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Functional Studies of Telomere-Binding Proteins in Saccharomyces castellii

Rhodin, Jenny LU (2009)
Abstract
Telomeres are the ends of linear eukaryotic chromosomes. The telomeres are essential for solving the end replication problem and they stabilize the genome by protection from degradation. The telomeres also protect the chromosomal ends from by being recognized as DNA breaks by the repair machinery, which could lead to end-to-end fusions. Dysfunctional telomeres are implicated in many complex cellular processes like replicative senescence and tumorgenesis. This clearly indicates the importance of maintenance and length regulation of telomeres.

The telomeres are built up by TG-rich tandem repeats and specific proteins binding these repeats. After replication the telomeres can be elongated by the reverse transcriptase, telomerase.... (More)
Telomeres are the ends of linear eukaryotic chromosomes. The telomeres are essential for solving the end replication problem and they stabilize the genome by protection from degradation. The telomeres also protect the chromosomal ends from by being recognized as DNA breaks by the repair machinery, which could lead to end-to-end fusions. Dysfunctional telomeres are implicated in many complex cellular processes like replicative senescence and tumorgenesis. This clearly indicates the importance of maintenance and length regulation of telomeres.

The telomeres are built up by TG-rich tandem repeats and specific proteins binding these repeats. After replication the telomeres can be elongated by the reverse transcriptase, telomerase. In this work an in vitro system that mimics the natural telomere end has been developed. This can be used to investigate the molecular mechanisms involved in the regulation of the telomere length.

I have characterized two telomere binding proteins, Rap1 and Cdc13, from the yeast species Saccharomyces castellii. This yeast has properties beneficial for telomere research, like regularly repeated telomeric DNA. The obtained information regarding the binding specificities of S. castellii Rap1 (scasRap1) and S. castellii Cdc13 (scasCdc13) has been used to study the protein interactions. The ability of scasCdc13 to affect telomerase dependent telomere elongation has been investigated as well. Moreover we have shown that these two different classes of telomere-binding proteins act together in order to conserve a telomeric core sequence during evolution. (Less)
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author
supervisor
opponent
  • PhD, DSc Tomaska, Lubomir, Department of Genetics, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Rap1, DNA-binding domain, telomere evolution, Cdc13, telomerase, single-stranded overhang, telomere, yeast, Naumovia castellii, Saccharomyces castellii
pages
188 pages
defense location
Biology Lecture Hall, Biology Building, Sölvegatan 35, 223 62 Lund
defense date
2009-06-02 09:30
ISBN
978-91-85067-55-8
language
English
LU publication?
yes
id
b78f2df7-5c05-46cc-bc5c-4299a3258bd7 (old id 1392755)
date added to LUP
2009-05-07 11:44:07
date last changed
2016-09-19 08:45:18
@phdthesis{b78f2df7-5c05-46cc-bc5c-4299a3258bd7,
  abstract     = {Telomeres are the ends of linear eukaryotic chromosomes. The telomeres are essential for solving the end replication problem and they stabilize the genome by protection from degradation. The telomeres also protect the chromosomal ends from by being recognized as DNA breaks by the repair machinery, which could lead to end-to-end fusions. Dysfunctional telomeres are implicated in many complex cellular processes like replicative senescence and tumorgenesis. This clearly indicates the importance of maintenance and length regulation of telomeres. <br/><br>
	The telomeres are built up by TG-rich tandem repeats and specific proteins binding these repeats. After replication the telomeres can be elongated by the reverse transcriptase, telomerase. In this work an in vitro system that mimics the natural telomere end has been developed. This can be used to investigate the molecular mechanisms involved in the regulation of the telomere length.<br/><br>
	I have characterized two telomere binding proteins, Rap1 and Cdc13, from the yeast species Saccharomyces castellii. This yeast has properties beneficial for telomere research, like regularly repeated telomeric DNA. The obtained information regarding the binding specificities of S. castellii Rap1 (scasRap1) and S. castellii Cdc13 (scasCdc13) has been used to study the protein interactions. The ability of scasCdc13 to affect telomerase dependent telomere elongation has been investigated as well. Moreover we have shown that these two different classes of telomere-binding proteins act together in order to conserve a telomeric core sequence during evolution.},
  author       = {Rhodin, Jenny},
  isbn         = {978-91-85067-55-8},
  keyword      = {Rap1,DNA-binding domain,telomere evolution,Cdc13,telomerase,single-stranded overhang,telomere,yeast,Naumovia castellii,Saccharomyces castellii},
  language     = {eng},
  pages        = {188},
  school       = {Lund University},
  title        = {Functional Studies of Telomere-Binding Proteins in Saccharomyces castellii},
  year         = {2009},
}