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Starch hydrolysing Bacillus halodurans isolates from a Kenyan soda lake

Hashim, Suhaila LU ; Delgado, Osvaldo LU ; Hatti-Kaul, Rajni LU ; Mulaa, Francis J and Mattiasson, Bo LU (2004) In Biotechnology Letters 26(10). p.823-828
Abstract
Fourteen obligate alkaliphilic and halotolerant bacterial isolates, exhibiting extracellular amylase activity at 55degreesC and pH 10, were isolated from hot springs around Lake Bogoria, Kenya. From 16S rDNA sequence analysis, nine isolates shared 100% identity with Bacillus halodurans strain DSM 497(T), while the rest shared 99% identity with alkaliphilic Bacillus species A-59. PCR of the intergenic spacer region between 16S and 23S rRNA genes (ISR-PCR) divided the isolates into two groups, while tDNA-PCR divided them into three groups. Bacillus halodurans DSM 497T had a different ISR pattern from the isolates, while it had a tDNA-PCR profile similar to the group that shared 99% identity with alkaliphilic Bacillus species A-59. All... (More)
Fourteen obligate alkaliphilic and halotolerant bacterial isolates, exhibiting extracellular amylase activity at 55degreesC and pH 10, were isolated from hot springs around Lake Bogoria, Kenya. From 16S rDNA sequence analysis, nine isolates shared 100% identity with Bacillus halodurans strain DSM 497(T), while the rest shared 99% identity with alkaliphilic Bacillus species A-59. PCR of the intergenic spacer region between 16S and 23S rRNA genes (ISR-PCR) divided the isolates into two groups, while tDNA-PCR divided them into three groups. Bacillus halodurans DSM 497T had a different ISR pattern from the isolates, while it had a tDNA-PCR profile similar to the group that shared 99% identity with alkaliphilic Bacillus species A-59. All isolates hydrolysed soluble starch as well as amylose, amylopectin and pullulan. The amylase activity (1.2 - 1.8 U ml(-1)) in the culture broths had an optimum temperature of 55 - 65degreesC, was stimulated by 1 mM Ca2+, and was either partially (16 - 30%) or completely inhibited by 1 mM EDTA. Activity staining of the cell-free culture supernatant from the isolates revealed five alkaline active amylase bands. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Letters
volume
26
issue
10
pages
823 - 828
publisher
Springer
external identifiers
  • pmid:15269555
  • wos:000221101800010
  • scopus:3342881954
ISSN
1573-6776
DOI
10.1023/B:BILE.0000025885.19910.d7
language
English
LU publication?
yes
id
cc7796ee-ca6a-4674-805c-1172baf68faf (old id 140701)
date added to LUP
2007-07-02 11:23:01
date last changed
2017-05-21 04:25:43
@article{cc7796ee-ca6a-4674-805c-1172baf68faf,
  abstract     = {Fourteen obligate alkaliphilic and halotolerant bacterial isolates, exhibiting extracellular amylase activity at 55degreesC and pH 10, were isolated from hot springs around Lake Bogoria, Kenya. From 16S rDNA sequence analysis, nine isolates shared 100% identity with Bacillus halodurans strain DSM 497(T), while the rest shared 99% identity with alkaliphilic Bacillus species A-59. PCR of the intergenic spacer region between 16S and 23S rRNA genes (ISR-PCR) divided the isolates into two groups, while tDNA-PCR divided them into three groups. Bacillus halodurans DSM 497T had a different ISR pattern from the isolates, while it had a tDNA-PCR profile similar to the group that shared 99% identity with alkaliphilic Bacillus species A-59. All isolates hydrolysed soluble starch as well as amylose, amylopectin and pullulan. The amylase activity (1.2 - 1.8 U ml(-1)) in the culture broths had an optimum temperature of 55 - 65degreesC, was stimulated by 1 mM Ca2+, and was either partially (16 - 30%) or completely inhibited by 1 mM EDTA. Activity staining of the cell-free culture supernatant from the isolates revealed five alkaline active amylase bands.},
  author       = {Hashim, Suhaila and Delgado, Osvaldo and Hatti-Kaul, Rajni and Mulaa, Francis J and Mattiasson, Bo},
  issn         = {1573-6776},
  language     = {eng},
  number       = {10},
  pages        = {823--828},
  publisher    = {Springer},
  series       = {Biotechnology Letters},
  title        = {Starch hydrolysing Bacillus halodurans isolates from a Kenyan soda lake},
  url          = {http://dx.doi.org/10.1023/B:BILE.0000025885.19910.d7},
  volume       = {26},
  year         = {2004},
}