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Temperature Induced DNA Compaction in a Nonionic Lamellar Phase

Gonzalez Perez, Alfredo LU ; Bulut, Sanja LU ; Olsson, Ulf LU and Lindman, Björn LU (2008) 9th International Conference on Colloid Chemistry, 1997 In Colloids for Nano- and Biotechnology (Progress in Colloid and Polymer Science) 135. p.174-180
Abstract
A nonionic lamellar phase was prepared using C10E3 in buffer solution at pH = 7.6. A suitable T4DNA concentration around 5 wt % was immobilized in a lamellar phase with 40 wt % C10E3. The mixed system was investigated at two temperatures, 25 and 5 degrees C by using cryo-fracture TEM direct imaging, fluorescence microscopy and small-angle X-ray scattering. The surprising results where obtained showing that the DNA conformation can be tuned to compacted and extended state at 25 and 5 degrees C, respectively. Additionally, DNA is aligned with a preferential orientation in the direction of the flow by simply injecting the sample in a capillary.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
compaction, surfactant, DNA, decompaction, Mesh phase, Nonionic, C10E3, lamellar phase
in
Colloids for Nano- and Biotechnology (Progress in Colloid and Polymer Science)
volume
135
pages
174 - 180
publisher
Springer
conference name
9th International Conference on Colloid Chemistry, 1997
external identifiers
  • wos:000264002200025
  • scopus:62449186768
ISSN
0340-255X
ISBN
978-3-540-85133-2
DOI
10.1007/2882_2008_106
language
English
LU publication?
yes
id
938e24bc-9a51-435f-aeb1-04b3f934f6fd (old id 1399355)
date added to LUP
2009-05-28 13:10:31
date last changed
2017-01-01 06:32:32
@inproceedings{938e24bc-9a51-435f-aeb1-04b3f934f6fd,
  abstract     = {A nonionic lamellar phase was prepared using C10E3 in buffer solution at pH = 7.6. A suitable T4DNA concentration around 5 wt % was immobilized in a lamellar phase with 40 wt % C10E3. The mixed system was investigated at two temperatures, 25 and 5 degrees C by using cryo-fracture TEM direct imaging, fluorescence microscopy and small-angle X-ray scattering. The surprising results where obtained showing that the DNA conformation can be tuned to compacted and extended state at 25 and 5 degrees C, respectively. Additionally, DNA is aligned with a preferential orientation in the direction of the flow by simply injecting the sample in a capillary.},
  author       = {Gonzalez Perez, Alfredo and Bulut, Sanja and Olsson, Ulf and Lindman, Björn},
  booktitle    = {Colloids for Nano- and Biotechnology (Progress in Colloid and Polymer Science)},
  isbn         = {978-3-540-85133-2},
  issn         = {0340-255X},
  keyword      = {compaction,surfactant,DNA,decompaction,Mesh phase,Nonionic,C10E3,lamellar phase},
  language     = {eng},
  pages        = {174--180},
  publisher    = {Springer},
  title        = {Temperature Induced DNA Compaction in a Nonionic Lamellar Phase},
  url          = {http://dx.doi.org/10.1007/2882_2008_106},
  volume       = {135},
  year         = {2008},
}