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Magnetic permeability based diagnostic test for the determination of the canine C-reactive protein concentration in undiluted whole blood

Ibraimi, Filiz LU ; Kriz, Kirstin LU ; Merin, Henrik and Kriz, Dario (2009) 7th International Conference on Scientific and Clinical Applications of Magnetic Carriers In Journal Of Magnetism And Magnetic Materials 321(10). p.1632-1634
Abstract
We describe an one-step 11-min magnetic permeability based two-site immunoassay for C-reactive protein (CRP) utilizing polyclonal anti-canine CRP antibody conjugated dextran iron oxide nanoparticles (79 nm) as superparamagnetic labels and polyclonal anti-canine CRP conjugated silica microparticles (15 to 40 mu m) as carriers. An inductance based magnetic permeability reader was used to detect the target analyte, CRP, in 10 mu L whole blood samples, by measuring the magnetic permeability increase of the silica microparticle sediment due to immuno complex superparamagnetic nanoparticles. Measurements on standards showed a linear response between 0 and 17.5 mg/L CRP. Measurements performed on 16 whole blood samples from mixed breeds showed... (More)
We describe an one-step 11-min magnetic permeability based two-site immunoassay for C-reactive protein (CRP) utilizing polyclonal anti-canine CRP antibody conjugated dextran iron oxide nanoparticles (79 nm) as superparamagnetic labels and polyclonal anti-canine CRP conjugated silica microparticles (15 to 40 mu m) as carriers. An inductance based magnetic permeability reader was used to detect the target analyte, CRP, in 10 mu L whole blood samples, by measuring the magnetic permeability increase of the silica microparticle sediment due to immuno complex superparamagnetic nanoparticles. Measurements on standards showed a linear response between 0 and 17.5 mg/L CRP. Measurements performed on 16 whole blood samples from mixed breeds showed good correlation with a commercially available ELISA assay. (C) 2009 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Veterinary clinics, Diagnostic test, Two-site immunoassay, nanoparticles, Superparamagnetic, Canine C-reactive protein, Magnetic permeability
in
Journal Of Magnetism And Magnetic Materials
volume
321
issue
10
pages
1632 - 1634
publisher
Elsevier
conference name
7th International Conference on Scientific and Clinical Applications of Magnetic Carriers
external identifiers
  • wos:000265278000068
  • scopus:64249131409
ISSN
0304-8853
DOI
10.1016/j.jmmm.2009.02.102
language
English
LU publication?
yes
id
3b825e38-895c-4e04-9c37-02614972db6b (old id 1400211)
date added to LUP
2009-06-15 14:39:46
date last changed
2017-04-16 03:58:17
@inproceedings{3b825e38-895c-4e04-9c37-02614972db6b,
  abstract     = {We describe an one-step 11-min magnetic permeability based two-site immunoassay for C-reactive protein (CRP) utilizing polyclonal anti-canine CRP antibody conjugated dextran iron oxide nanoparticles (79 nm) as superparamagnetic labels and polyclonal anti-canine CRP conjugated silica microparticles (15 to 40 mu m) as carriers. An inductance based magnetic permeability reader was used to detect the target analyte, CRP, in 10 mu L whole blood samples, by measuring the magnetic permeability increase of the silica microparticle sediment due to immuno complex superparamagnetic nanoparticles. Measurements on standards showed a linear response between 0 and 17.5 mg/L CRP. Measurements performed on 16 whole blood samples from mixed breeds showed good correlation with a commercially available ELISA assay. (C) 2009 Elsevier B.V. All rights reserved.},
  author       = {Ibraimi, Filiz and Kriz, Kirstin and Merin, Henrik and Kriz, Dario},
  booktitle    = {Journal Of Magnetism And Magnetic Materials},
  issn         = {0304-8853},
  keyword      = {Veterinary clinics,Diagnostic test,Two-site immunoassay,nanoparticles,Superparamagnetic,Canine C-reactive protein,Magnetic permeability},
  language     = {eng},
  number       = {10},
  pages        = {1632--1634},
  publisher    = {Elsevier},
  title        = {Magnetic permeability based diagnostic test for the determination of the canine C-reactive protein concentration in undiluted whole blood},
  url          = {http://dx.doi.org/10.1016/j.jmmm.2009.02.102},
  volume       = {321},
  year         = {2009},
}