Impact of DNA polymerases and their buffer systems on quantitative real-time PCR
(2004) In Journal of Clinical Microbiology 42(1). p.408-411- Abstract
- An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/140516
- author
- Wolffs, Petra LU ; Grage, Halfdan LU ; Hagberg, Oskar LU and Rådström, Peter LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Clinical Microbiology
- volume
- 42
- issue
- 1
- pages
- 408 - 411
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000188121800070
- pmid:14715792
- scopus:0347723809
- ISSN
- 1098-660X
- DOI
- 10.1128/JCM.42.1.408-411.2004
- language
- English
- LU publication?
- yes
- id
- 9f6a90da-b724-4478-8343-bd81abacc34b (old id 140516)
- date added to LUP
- 2016-04-01 16:12:08
- date last changed
- 2022-03-22 17:06:02
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