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Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes

Jobe, Njainday Pulo LU ; Živicová, Veronika; Mifková, Alžběta; Rösel, Daniel; Dvořánková, Barbora; Kodet, Ondřej; Strnad, Hynek; Kolář, Michal; Šedo, Aleksi and Smetana, Karel, et al. (2018) In Histochemistry and Cell Biology p.1-14
Abstract

Melanoma represents a malignant disease with steadily increasing incidence. UV-irradiation is a recognized key factor in melanoma initiation. Therefore, the efficient prevention of UV tissue damage bears a critical potential for melanoma prevention. In this study, we tested the effect of UV irradiation of normal keratinocytes and their consequent interaction with normal and cancer-associated fibroblasts isolated from melanoma, respectively. Using this model of UV influenced microenvironment, we measured melanoma cell migration in 3-D collagen gels. These interactions were studied using DNA microarray technology, immunofluorescence staining, single cell electrophoresis assay, viability (dead/life) cell detection methods, and migration... (More)

Melanoma represents a malignant disease with steadily increasing incidence. UV-irradiation is a recognized key factor in melanoma initiation. Therefore, the efficient prevention of UV tissue damage bears a critical potential for melanoma prevention. In this study, we tested the effect of UV irradiation of normal keratinocytes and their consequent interaction with normal and cancer-associated fibroblasts isolated from melanoma, respectively. Using this model of UV influenced microenvironment, we measured melanoma cell migration in 3-D collagen gels. These interactions were studied using DNA microarray technology, immunofluorescence staining, single cell electrophoresis assay, viability (dead/life) cell detection methods, and migration analysis. We observed that three 10 mJ/cm2 fractions at equal intervals over 72 h applied on keratinocytes lead to a 50% increase (p < 0.05) in in vitro invasion of melanoma cells. The introduction cancer-associated fibroblasts to such model further significantly stimulated melanoma cells in vitro invasiveness to a higher extent than normal fibroblasts. A panel of candidate gene products responsible for facilitation of melanoma cells invasion was defined with emphasis on IL-6, IL-8, and CXCL-1. In conclusion, this study demonstrates a synergistic effect between cancer microenvironment and UV irradiation in melanoma invasiveness under in vitro condition.

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publication status
in press
subject
keywords
Cancer microenvironment, Cancer-associated fibroblasts, Chemokine, Cytokine, Keratinocytes, Melanoma
in
Histochemistry and Cell Biology
pages
14 pages
publisher
Springer
external identifiers
  • scopus:85041895906
ISSN
0948-6143
DOI
10.1007/s00418-018-1650-4
language
English
LU publication?
yes
id
140d43d9-027f-4380-abec-c47b999ae257
date added to LUP
2018-03-01 10:30:24
date last changed
2018-06-01 03:00:25
@article{140d43d9-027f-4380-abec-c47b999ae257,
  abstract     = {<p>Melanoma represents a malignant disease with steadily increasing incidence. UV-irradiation is a recognized key factor in melanoma initiation. Therefore, the efficient prevention of UV tissue damage bears a critical potential for melanoma prevention. In this study, we tested the effect of UV irradiation of normal keratinocytes and their consequent interaction with normal and cancer-associated fibroblasts isolated from melanoma, respectively. Using this model of UV influenced microenvironment, we measured melanoma cell migration in 3-D collagen gels. These interactions were studied using DNA microarray technology, immunofluorescence staining, single cell electrophoresis assay, viability (dead/life) cell detection methods, and migration analysis. We observed that three 10 mJ/cm<sup>2</sup> fractions at equal intervals over 72 h applied on keratinocytes lead to a 50% increase (p &lt; 0.05) in in vitro invasion of melanoma cells. The introduction cancer-associated fibroblasts to such model further significantly stimulated melanoma cells in vitro invasiveness to a higher extent than normal fibroblasts. A panel of candidate gene products responsible for facilitation of melanoma cells invasion was defined with emphasis on IL-6, IL-8, and CXCL-1. In conclusion, this study demonstrates a synergistic effect between cancer microenvironment and UV irradiation in melanoma invasiveness under in vitro condition.</p>},
  author       = {Jobe, Njainday Pulo and Živicová, Veronika and Mifková, Alžběta and Rösel, Daniel and Dvořánková, Barbora and Kodet, Ondřej and Strnad, Hynek and Kolář, Michal and Šedo, Aleksi and Smetana, Karel and Strnadová, Karolina and Brábek, Jan and Lacina, Lukáš},
  issn         = {0948-6143},
  keyword      = {Cancer microenvironment,Cancer-associated fibroblasts,Chemokine,Cytokine,Keratinocytes,Melanoma},
  language     = {eng},
  month        = {02},
  pages        = {1--14},
  publisher    = {Springer},
  series       = {Histochemistry and Cell Biology},
  title        = {Fibroblasts potentiate melanoma cells in vitro invasiveness induced by UV-irradiated keratinocytes},
  url          = {http://dx.doi.org/10.1007/s00418-018-1650-4},
  year         = {2018},
}