Advanced

Differential Utilization of Carbon Substrates by Bacteria and Fungi in Tundra Soil

Rinnan, Riikka LU and Bååth, Erland LU (2009) In Applied and Environmental Microbiology 75(11). p.3611-3620
Abstract
Little is known about the contribution of bacteria and fungi to decomposition of different carbon compounds in arctic soils, which are an important carbon store and possibly vulnerable to climate warming. Soil samples from a subarctic tundra heath were incubated with C-13-labeled glucose, acetic acid, glycine, starch, and vanillin, and the incorporation of C-13 into different phospholipid fatty acids (PLFA; indicative of growth) and neutral lipid fatty acids (NLFA; indicative of fungal storage) was measured after 1 and 7 days. The use of C-13-labeled substrates allowed the addition of substrates at concentrations low enough not to affect the total amount of PLFA. The label of glucose and acetic acid was rapidly incorporated into the PLFA... (More)
Little is known about the contribution of bacteria and fungi to decomposition of different carbon compounds in arctic soils, which are an important carbon store and possibly vulnerable to climate warming. Soil samples from a subarctic tundra heath were incubated with C-13-labeled glucose, acetic acid, glycine, starch, and vanillin, and the incorporation of C-13 into different phospholipid fatty acids (PLFA; indicative of growth) and neutral lipid fatty acids (NLFA; indicative of fungal storage) was measured after 1 and 7 days. The use of C-13-labeled substrates allowed the addition of substrates at concentrations low enough not to affect the total amount of PLFA. The label of glucose and acetic acid was rapidly incorporated into the PLFA in a pattern largely corresponding to the fatty acid concentration profile, while glycine and especially starch were mainly taken up by bacteria and not fungi, showing that different groups of the microbial community were responsible for substrate utilization. The C-13-incorporation from the complex substrates (starch and vanillin) increased over time. There was significant allocation of C-13 into the fungal NLFA, except for starch. For glucose, acetic acid, and glycine, the allocation decreased over time, indicating use of the storage products, whereas for vanillin incorporation into fungal NLFA increased during the incubation. In addition to providing information on functioning of the microbial communities in an arctic soil, our study showed that the combination of PLFA and NLFA analyses yields additional information on the dynamics of substrate degradation. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
75
issue
11
pages
3611 - 3620
publisher
American Society for Microbiology
external identifiers
  • scopus:66249135970
  • wos:000266345800029
ISSN
0099-2240
DOI
10.1128/AEM.02865-08
language
English
LU publication?
yes
id
3821e95b-cd67-4d8f-8a05-37e8065285fb (old id 1392170)
date added to LUP
2009-07-02 16:11:10
date last changed
2017-12-10 03:38:13
@article{3821e95b-cd67-4d8f-8a05-37e8065285fb,
  abstract     = {Little is known about the contribution of bacteria and fungi to decomposition of different carbon compounds in arctic soils, which are an important carbon store and possibly vulnerable to climate warming. Soil samples from a subarctic tundra heath were incubated with C-13-labeled glucose, acetic acid, glycine, starch, and vanillin, and the incorporation of C-13 into different phospholipid fatty acids (PLFA; indicative of growth) and neutral lipid fatty acids (NLFA; indicative of fungal storage) was measured after 1 and 7 days. The use of C-13-labeled substrates allowed the addition of substrates at concentrations low enough not to affect the total amount of PLFA. The label of glucose and acetic acid was rapidly incorporated into the PLFA in a pattern largely corresponding to the fatty acid concentration profile, while glycine and especially starch were mainly taken up by bacteria and not fungi, showing that different groups of the microbial community were responsible for substrate utilization. The C-13-incorporation from the complex substrates (starch and vanillin) increased over time. There was significant allocation of C-13 into the fungal NLFA, except for starch. For glucose, acetic acid, and glycine, the allocation decreased over time, indicating use of the storage products, whereas for vanillin incorporation into fungal NLFA increased during the incubation. In addition to providing information on functioning of the microbial communities in an arctic soil, our study showed that the combination of PLFA and NLFA analyses yields additional information on the dynamics of substrate degradation.},
  author       = {Rinnan, Riikka and Bååth, Erland},
  issn         = {0099-2240},
  language     = {eng},
  number       = {11},
  pages        = {3611--3620},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Differential Utilization of Carbon Substrates by Bacteria and Fungi in Tundra Soil},
  url          = {http://dx.doi.org/10.1128/AEM.02865-08},
  volume       = {75},
  year         = {2009},
}