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Hydrolysis of the cubic liquid-crystalline phase of glyceryl monooleate by human pancreatic lipases

Zhou, Li LU ; Landh, T; Sternby, Berit LU and Nilsson, Åke LU (2002) Conference on Lipid and Polymer-Lipid Systems, 2002 In Lipid and Polymer-Lipid Systems (Progress in Colloid and Polymer Science) 120. p.92-98
Abstract
Glyceryl monooleate (GMO) and other unsaturated long-chain monoglycerides show peculiar phase behaviour characterised by the presence of one or more cubic liquid-crystalline phases. In the present study, hydrolysis of cubic lipid-water phases formed by GMO were determined. Human pancreatic carboxyl ester lipase (CEL), colipase-dependent lipase with colipase, human duodenal contents and rat pancreas homogenate were added and the release of free oleic acid from GMO was determined. It was found that pancreatic enzymes hydrolyse GMO cubic lipid-water phases in vitro. In the presence of concentrations of sodium taurocholate, sodium taourodeoxycholate or sodium glycocholate, above 3 mM, the hydrolysis by pancreatic enzymes was stimulated. The... (More)
Glyceryl monooleate (GMO) and other unsaturated long-chain monoglycerides show peculiar phase behaviour characterised by the presence of one or more cubic liquid-crystalline phases. In the present study, hydrolysis of cubic lipid-water phases formed by GMO were determined. Human pancreatic carboxyl ester lipase (CEL), colipase-dependent lipase with colipase, human duodenal contents and rat pancreas homogenate were added and the release of free oleic acid from GMO was determined. It was found that pancreatic enzymes hydrolyse GMO cubic lipid-water phases in vitro. In the presence of concentrations of sodium taurocholate, sodium taourodeoxycholate or sodium glycocholate, above 3 mM, the hydrolysis by pancreatic enzymes was stimulated. The optimal pH for the hydrolysis of GMO by CEL was 7.5-8.5, by colipase-dependent lipase 6-9 with a peak at 6.5 and by rat pancreas homogenate 6-8.5 with a peak at 7.3. The hydrolysis of GMO with human duodenal content was slow but increased with time. These results indicated that pancreatic enzymes hydrolyse GMO in cubic lipid-water phases. The hydrolysis is effected by pH and the concentration of bile salts. (Less)
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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
bile, hydrolysis, glyceryl monooleate, cubic liquid-crystalline phase, lipases, salt
in
Lipid and Polymer-Lipid Systems (Progress in Colloid and Polymer Science)
volume
120
pages
92 - 98
publisher
Springer
conference name
Conference on Lipid and Polymer-Lipid Systems, 2002
external identifiers
  • wos:000177564900012
  • scopus:0036954679
ISSN
0340-255X
ISBN
978-3-540-43001-8
DOI
10.1007/s02882013
language
English
LU publication?
yes
id
9b4a3655-11e7-40ab-a910-a2a7740c139a (old id 1407254)
date added to LUP
2009-06-02 07:58:13
date last changed
2017-01-01 06:36:58
@inproceedings{9b4a3655-11e7-40ab-a910-a2a7740c139a,
  abstract     = {Glyceryl monooleate (GMO) and other unsaturated long-chain monoglycerides show peculiar phase behaviour characterised by the presence of one or more cubic liquid-crystalline phases. In the present study, hydrolysis of cubic lipid-water phases formed by GMO were determined. Human pancreatic carboxyl ester lipase (CEL), colipase-dependent lipase with colipase, human duodenal contents and rat pancreas homogenate were added and the release of free oleic acid from GMO was determined. It was found that pancreatic enzymes hydrolyse GMO cubic lipid-water phases in vitro. In the presence of concentrations of sodium taurocholate, sodium taourodeoxycholate or sodium glycocholate, above 3 mM, the hydrolysis by pancreatic enzymes was stimulated. The optimal pH for the hydrolysis of GMO by CEL was 7.5-8.5, by colipase-dependent lipase 6-9 with a peak at 6.5 and by rat pancreas homogenate 6-8.5 with a peak at 7.3. The hydrolysis of GMO with human duodenal content was slow but increased with time. These results indicated that pancreatic enzymes hydrolyse GMO in cubic lipid-water phases. The hydrolysis is effected by pH and the concentration of bile salts.},
  author       = {Zhou, Li and Landh, T and Sternby, Berit and Nilsson, Åke},
  booktitle    = {Lipid and Polymer-Lipid Systems (Progress in Colloid and Polymer Science)},
  isbn         = {978-3-540-43001-8},
  issn         = {0340-255X},
  keyword      = {bile,hydrolysis,glyceryl monooleate,cubic liquid-crystalline phase,lipases,salt},
  language     = {eng},
  pages        = {92--98},
  publisher    = {Springer},
  title        = {Hydrolysis of the cubic liquid-crystalline phase of glyceryl monooleate by human pancreatic lipases},
  url          = {http://dx.doi.org/10.1007/s02882013},
  volume       = {120},
  year         = {2002},
}