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Impact of DNA polymerases and their buffer systems on quantitative real-time PCR

Wolffs, Petra LU ; Grage, Halfdan LU ; Hagberg, Oskar LU and Rådström, Peter LU (2004) In Journal of Clinical Microbiology 42(1). p.408-411
Abstract
An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Microbiology
volume
42
issue
1
pages
408 - 411
publisher
American Society for Microbiology
external identifiers
  • wos:000188121800070
  • pmid:14715792
  • scopus:0347723809
ISSN
1098-660X
DOI
10.1128/JCM.42.1.408-411.2004
language
English
LU publication?
yes
id
9f6a90da-b724-4478-8343-bd81abacc34b (old id 140516)
date added to LUP
2007-06-28 14:45:12
date last changed
2017-09-10 04:31:20
@article{9f6a90da-b724-4478-8343-bd81abacc34b,
  abstract     = {An investigation of the influence of five DNA polymerase-buffer systems on real-time PCR showed that the choice of both DNA polymerase and the buffer system affected the amplification efficiency as well as the detection window. The analytical repeatability of the data for different systems changed clearly, leading us to conclude that basing quantitative measurements on single-data-set standard curves can lead to significant errors.},
  author       = {Wolffs, Petra and Grage, Halfdan and Hagberg, Oskar and Rådström, Peter},
  issn         = {1098-660X},
  language     = {eng},
  number       = {1},
  pages        = {408--411},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Clinical Microbiology},
  title        = {Impact of DNA polymerases and their buffer systems on quantitative real-time PCR},
  url          = {http://dx.doi.org/10.1128/JCM.42.1.408-411.2004},
  volume       = {42},
  year         = {2004},
}