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Lipase-producing microorganisms from a Kenyan alkaline soda lake

Vargas Calle, Virginia LU ; Delgado, Osvaldo LU ; Hatti-Kaul, Rajni LU and Mattiasson, Bo LU (2004) In Biotechnology Letters 26(2). p.81-86
Abstract
Lipolytic enzyme production of 150 isolated strains from samples of Lake Bogoria (Kenya) was examined. Among these, fifteen isolates were selected on the basis of their lipolytic activities and subjected to morphological and 16S rRNA gene sequencing analyses for their identification. All the microorganisms have been selected under culture conditions with pH ranges between 7-10 and temperatures of 37-55degreesC. Most of them showed optimal growth at 37degreesC and tolerated salinity up to 10% (w/v). Ten of the isolates were Gram-negative, nine of which were closely related to the Pseudomonas cluster and one to the Halomonas cluster sharing high similarity profile with Halomonas desiderata. The remaining Gram-positive isolates were closely... (More)
Lipolytic enzyme production of 150 isolated strains from samples of Lake Bogoria (Kenya) was examined. Among these, fifteen isolates were selected on the basis of their lipolytic activities and subjected to morphological and 16S rRNA gene sequencing analyses for their identification. All the microorganisms have been selected under culture conditions with pH ranges between 7-10 and temperatures of 37-55degreesC. Most of them showed optimal growth at 37degreesC and tolerated salinity up to 10% (w/v). Ten of the isolates were Gram-negative, nine of which were closely related to the Pseudomonas cluster and one to the Halomonas cluster sharing high similarity profile with Halomonas desiderata. The remaining Gram-positive isolates were closely related to the Bacillus cluster, and were grouped with Bacillus halodurans, Bacillus alcalophilus and Bacillus licheniformis. Four members of the Bacillus cluster and the Halomonas sp. produced lipolytic activity under alkaline conditions, while others did so at neutral pH values. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Letters
volume
26
issue
2
pages
81 - 86
publisher
Springer
external identifiers
  • wos:000188285000001
  • pmid:15000472
  • scopus:1042303082
ISSN
1573-6776
DOI
10.1023/B:BILE.0000012898.50608.12
language
English
LU publication?
yes
id
2c84af0b-0359-4ccf-8acd-0638f824306a (old id 140759)
date added to LUP
2007-07-03 09:25:37
date last changed
2017-12-10 04:29:52
@article{2c84af0b-0359-4ccf-8acd-0638f824306a,
  abstract     = {Lipolytic enzyme production of 150 isolated strains from samples of Lake Bogoria (Kenya) was examined. Among these, fifteen isolates were selected on the basis of their lipolytic activities and subjected to morphological and 16S rRNA gene sequencing analyses for their identification. All the microorganisms have been selected under culture conditions with pH ranges between 7-10 and temperatures of 37-55degreesC. Most of them showed optimal growth at 37degreesC and tolerated salinity up to 10% (w/v). Ten of the isolates were Gram-negative, nine of which were closely related to the Pseudomonas cluster and one to the Halomonas cluster sharing high similarity profile with Halomonas desiderata. The remaining Gram-positive isolates were closely related to the Bacillus cluster, and were grouped with Bacillus halodurans, Bacillus alcalophilus and Bacillus licheniformis. Four members of the Bacillus cluster and the Halomonas sp. produced lipolytic activity under alkaline conditions, while others did so at neutral pH values.},
  author       = {Vargas Calle, Virginia and Delgado, Osvaldo and Hatti-Kaul, Rajni and Mattiasson, Bo},
  issn         = {1573-6776},
  language     = {eng},
  number       = {2},
  pages        = {81--86},
  publisher    = {Springer},
  series       = {Biotechnology Letters},
  title        = {Lipase-producing microorganisms from a Kenyan alkaline soda lake},
  url          = {http://dx.doi.org/10.1023/B:BILE.0000012898.50608.12},
  volume       = {26},
  year         = {2004},
}