI-enzyme complex least... (More)
A method for the in situ modification of hollow fiber membranes used as sampling units for microdialysis probes is presented. The method consists of adsorption-coating, high-molecular-weight poly(ethylene imine), PEI, onto membranes, already fitted on microdialysis probes. Modification of membranes was designed to specifically explore the so-called Andrade effects and thus enhance the interaction of membranes with enzyme. The performances of polysulfone, polyethersulfone, and polyamide membranes modified with PEI-enzyrne complex were evaluated based on the membrane extraction fraction for maltose and maltotriose and membrane morphology as examined by scanning electron microscopy. Of the membranes tested, the PEI-enzyme complex least affected the performance of the polysulfone membranes. Conversion of maltoheptaose to maltotriose and maltose was increased reproducibly (within a 5 % relative standard deviation) by 50 % for modified membranes compared to the native hollow fiber membranes. The results demonstrate the potential to effectively modify membranes already fitted on a microdialysis probe. Such a procedure can be modified and employed to either promote or reduce membrane-protein interaction for hollow fibers used as microdialysis sampling units or other similar membrane applications. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Pure and Applied Chemistry
volume
76
issue
4
pages
879 - 888
publisher
IUPAC
external identifiers
  • wos:000222017100015
  • scopus:2942674846
ISSN
0033-4545
DOI
10.1351/pac200476040879
language
English
LU publication?
yes
id
a0ff9991-0a3b-40e6-aa6c-121f8828ac57 (old id 138508)
date added to LUP
2007-06-28 09:12:31
date last changed
2017-01-01 07:03:45
@article{a0ff9991-0a3b-40e6-aa6c-121f8828ac57,
  abstract     = {A method for the in situ modification of hollow fiber membranes used as sampling units for microdialysis probes is presented. The method consists of adsorption-coating, high-molecular-weight poly(ethylene imine), PEI, onto membranes, already fitted on microdialysis probes. Modification of membranes was designed to specifically explore the so-called Andrade effects and thus enhance the interaction of membranes with enzyme. The performances of polysulfone, polyethersulfone, and polyamide membranes modified with PEI-enzyrne complex were evaluated based on the membrane extraction fraction for maltose and maltotriose and membrane morphology as examined by scanning electron microscopy. Of the membranes tested, the PEI-enzyme complex least affected the performance of the polysulfone membranes. Conversion of maltoheptaose to maltotriose and maltose was increased reproducibly (within a 5 % relative standard deviation) by 50 % for modified membranes compared to the native hollow fiber membranes. The results demonstrate the potential to effectively modify membranes already fitted on a microdialysis probe. Such a procedure can be modified and employed to either promote or reduce membrane-protein interaction for hollow fibers used as microdialysis sampling units or other similar membrane applications.},
  author       = {Torto, N and Ohlrogge, M and Gorton, Lo and Van Alstine, J M and Laurell, Thomas and Marko-Varga, György},
  issn         = {0033-4545},
  language     = {eng},
  number       = {4},
  pages        = {879--888},
  publisher    = {IUPAC},
  series       = {Pure and Applied Chemistry},
  title        = {In situ poly(ethylene imine) coating of hollow fiber membranes used for microdialysis sampling},
  url          = {http://dx.doi.org/10.1351/pac200476040879},
  volume       = {76},
  year         = {2004},
}