Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine
(2009) In Biochemical and Biophysical Research Communications 382(2). p.430-433- Abstract
- Drosophila melanogaster multisubstrate deoxyribonucleoside kinase (Dm-dNK) can additionally sensitize human cancer cell lines towards the anti-cancer drug gemcitabine. We show that this property is based on the Dm-dNK ability to efficiently phosphorylate gemcitabine. The 2.2 angstrom resolution structure of DmdNK in complex with gemcitabine shows that the residues Tyr70 and Arg105 play a crucial role in the firm positioning of gemcitabine by extra interactions made by the fluoride atoms. This explains why gemcitabine is a good substrate for Dm-dNK(. (C) 2009 Elsevier Inc. All rights reserved.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1400387
- author
- Knecht, Wolfgang LU ; Mikkelsen, Nils Egil ; Clausen, Anders Ranegaard LU ; Willer, Mette ; Eklund, Hans ; Gojkovic, Zoran and Piskur, Jure LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Structure-function relationship, Salvage pathway, Cancer, Gene-therapy, Nucleoside analogs, Deoxyribonucleoside kinase
- in
- Biochemical and Biophysical Research Communications
- volume
- 382
- issue
- 2
- pages
- 430 - 433
- publisher
- Elsevier
- external identifiers
-
- wos:000265279300039
- scopus:63349095879
- ISSN
- 1090-2104
- DOI
- 10.1016/j.bbrc.2009.03.041
- language
- English
- LU publication?
- yes
- id
- bf59b6d7-c510-41e2-9613-dee11d62665f (old id 1400387)
- date added to LUP
- 2016-04-01 13:37:34
- date last changed
- 2022-01-27 20:15:03
@article{bf59b6d7-c510-41e2-9613-dee11d62665f, abstract = {{Drosophila melanogaster multisubstrate deoxyribonucleoside kinase (Dm-dNK) can additionally sensitize human cancer cell lines towards the anti-cancer drug gemcitabine. We show that this property is based on the Dm-dNK ability to efficiently phosphorylate gemcitabine. The 2.2 angstrom resolution structure of DmdNK in complex with gemcitabine shows that the residues Tyr70 and Arg105 play a crucial role in the firm positioning of gemcitabine by extra interactions made by the fluoride atoms. This explains why gemcitabine is a good substrate for Dm-dNK(. (C) 2009 Elsevier Inc. All rights reserved.}}, author = {{Knecht, Wolfgang and Mikkelsen, Nils Egil and Clausen, Anders Ranegaard and Willer, Mette and Eklund, Hans and Gojkovic, Zoran and Piskur, Jure}}, issn = {{1090-2104}}, keywords = {{Structure-function relationship; Salvage pathway; Cancer; Gene-therapy; Nucleoside analogs; Deoxyribonucleoside kinase}}, language = {{eng}}, number = {{2}}, pages = {{430--433}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine}}, url = {{http://dx.doi.org/10.1016/j.bbrc.2009.03.041}}, doi = {{10.1016/j.bbrc.2009.03.041}}, volume = {{382}}, year = {{2009}}, }