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Effect of dietary alpha-ketoglutarate on blood lipid profile during hypercholesterolaemia in rats

Radzki, Radoslaw P.; Bienko, Marek and Pierzynowski, Stefan LU (2009) In Scandinavian Journal of Clinical & Laboratory Investigation 69(2). p.175-180
Abstract
Objective. The aim of the study was to determine the effect of -ketoglutarate on the blood lipid profile using a rat animal model with experimentally induced hypercholesterolaemia. Material and methods. The female and male (30/30) Wistar rats had ad libitum access to a diet containing cholesterol (1 %) and lard (10 %) throughout the entire experimental period (120 days). On day 60 of the study, both the females and the males were divided into three groups, the first receiving a mixture of drinking water adjusted to pH 4.6 using HCl (control), the other two (experimental groups) receiving a solution containing 0.01 M and 0.1 M -ketoglutarate (AKG) (pH adjusted to 4.6). Blood samples were taken on days 0, 30, 60 and 120. Results. The... (More)
Objective. The aim of the study was to determine the effect of -ketoglutarate on the blood lipid profile using a rat animal model with experimentally induced hypercholesterolaemia. Material and methods. The female and male (30/30) Wistar rats had ad libitum access to a diet containing cholesterol (1 %) and lard (10 %) throughout the entire experimental period (120 days). On day 60 of the study, both the females and the males were divided into three groups, the first receiving a mixture of drinking water adjusted to pH 4.6 using HCl (control), the other two (experimental groups) receiving a solution containing 0.01 M and 0.1 M -ketoglutarate (AKG) (pH adjusted to 4.6). Blood samples were taken on days 0, 30, 60 and 120. Results. The concentrations of total cholesterol, triglycerides, HDL and LDL, respectively, in the blood serum were estimated spectrophotometrically. During the entire experimental period the total cholesterol, triglycerides and LDL levels of the control rats increased, whereas that of HDL decreased. The serum concentrations of total cholesterol, LDL and triglycerides in both the experimental groups receiving AKG decreased (days 60 to 120) (p0.05), while the HDL concentration tended to increase. The body gain in all groups receiving AKG was significantly lower than in the control group. Conclusions. These observations clearly prove that oral treatment with AKG can decrease the risk of hypercholesterolaemia developing and can lower the body weight. The relative concentrations of the plasma LDL and HDL changed to a more favourable ratio promoting good health. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
rats, nutrition, hypercholesterolaemia, Alpha-ketoglutarate, blood lipids
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
69
issue
2
pages
175 - 180
publisher
Informa Healthcare
external identifiers
  • wos:000264649400003
  • scopus:65649107718
ISSN
1502-7686
DOI
10.1080/00365510802464633
language
English
LU publication?
yes
id
ba5fd4f7-16f8-447e-bef6-3d18a368abc4 (old id 1400836)
date added to LUP
2009-06-15 12:58:49
date last changed
2017-11-12 03:48:47
@article{ba5fd4f7-16f8-447e-bef6-3d18a368abc4,
  abstract     = {Objective. The aim of the study was to determine the effect of -ketoglutarate on the blood lipid profile using a rat animal model with experimentally induced hypercholesterolaemia. Material and methods. The female and male (30/30) Wistar rats had ad libitum access to a diet containing cholesterol (1 %) and lard (10 %) throughout the entire experimental period (120 days). On day 60 of the study, both the females and the males were divided into three groups, the first receiving a mixture of drinking water adjusted to pH 4.6 using HCl (control), the other two (experimental groups) receiving a solution containing 0.01 M and 0.1 M -ketoglutarate (AKG) (pH adjusted to 4.6). Blood samples were taken on days 0, 30, 60 and 120. Results. The concentrations of total cholesterol, triglycerides, HDL and LDL, respectively, in the blood serum were estimated spectrophotometrically. During the entire experimental period the total cholesterol, triglycerides and LDL levels of the control rats increased, whereas that of HDL decreased. The serum concentrations of total cholesterol, LDL and triglycerides in both the experimental groups receiving AKG decreased (days 60 to 120) (p0.05), while the HDL concentration tended to increase. The body gain in all groups receiving AKG was significantly lower than in the control group. Conclusions. These observations clearly prove that oral treatment with AKG can decrease the risk of hypercholesterolaemia developing and can lower the body weight. The relative concentrations of the plasma LDL and HDL changed to a more favourable ratio promoting good health.},
  author       = {Radzki, Radoslaw P. and Bienko, Marek and Pierzynowski, Stefan},
  issn         = {1502-7686},
  keyword      = {rats,nutrition,hypercholesterolaemia,Alpha-ketoglutarate,blood lipids},
  language     = {eng},
  number       = {2},
  pages        = {175--180},
  publisher    = {Informa Healthcare},
  series       = {Scandinavian Journal of Clinical & Laboratory Investigation},
  title        = {Effect of dietary alpha-ketoglutarate on blood lipid profile during hypercholesterolaemia in rats},
  url          = {http://dx.doi.org/10.1080/00365510802464633},
  volume       = {69},
  year         = {2009},
}