Human SGBS Cells - a Unique Tool for Studies of Human Fat Cell Biology
(2008) In Obesity Facts 1(4). p.184-189- Abstract
- The human Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte cell strain provides a unique and useful tool for studies of human adipocyte biology. The cells originate from an adipose tissue specimen of a patient with SGBS. They are neither transformed nor immortalized, and provide an almost unlimited source due to their ability to proliferate for up to 50 generations with retained capacity for adipogenic differentiation. So far, the cells have been used for a number of studies on adipose differentiation, adipocyte glucose uptake, lipolysis, apoptosis, regulation of expression of adipokines, and protein translocation. The cells are efficiently differentiated in the presence of PPAR gamma agonists and in the absence of serum and albumin.... (More)
- The human Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte cell strain provides a unique and useful tool for studies of human adipocyte biology. The cells originate from an adipose tissue specimen of a patient with SGBS. They are neither transformed nor immortalized, and provide an almost unlimited source due to their ability to proliferate for up to 50 generations with retained capacity for adipogenic differentiation. So far, the cells have been used for a number of studies on adipose differentiation, adipocyte glucose uptake, lipolysis, apoptosis, regulation of expression of adipokines, and protein translocation. The cells are efficiently differentiated in the presence of PPAR gamma agonists and in the absence of serum and albumin. SGBS adipocytes respond to insulin stimulation by increasing glucose uptake several-fold (EC50 approximately 100 pmol/l), and by very effectively inhibiting (IC50 approximately 10 pmol/l) catecholamine-stimulated lipolysis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1399267
- author
- Fischer-Posovszky, Pamela ; Newell, Felicity S. ; Wabitsch, Martin and Tornqvist, Hans LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adipocyte, Fat cell, SGBS
- in
- Obesity Facts
- volume
- 1
- issue
- 4
- pages
- 184 - 189
- publisher
- Karger
- external identifiers
-
- wos:000265056400003
- scopus:77954302595
- pmid:20054179
- ISSN
- 1662-4033
- DOI
- 10.1159/000145784
- language
- English
- LU publication?
- yes
- id
- dd98f675-2f81-47fe-9a61-856d924ae865 (old id 1399267)
- date added to LUP
- 2016-04-01 11:51:44
- date last changed
- 2024-05-07 17:58:22
@article{dd98f675-2f81-47fe-9a61-856d924ae865, abstract = {{The human Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte cell strain provides a unique and useful tool for studies of human adipocyte biology. The cells originate from an adipose tissue specimen of a patient with SGBS. They are neither transformed nor immortalized, and provide an almost unlimited source due to their ability to proliferate for up to 50 generations with retained capacity for adipogenic differentiation. So far, the cells have been used for a number of studies on adipose differentiation, adipocyte glucose uptake, lipolysis, apoptosis, regulation of expression of adipokines, and protein translocation. The cells are efficiently differentiated in the presence of PPAR gamma agonists and in the absence of serum and albumin. SGBS adipocytes respond to insulin stimulation by increasing glucose uptake several-fold (EC50 approximately 100 pmol/l), and by very effectively inhibiting (IC50 approximately 10 pmol/l) catecholamine-stimulated lipolysis.}}, author = {{Fischer-Posovszky, Pamela and Newell, Felicity S. and Wabitsch, Martin and Tornqvist, Hans}}, issn = {{1662-4033}}, keywords = {{Adipocyte; Fat cell; SGBS}}, language = {{eng}}, number = {{4}}, pages = {{184--189}}, publisher = {{Karger}}, series = {{Obesity Facts}}, title = {{Human SGBS Cells - a Unique Tool for Studies of Human Fat Cell Biology}}, url = {{http://dx.doi.org/10.1159/000145784}}, doi = {{10.1159/000145784}}, volume = {{1}}, year = {{2008}}, }