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Copper induced root growth inhibition of Allium cepa var. Agrogarum L. involves disturbances in cell division and DNA damage

Björn, Lars Olof LU ; Qin, Rong; Wang, Congyue; Chen, Da and Li, Shaoshan (2015) In Environmental Toxicology and Chemistry 34(5). p.1045-1055
Abstract
Copper (Cu) is considered to be an indispensable microelement for plants. Excessive Cu, however, is toxic and disturbs several
processes in the plant. The present study addressed the effects of ionic Cu (2.0 mMand 8.0mM) on mitosis, the microtubule cytoskeleton,
and DNA in root tip cells of Allium cepa var. agrogarum L. to better understand Cu toxicity on plant root systems. The results indicated that Cu accumulated in roots and that root growth was inhibited dramatically in Cu treatment groups. Chromosomal aberrations (for example, C-mitosis, chromosome bridges, chromosome stickiness, and micronucleus) were observed, and the mitotic index decreased during Cu treatments at different concentrations. Microtubules were one of the... (More)
Copper (Cu) is considered to be an indispensable microelement for plants. Excessive Cu, however, is toxic and disturbs several
processes in the plant. The present study addressed the effects of ionic Cu (2.0 mMand 8.0mM) on mitosis, the microtubule cytoskeleton,
and DNA in root tip cells of Allium cepa var. agrogarum L. to better understand Cu toxicity on plant root systems. The results indicated that Cu accumulated in roots and that root growth was inhibited dramatically in Cu treatment groups. Chromosomal aberrations (for example, C-mitosis, chromosome bridges, chromosome stickiness, and micronucleus) were observed, and the mitotic index decreased during Cu treatments at different concentrations. Microtubules were one of the target sites of Cu toxicity in root tip meristematic cells, and Cu exposure substantially impaired microtubule arrangements. The content of a-tubulin decreased following 36 h of exposure to 2.0mM or 8.0mM of Cu in comparison with the control group. Copper increased DNA damage and suppressed cell cycle progression. The above toxic effects became more serious with increasing Cu concentration and prolonged exposure time. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Copper, Mitosis, Microtubule, DNA damage, DNA syntyhesis
in
Environmental Toxicology and Chemistry
volume
34
issue
5
pages
11 pages
publisher
SETAC and Alliance Communications Group
external identifiers
  • scopus:84928138895
ISSN
0730-7268
DOI
10.1002/etc.2884
language
English
LU publication?
yes
id
13be04ce-d855-4f3e-afeb-9ab311ebeff2
date added to LUP
2016-06-06 16:39:00
date last changed
2017-03-26 04:43:02
@article{13be04ce-d855-4f3e-afeb-9ab311ebeff2,
  abstract     = {Copper (Cu) is considered to be an indispensable microelement for plants. Excessive Cu, however, is toxic and disturbs several<br/>processes in the plant. The present study addressed the effects of ionic Cu (2.0 mMand 8.0mM) on mitosis, the microtubule cytoskeleton,<br/>and DNA in root tip cells of Allium cepa var. agrogarum L. to better understand Cu toxicity on plant root systems. The results indicated that Cu accumulated in roots and that root growth was inhibited dramatically in Cu treatment groups. Chromosomal aberrations (for example, C-mitosis, chromosome bridges, chromosome stickiness, and micronucleus) were observed, and the mitotic index decreased during Cu treatments at different concentrations. Microtubules were one of the target sites of Cu toxicity in root tip meristematic cells, and Cu exposure substantially impaired microtubule arrangements. The content of a-tubulin decreased following 36 h of exposure to 2.0mM or 8.0mM of Cu in comparison with the control group. Copper increased DNA damage and suppressed cell cycle progression. The above toxic effects became more serious with increasing Cu concentration and prolonged exposure time.},
  author       = {Björn, Lars Olof and Qin, Rong and Wang, Congyue and Chen, Da and Li, Shaoshan},
  issn         = {0730-7268},
  keyword      = {Copper,Mitosis,Microtubule,DNA damage,DNA syntyhesis},
  language     = {eng},
  month        = {04},
  number       = {5},
  pages        = {1045--1055},
  publisher    = {SETAC and Alliance Communications Group},
  series       = {Environmental Toxicology and Chemistry},
  title        = {Copper induced root growth inhibition of Allium cepa var. Agrogarum L. involves disturbances in cell division and DNA damage},
  url          = {http://dx.doi.org/10.1002/etc.2884},
  volume       = {34},
  year         = {2015},
}