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Human SGBS Cells - a Unique Tool for Studies of Human Fat Cell Biology

Fischer-Posovszky, Pamela; Newell, Felicity S.; Wabitsch, Martin and Tornqvist, Hans LU (2008) In Obesity Facts 1(4). p.184-189
Abstract
The human Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte cell strain provides a unique and useful tool for studies of human adipocyte biology. The cells originate from an adipose tissue specimen of a patient with SGBS. They are neither transformed nor immortalized, and provide an almost unlimited source due to their ability to proliferate for up to 50 generations with retained capacity for adipogenic differentiation. So far, the cells have been used for a number of studies on adipose differentiation, adipocyte glucose uptake, lipolysis, apoptosis, regulation of expression of adipokines, and protein translocation. The cells are efficiently differentiated in the presence of PPAR gamma agonists and in the absence of serum and albumin.... (More)
The human Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte cell strain provides a unique and useful tool for studies of human adipocyte biology. The cells originate from an adipose tissue specimen of a patient with SGBS. They are neither transformed nor immortalized, and provide an almost unlimited source due to their ability to proliferate for up to 50 generations with retained capacity for adipogenic differentiation. So far, the cells have been used for a number of studies on adipose differentiation, adipocyte glucose uptake, lipolysis, apoptosis, regulation of expression of adipokines, and protein translocation. The cells are efficiently differentiated in the presence of PPAR gamma agonists and in the absence of serum and albumin. SGBS adipocytes respond to insulin stimulation by increasing glucose uptake several-fold (EC50 approximately 100 pmol/l), and by very effectively inhibiting (IC50 approximately 10 pmol/l) catecholamine-stimulated lipolysis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adipocyte, Fat cell, SGBS
in
Obesity Facts
volume
1
issue
4
pages
184 - 189
publisher
Karger
external identifiers
  • wos:000265056400003
  • scopus:77954302595
ISSN
1662-4033
DOI
10.1159/000145784
language
English
LU publication?
yes
id
dd98f675-2f81-47fe-9a61-856d924ae865 (old id 1399267)
date added to LUP
2009-05-28 13:26:33
date last changed
2017-11-19 03:29:19
@article{dd98f675-2f81-47fe-9a61-856d924ae865,
  abstract     = {The human Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte cell strain provides a unique and useful tool for studies of human adipocyte biology. The cells originate from an adipose tissue specimen of a patient with SGBS. They are neither transformed nor immortalized, and provide an almost unlimited source due to their ability to proliferate for up to 50 generations with retained capacity for adipogenic differentiation. So far, the cells have been used for a number of studies on adipose differentiation, adipocyte glucose uptake, lipolysis, apoptosis, regulation of expression of adipokines, and protein translocation. The cells are efficiently differentiated in the presence of PPAR gamma agonists and in the absence of serum and albumin. SGBS adipocytes respond to insulin stimulation by increasing glucose uptake several-fold (EC50 approximately 100 pmol/l), and by very effectively inhibiting (IC50 approximately 10 pmol/l) catecholamine-stimulated lipolysis.},
  author       = {Fischer-Posovszky, Pamela and Newell, Felicity S. and Wabitsch, Martin and Tornqvist, Hans},
  issn         = {1662-4033},
  keyword      = {Adipocyte,Fat cell,SGBS},
  language     = {eng},
  number       = {4},
  pages        = {184--189},
  publisher    = {Karger},
  series       = {Obesity Facts},
  title        = {Human SGBS Cells - a Unique Tool for Studies of Human Fat Cell Biology},
  url          = {http://dx.doi.org/10.1159/000145784},
  volume       = {1},
  year         = {2008},
}