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Controlled fed-batch fermentations of dilute-acid hydrolysate in pilot development unit scale

Rudolf, Andreas LU ; Galbe, Mats LU and Lidén, Gunnar LU (2004) In Applied Biochemistry and Biotechnology 114(1-3). p.601-617
Abstract
Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented for use in pilot development unit (PDU) scale. The rate of total gas formation, measured with a mass flow meter, was used as input variable in the control algorithm. The feed rate in the PDU-scale experiments could be properly controlled based on the gas evolution from the reactor. In fed-batch experiments utilizing TMB 3000, an inhibitor-tolerant strain of Saccharomyces cerevisiae, close to 100% of the hexoses in the... (More)
Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented for use in pilot development unit (PDU) scale. The rate of total gas formation, measured with a mass flow meter, was used as input variable in the control algorithm. The feed rate in the PDU-scale experiments could be properly controlled based on the gas evolution from the reactor. In fed-batch experiments utilizing TMB 3000, an inhibitor-tolerant strain of Saccharomyces cerevisiae, close to 100% of the hexoses in the hydrolysate was converted. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Biochemistry and Biotechnology
volume
114
issue
1-3
pages
601 - 617
publisher
Humana Press
external identifiers
  • wos:000221186200050
  • scopus:11244315135
ISSN
1559-0291
DOI
10.1385/ABAB:114:1-3:601
language
English
LU publication?
yes
id
51d38725-f2f9-4c60-ae40-ed953f6d56dd (old id 138861)
date added to LUP
2007-06-25 15:43:45
date last changed
2017-07-23 03:34:35
@article{51d38725-f2f9-4c60-ae40-ed953f6d56dd,
  abstract     = {Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented for use in pilot development unit (PDU) scale. The rate of total gas formation, measured with a mass flow meter, was used as input variable in the control algorithm. The feed rate in the PDU-scale experiments could be properly controlled based on the gas evolution from the reactor. In fed-batch experiments utilizing TMB 3000, an inhibitor-tolerant strain of Saccharomyces cerevisiae, close to 100% of the hexoses in the hydrolysate was converted.},
  author       = {Rudolf, Andreas and Galbe, Mats and Lidén, Gunnar},
  issn         = {1559-0291},
  language     = {eng},
  number       = {1-3},
  pages        = {601--617},
  publisher    = {Humana Press},
  series       = {Applied Biochemistry and Biotechnology},
  title        = {Controlled fed-batch fermentations of dilute-acid hydrolysate in pilot development unit scale},
  url          = {http://dx.doi.org/10.1385/ABAB:114:1-3:601},
  volume       = {114},
  year         = {2004},
}