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Recombinant horseradish peroxidase- and cytochrome c-based two-electrode system for detection of superoxide radicals

Shipovskov, Stepan LU ; Ferapontova, Elena LU ; Gazaryan, I and Ruzgas, Tautgirdas LU (2004) In Bioelectrochemistry 63(1-2). p.277-280
Abstract
The reliable detection of a superoxide anion radical O-2(.-) - is complicated by its spontaneous dismutation reaction to H2O2 at acidic pHs. To simultaneously detect both O-2(.-) and H2O2 produced in the course of its spontaneous dismutation, an electrochemical two-electrode system based on cytochrome c (cyt c) and recombinant horseradish peroxidase (rHRP) was applied. Therewith, a limited applicability of the cyt c system for the reliable monitoring of O-2(.-) in acidic and neutral solutions was shown. It was demonstrated that both the reaction of O-2(.-) dismutation to H2O2 and the reaction between the formed H2O2 and O-2(.-) chemically decrease the amount of the initially present O-2(.-) decreasing the sensitivity and reliability of the... (More)
The reliable detection of a superoxide anion radical O-2(.-) - is complicated by its spontaneous dismutation reaction to H2O2 at acidic pHs. To simultaneously detect both O-2(.-) and H2O2 produced in the course of its spontaneous dismutation, an electrochemical two-electrode system based on cytochrome c (cyt c) and recombinant horseradish peroxidase (rHRP) was applied. Therewith, a limited applicability of the cyt c system for the reliable monitoring of O-2(.-) in acidic and neutral solutions was shown. It was demonstrated that both the reaction of O-2(.-) dismutation to H2O2 and the reaction between the formed H2O2 and O-2(.-) chemically decrease the amount of the initially present O-2(.-) decreasing the sensitivity and reliability of the electrochemical detection at acidic pH. However, by appropriately varying solution pH, the concentration of O-2(.-) - initially injected in the system can be estimated from the analysis of calibration curves for H2O2 obtained with highly sensitive rHRP-modified electrode system at pH 6.0 and 7.0. (C) 2004 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bi-electrode system, Recombinant horseradish peroxidase, Cytochrome c, Hydrogen peroxide, Superoxide radical
in
Bioelectrochemistry
volume
63
issue
1-2
pages
277 - 280
publisher
Elsevier
external identifiers
  • wos:000221786900050
  • pmid:15110287
  • scopus:2042456719
ISSN
1878-562X
DOI
10.1016/j.bioelechem.2003.09.026
language
English
LU publication?
yes
id
a874e76e-3f7d-4ee2-8739-5cbdfe327fd9 (old id 138522)
date added to LUP
2007-06-27 15:37:21
date last changed
2017-01-01 04:31:34
@article{a874e76e-3f7d-4ee2-8739-5cbdfe327fd9,
  abstract     = {The reliable detection of a superoxide anion radical O-2(.-) - is complicated by its spontaneous dismutation reaction to H2O2 at acidic pHs. To simultaneously detect both O-2(.-) and H2O2 produced in the course of its spontaneous dismutation, an electrochemical two-electrode system based on cytochrome c (cyt c) and recombinant horseradish peroxidase (rHRP) was applied. Therewith, a limited applicability of the cyt c system for the reliable monitoring of O-2(.-) in acidic and neutral solutions was shown. It was demonstrated that both the reaction of O-2(.-) dismutation to H2O2 and the reaction between the formed H2O2 and O-2(.-) chemically decrease the amount of the initially present O-2(.-) decreasing the sensitivity and reliability of the electrochemical detection at acidic pH. However, by appropriately varying solution pH, the concentration of O-2(.-) - initially injected in the system can be estimated from the analysis of calibration curves for H2O2 obtained with highly sensitive rHRP-modified electrode system at pH 6.0 and 7.0. (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Shipovskov, Stepan and Ferapontova, Elena and Gazaryan, I and Ruzgas, Tautgirdas},
  issn         = {1878-562X},
  keyword      = {Bi-electrode system,Recombinant horseradish peroxidase,Cytochrome c,Hydrogen peroxide,Superoxide radical},
  language     = {eng},
  number       = {1-2},
  pages        = {277--280},
  publisher    = {Elsevier},
  series       = {Bioelectrochemistry},
  title        = {Recombinant horseradish peroxidase- and cytochrome c-based two-electrode system for detection of superoxide radicals},
  url          = {http://dx.doi.org/10.1016/j.bioelechem.2003.09.026},
  volume       = {63},
  year         = {2004},
}