Copper induced root growth inhibition of Allium cepa var. Agrogarum L. involves disturbances in cell division and DNA damage
Qin, Rong ; Wang, Congyue ; Chen, Da ; Björn, Lars Olof LU
and Li, Shaoshan
(2015)
In Environmental Toxicology and Chemistry
34(5).
p.1045-1055
- Abstract
- Copper (Cu) is considered to be an indispensable microelement for plants. Excessive Cu, however, is toxic and disturbs several
processes in the plant. The present study addressed the effects of ionic Cu (2.0 mMand 8.0mM) on mitosis, the microtubule cytoskeleton,
and DNA in root tip cells of Allium cepa var. agrogarum L. to better understand Cu toxicity on plant root systems. The results indicated that Cu accumulated in roots and that root growth was inhibited dramatically in Cu treatment groups. Chromosomal aberrations (for example, C-mitosis, chromosome bridges, chromosome stickiness, and micronucleus) were observed, and the mitotic index decreased during Cu treatments at different concentrations. Microtubules were one of the... (More) - Copper (Cu) is considered to be an indispensable microelement for plants. Excessive Cu, however, is toxic and disturbs several
processes in the plant. The present study addressed the effects of ionic Cu (2.0 mMand 8.0mM) on mitosis, the microtubule cytoskeleton,
and DNA in root tip cells of Allium cepa var. agrogarum L. to better understand Cu toxicity on plant root systems. The results indicated that Cu accumulated in roots and that root growth was inhibited dramatically in Cu treatment groups. Chromosomal aberrations (for example, C-mitosis, chromosome bridges, chromosome stickiness, and micronucleus) were observed, and the mitotic index decreased during Cu treatments at different concentrations. Microtubules were one of the target sites of Cu toxicity in root tip meristematic cells, and Cu exposure substantially impaired microtubule arrangements. The content of a-tubulin decreased following 36 h of exposure to 2.0mM or 8.0mM of Cu in comparison with the control group. Copper increased DNA damage and suppressed cell cycle progression. The above toxic effects became more serious with increasing Cu concentration and prolonged exposure time. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/13be04ce-d855-4f3e-afeb-9ab311ebeff2
- author
- Qin, Rong
; Wang, Congyue
; Chen, Da
; Björn, Lars Olof
LU
and Li, Shaoshan
- organization
- publishing date
- 2015-04-21
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Copper, Mitosis, Microtubule, DNA damage, DNA syntyhesis
- in
- Environmental Toxicology and Chemistry
- volume
- 34
- issue
- 5
- pages
- 11 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:84928138895
- pmid:25639377
- ISSN
- 0730-7268
- DOI
- 10.1002/etc.2884
- language
- English
- LU publication?
- yes
- id
- 13be04ce-d855-4f3e-afeb-9ab311ebeff2
- date added to LUP
- 2016-06-06 16:39:00
- date last changed
- 2023-08-21 15:19:32
@article{13be04ce-d855-4f3e-afeb-9ab311ebeff2,
abstract = {{Copper (Cu) is considered to be an indispensable microelement for plants. Excessive Cu, however, is toxic and disturbs several<br/>processes in the plant. The present study addressed the effects of ionic Cu (2.0 mMand 8.0mM) on mitosis, the microtubule cytoskeleton,<br/>and DNA in root tip cells of Allium cepa var. agrogarum L. to better understand Cu toxicity on plant root systems. The results indicated that Cu accumulated in roots and that root growth was inhibited dramatically in Cu treatment groups. Chromosomal aberrations (for example, C-mitosis, chromosome bridges, chromosome stickiness, and micronucleus) were observed, and the mitotic index decreased during Cu treatments at different concentrations. Microtubules were one of the target sites of Cu toxicity in root tip meristematic cells, and Cu exposure substantially impaired microtubule arrangements. The content of a-tubulin decreased following 36 h of exposure to 2.0mM or 8.0mM of Cu in comparison with the control group. Copper increased DNA damage and suppressed cell cycle progression. The above toxic effects became more serious with increasing Cu concentration and prolonged exposure time.}},
author = {{Qin, Rong and Wang, Congyue and Chen, Da and Björn, Lars Olof and Li, Shaoshan}},
issn = {{0730-7268}},
keywords = {{Copper; Mitosis; Microtubule; DNA damage; DNA syntyhesis}},
language = {{eng}},
month = {{04}},
number = {{5}},
pages = {{1045--1055}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Environmental Toxicology and Chemistry}},
title = {{Copper induced root growth inhibition of Allium cepa var. Agrogarum L. involves disturbances in cell division and DNA damage}},
url = {{http://dx.doi.org/10.1002/etc.2884}},
doi = {{10.1002/etc.2884}},
volume = {{34}},
year = {{2015}},
}