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Agarose-coated anion exchanger prevents cell-adsorbent interactions

Viloria-Cols, Maria LU ; Hatti-Kaul, Rajni LU and Mattiasson, Bo LU (2004) In Journal of Chromatography A 1043(2). p.195-200
Abstract
A common problem during recovery of bioproducts by adsorption from particulate broths is fouling of the adsorbent material as a result of the interaction of cells and cell debris, which present negative charges, with the positively charged anion exchangers commonly used in bioprocesses. The effect of shielding an adsorbent with a layer of agarose on reducing the binding of cells while still allowing the low-molecular-mass bioproducts to be adsorbed was studied. Coating the anion-exchange resin Amberlite IRA-400 with agarose followed by cross-linking the agarose layer effectively prevented the binding of Escherichia coli, Saccharomyces cerevisiae, and Lactobacillus casei cells but allowed binding of lactic acid to the adsorbent. The... (More)
A common problem during recovery of bioproducts by adsorption from particulate broths is fouling of the adsorbent material as a result of the interaction of cells and cell debris, which present negative charges, with the positively charged anion exchangers commonly used in bioprocesses. The effect of shielding an adsorbent with a layer of agarose on reducing the binding of cells while still allowing the low-molecular-mass bioproducts to be adsorbed was studied. Coating the anion-exchange resin Amberlite IRA-400 with agarose followed by cross-linking the agarose layer effectively prevented the binding of Escherichia coli, Saccharomyces cerevisiae, and Lactobacillus casei cells but allowed binding of lactic acid to the adsorbent. The cross-linked agarose layer was stable during recycling of the adsorbent. (C) 2004 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Fluidized bed adsorption, Fouling, Agarose coating, Amberlite IRA-400, Cells, Adsorbents, Lactic acid
in
Journal of Chromatography A
volume
1043
issue
2
pages
195 - 200
publisher
Elsevier
external identifiers
  • wos:000222807500008
  • pmid:15330092
  • scopus:3042839749
ISSN
0021-9673
DOI
10.1016/j.chroma.2004.05.086
language
English
LU publication?
yes
id
05fd99aa-69a1-466a-90f8-7fa1129671ce (old id 140627)
date added to LUP
2007-07-03 09:29:17
date last changed
2017-01-01 06:46:32
@article{05fd99aa-69a1-466a-90f8-7fa1129671ce,
  abstract     = {A common problem during recovery of bioproducts by adsorption from particulate broths is fouling of the adsorbent material as a result of the interaction of cells and cell debris, which present negative charges, with the positively charged anion exchangers commonly used in bioprocesses. The effect of shielding an adsorbent with a layer of agarose on reducing the binding of cells while still allowing the low-molecular-mass bioproducts to be adsorbed was studied. Coating the anion-exchange resin Amberlite IRA-400 with agarose followed by cross-linking the agarose layer effectively prevented the binding of Escherichia coli, Saccharomyces cerevisiae, and Lactobacillus casei cells but allowed binding of lactic acid to the adsorbent. The cross-linked agarose layer was stable during recycling of the adsorbent. (C) 2004 Elsevier B.V. All rights reserved.},
  author       = {Viloria-Cols, Maria and Hatti-Kaul, Rajni and Mattiasson, Bo},
  issn         = {0021-9673},
  keyword      = {Fluidized bed adsorption,Fouling,Agarose coating,Amberlite IRA-400,Cells,Adsorbents,Lactic acid},
  language     = {eng},
  number       = {2},
  pages        = {195--200},
  publisher    = {Elsevier},
  series       = {Journal of Chromatography A},
  title        = {Agarose-coated anion exchanger prevents cell-adsorbent interactions},
  url          = {http://dx.doi.org/10.1016/j.chroma.2004.05.086},
  volume       = {1043},
  year         = {2004},
}