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Post-genomic insights into the plant polysaccharide degradation potential of Aspergillus nidulans and comparison to Aspergillus niger and Aspergillus oryzae

Coutinho, Pedro M.; Andersen, Mikael R.; Kolenová, Katarina LU ; vanKuyk, Patricia A.; Benoit, Isabelle; Gruben, Birgit S.; Trejo-Aguilar, Blanca; Visser, Hans; van Solingen, Piet and Pakula, Tiina, et al. (2009) In Fungal Genetics and Biology 46(Suppl 1). p.161-169
Abstract
The plant polysaccharide degradative potential of Aspergillus nidulans was analysed in detail and compared to that of Aspergillus niger and Aspergillus oryzae using a combination of bioinformatics, physiology and transcriptomics. Manual verification indicated that 28.4% of the A. nidulans ORFs analysed in this study do not contain a secretion signal, of which 40% may be secreted through a non-classical method. While significant differences were found between the species in the numbers of ORFs assigned to the relevant CAZy families, no significant difference was observed in growth on polysaccharides. Growth differences were observed between the Aspergilli and Podospora anserina, which has a more different genomic potential for... (More)
The plant polysaccharide degradative potential of Aspergillus nidulans was analysed in detail and compared to that of Aspergillus niger and Aspergillus oryzae using a combination of bioinformatics, physiology and transcriptomics. Manual verification indicated that 28.4% of the A. nidulans ORFs analysed in this study do not contain a secretion signal, of which 40% may be secreted through a non-classical method. While significant differences were found between the species in the numbers of ORFs assigned to the relevant CAZy families, no significant difference was observed in growth on polysaccharides. Growth differences were observed between the Aspergilli and Podospora anserina, which has a more different genomic potential for polysaccharide degradation, suggesting that large genomic differences are required to cause growth differences oil polysaccharides, Differences were also detected between the Aspergilli in the presence Of putative regulatory sequences in the promoters of the ORFs Of this Study and correlation of the presence Of putative XlnR binding sites to induction by xylose was detected for A. niger. These data demonstrate differences at genome content, Substrate specificity of the enzymes and gene regulation in these three Aspergilli, which likely reflect their individual adaptation to their natural biotope. (C) 2008 Elsevier Inc. All rights reserved. (Less)
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keywords
Micro array analysis, Promoter analysis, CAZy, Aspergillus, Plant polysaccharide degradation, XlnR, Substrate specificity
in
Fungal Genetics and Biology
volume
46
issue
Suppl 1
pages
161 - 169
publisher
Elsevier
external identifiers
  • wos:000264227100015
  • scopus:67650578182
ISSN
1087-1845
DOI
10.1016/j.fgb.2008.07.020
language
English
LU publication?
yes
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4e157806-e8ab-43cb-a808-db37361c1c3a (old id 1404892)
date added to LUP
2009-06-10 15:58:03
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2017-12-10 04:15:53
@article{4e157806-e8ab-43cb-a808-db37361c1c3a,
  abstract     = {The plant polysaccharide degradative potential of Aspergillus nidulans was analysed in detail and compared to that of Aspergillus niger and Aspergillus oryzae using a combination of bioinformatics, physiology and transcriptomics. Manual verification indicated that 28.4% of the A. nidulans ORFs analysed in this study do not contain a secretion signal, of which 40% may be secreted through a non-classical method. While significant differences were found between the species in the numbers of ORFs assigned to the relevant CAZy families, no significant difference was observed in growth on polysaccharides. Growth differences were observed between the Aspergilli and Podospora anserina, which has a more different genomic potential for polysaccharide degradation, suggesting that large genomic differences are required to cause growth differences oil polysaccharides, Differences were also detected between the Aspergilli in the presence Of putative regulatory sequences in the promoters of the ORFs Of this Study and correlation of the presence Of putative XlnR binding sites to induction by xylose was detected for A. niger. These data demonstrate differences at genome content, Substrate specificity of the enzymes and gene regulation in these three Aspergilli, which likely reflect their individual adaptation to their natural biotope. (C) 2008 Elsevier Inc. All rights reserved.},
  author       = {Coutinho, Pedro M. and Andersen, Mikael R. and Kolenová, Katarina and vanKuyk, Patricia A. and Benoit, Isabelle and Gruben, Birgit S. and Trejo-Aguilar, Blanca and Visser, Hans and van Solingen, Piet and Pakula, Tiina and Seiboth, Bernard and Battaglia, Evy and Aguilar-Osorio, Guillermo and de Jong, Jan F. and Ohm, Robin A. and Aguilar, Mariana and Henrissat, Bernard and Nielsen, Jens and Stålbrand, Henrik and de Vries, Ronald P.},
  issn         = {1087-1845},
  keyword      = {Micro array analysis,Promoter analysis,CAZy,Aspergillus,Plant polysaccharide degradation,XlnR,Substrate specificity},
  language     = {eng},
  number       = {Suppl 1},
  pages        = {161--169},
  publisher    = {Elsevier},
  series       = {Fungal Genetics and Biology},
  title        = {Post-genomic insights into the plant polysaccharide degradation potential of Aspergillus nidulans and comparison to Aspergillus niger and Aspergillus oryzae},
  url          = {http://dx.doi.org/10.1016/j.fgb.2008.07.020},
  volume       = {46},
  year         = {2009},
}