Periplasmic c Cytochromes and Chlorate Reduction in Ideonella dechloratans
(2009) In Applied and Environmental Microbiology 75(8). p.2439-2445- Abstract
- The aim of this study was to clarify the pathway of electron transfer between the inner membrane components and the periplasmic chlorate reductase. Several soluble c-type cytochromes were found in the periplasm. The optical difference spectrum of dithionite-reduced periplasmic extract shows that at least one of these components is capable of acting as an electron donor to the enzyme chlorate reductase. The cytochromes were partially separated, and the fractions were analyzed by UV/visible spectroscopy to determine the ability of donating electrons to chlorate reductase. Our results show that one of the c cytochromes (6 kDa) is able to donate electrons, both to chlorate reductase and to the membrane-bound cytochrome c oxidase, whereas the... (More)
- The aim of this study was to clarify the pathway of electron transfer between the inner membrane components and the periplasmic chlorate reductase. Several soluble c-type cytochromes were found in the periplasm. The optical difference spectrum of dithionite-reduced periplasmic extract shows that at least one of these components is capable of acting as an electron donor to the enzyme chlorate reductase. The cytochromes were partially separated, and the fractions were analyzed by UV/visible spectroscopy to determine the ability of donating electrons to chlorate reductase. Our results show that one of the c cytochromes (6 kDa) is able to donate electrons, both to chlorate reductase and to the membrane-bound cytochrome c oxidase, whereas the roles of the remaining c cytochromes still remain to be elucidated. Peptide extracts of the c cytochromes were obtained by tryptic in-gel digestion for matrix-assisted laser desorption ionization -time of flight mass spectrometry analysis. Peptide sequences obtained indicate that the 6-kDa cytochrome c protein is similar to c cytochromes from the chlorate-reducing bacterium Dechloromonas aromatica. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1400783
- author
- Backlund, Anna Smedja ; Bohlin, Jan ; Gustavsson, Niklas LU and Nilsson, Thomas
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied and Environmental Microbiology
- volume
- 75
- issue
- 8
- pages
- 2439 - 2445
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000264936800024
- pmid:19233956
- scopus:64749109757
- ISSN
- 0099-2240
- DOI
- 10.1128/AEM.01325-08
- language
- English
- LU publication?
- yes
- id
- 0e4656d0-ee6d-4530-be21-b61a51e388cd (old id 1400783)
- date added to LUP
- 2016-04-01 11:59:46
- date last changed
- 2022-03-20 21:55:37
@article{0e4656d0-ee6d-4530-be21-b61a51e388cd,
abstract = {{The aim of this study was to clarify the pathway of electron transfer between the inner membrane components and the periplasmic chlorate reductase. Several soluble c-type cytochromes were found in the periplasm. The optical difference spectrum of dithionite-reduced periplasmic extract shows that at least one of these components is capable of acting as an electron donor to the enzyme chlorate reductase. The cytochromes were partially separated, and the fractions were analyzed by UV/visible spectroscopy to determine the ability of donating electrons to chlorate reductase. Our results show that one of the c cytochromes (6 kDa) is able to donate electrons, both to chlorate reductase and to the membrane-bound cytochrome c oxidase, whereas the roles of the remaining c cytochromes still remain to be elucidated. Peptide extracts of the c cytochromes were obtained by tryptic in-gel digestion for matrix-assisted laser desorption ionization -time of flight mass spectrometry analysis. Peptide sequences obtained indicate that the 6-kDa cytochrome c protein is similar to c cytochromes from the chlorate-reducing bacterium Dechloromonas aromatica.}},
author = {{Backlund, Anna Smedja and Bohlin, Jan and Gustavsson, Niklas and Nilsson, Thomas}},
issn = {{0099-2240}},
language = {{eng}},
number = {{8}},
pages = {{2439--2445}},
publisher = {{American Society for Microbiology}},
series = {{Applied and Environmental Microbiology}},
title = {{Periplasmic c Cytochromes and Chlorate Reduction in Ideonella dechloratans}},
url = {{http://dx.doi.org/10.1128/AEM.01325-08}},
doi = {{10.1128/AEM.01325-08}},
volume = {{75}},
year = {{2009}},
}