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Gene expressions of small leucine-rich repeat proteoglycans and fibulin-5 are decreased in pelvic organ prolapse

Soderberg, Marie Westergren; Bystrom, Birgitta; Kalamajski, Sebastian LU ; Malmström, Anders LU and Ekman-Ordeberg, Gunvor (2009) In Molecular Human Reproduction 15(4). p.251-257
Abstract
Few studies are performed on the sustainability of the pelvic floor extracellular matrix important for preventing development of pelvic organ prolapse (POP). Collagens I and III, the elastin-associated proteins fibrillin-1 and fibulin-5 and the small leucine-rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin are involved in giving the tissue its mechanical properties. Para-urethral biopsies were obtained from 15 women, 6 pre- and 9 post-menopausal, with POP. Real-time reverse transcription-polymerase chain reaction and immunohistochemistry for collagen I, collagen III, fibrillin-1, fibulin-5, decorin, lumican and fibromodulin were performed and compared with 14 controls, 8 pre- and 6 post-menopausal. Statistical... (More)
Few studies are performed on the sustainability of the pelvic floor extracellular matrix important for preventing development of pelvic organ prolapse (POP). Collagens I and III, the elastin-associated proteins fibrillin-1 and fibulin-5 and the small leucine-rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin are involved in giving the tissue its mechanical properties. Para-urethral biopsies were obtained from 15 women, 6 pre- and 9 post-menopausal, with POP. Real-time reverse transcription-polymerase chain reaction and immunohistochemistry for collagen I, collagen III, fibrillin-1, fibulin-5, decorin, lumican and fibromodulin were performed and compared with 14 controls, 8 pre- and 6 post-menopausal. Statistical comparisons controlled for age changes in gene expressions. A 16-fold decrease in decorin mRNA expression, P = 0.0001, and 8-fold in lumican mRNA expression, P = 0.001, were discovered in premenopausal POP compared with matched controls. In all women with POP, there were lower gene expressions of fibromodulin, P = 0.004, and fibulin-5, P = 0.001, compared with all controls. All proteins were detectable by immunohistochemistry, showing a weaker staining for decorin in premenopausal POP. For the first time, we show substantially decreased gene signal for production of SLRPs, regulators of collagen fiber assembly and impairment in elastic fiber assembly by down-regulation of fibulin-5 in POP. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
collagen, elastin, prolapse, proteoglycan, extracellular matrix
in
Molecular Human Reproduction
volume
15
issue
4
pages
251 - 257
publisher
Oxford University Press
external identifiers
  • wos:000264394600006
  • scopus:63149176556
ISSN
1460-2407
DOI
10.1093/molehr/gap011
language
English
LU publication?
yes
id
f49a884d-bdda-4c12-a150-88a54fe822ff (old id 1401447)
date added to LUP
2009-06-15 11:13:56
date last changed
2017-08-20 03:37:16
@article{f49a884d-bdda-4c12-a150-88a54fe822ff,
  abstract     = {Few studies are performed on the sustainability of the pelvic floor extracellular matrix important for preventing development of pelvic organ prolapse (POP). Collagens I and III, the elastin-associated proteins fibrillin-1 and fibulin-5 and the small leucine-rich repeat proteoglycans (SLRPs) decorin, lumican and fibromodulin are involved in giving the tissue its mechanical properties. Para-urethral biopsies were obtained from 15 women, 6 pre- and 9 post-menopausal, with POP. Real-time reverse transcription-polymerase chain reaction and immunohistochemistry for collagen I, collagen III, fibrillin-1, fibulin-5, decorin, lumican and fibromodulin were performed and compared with 14 controls, 8 pre- and 6 post-menopausal. Statistical comparisons controlled for age changes in gene expressions. A 16-fold decrease in decorin mRNA expression, P = 0.0001, and 8-fold in lumican mRNA expression, P = 0.001, were discovered in premenopausal POP compared with matched controls. In all women with POP, there were lower gene expressions of fibromodulin, P = 0.004, and fibulin-5, P = 0.001, compared with all controls. All proteins were detectable by immunohistochemistry, showing a weaker staining for decorin in premenopausal POP. For the first time, we show substantially decreased gene signal for production of SLRPs, regulators of collagen fiber assembly and impairment in elastic fiber assembly by down-regulation of fibulin-5 in POP.},
  author       = {Soderberg, Marie Westergren and Bystrom, Birgitta and Kalamajski, Sebastian and Malmström, Anders and Ekman-Ordeberg, Gunvor},
  issn         = {1460-2407},
  keyword      = {collagen,elastin,prolapse,proteoglycan,extracellular matrix},
  language     = {eng},
  number       = {4},
  pages        = {251--257},
  publisher    = {Oxford University Press},
  series       = {Molecular Human Reproduction},
  title        = {Gene expressions of small leucine-rich repeat proteoglycans and fibulin-5 are decreased in pelvic organ prolapse},
  url          = {http://dx.doi.org/10.1093/molehr/gap011},
  volume       = {15},
  year         = {2009},
}