Advanced

Analytical Model Study of Dendrimer/DNA Complexes.

Qamhieh, Khawla; Nylander, Tommy LU and Ainalem, Marie-Louise LU (2009) In Biomacromolecules 10. p.1720-1726
Abstract
The interaction between positively charged poly(amido amine) (PAMAM) dendrimers of generation 4 and DNA has been investigated for two DNA lengths; 2000 basepairs (bp; L = 680 nm) and 4331 bp (L = 1472.5 nm) using a theoretical model by Schiessel for a semiflexible polyelectrolyte and hard spheres. The model was modified to take into account that the dendrimers are to be regarded as soft spheres, that is, the radius is not constant when the DNA interact with the dendrimer. For the shorter and longer DNA, the estimated optimal wrapping length, l(opt) is approximately 15.69 and approximately 12.25 nm, respectively, for dendrimers that retain their original size (R(o) = 2.25 nm) upon DNA interaction. However, the values of l(opt) for the... (More)
The interaction between positively charged poly(amido amine) (PAMAM) dendrimers of generation 4 and DNA has been investigated for two DNA lengths; 2000 basepairs (bp; L = 680 nm) and 4331 bp (L = 1472.5 nm) using a theoretical model by Schiessel for a semiflexible polyelectrolyte and hard spheres. The model was modified to take into account that the dendrimers are to be regarded as soft spheres, that is, the radius is not constant when the DNA interact with the dendrimer. For the shorter and longer DNA, the estimated optimal wrapping length, l(opt) is approximately 15.69 and approximately 12.25 nm, respectively, for dendrimers that retain their original size (R(o) = 2.25 nm) upon DNA interaction. However, the values of l(opt) for the dendrimers that were considered to have a radius of (R = 0.4R(o)) 0.9 nm were 9.3 and 9.4 nm for the short and long DNA, respectively, and the effect due to the DNA length is no longer observed. For l(opt) = 10.88 nm, which is the length needed to neutralize the 64 positive charges of the G4 dendrimer, the maximum number of dendrimers per DNA (N(max)) was approximately 76 for the shorter DNA, which is larger than the corresponding experimental value of 35 for 2000 bp DNA. For the longer DNA, N(max) approximately 160, which is close to the experimental value of 140 for the 4331 bp DNA. Charge inversion of the dendrimer is only observed when they retain their size or only slightly contract upon DNA interaction. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
10
pages
1720 - 1726
publisher
The American Chemical Society
external identifiers
  • wos:000268139300007
  • pmid:19438230
  • scopus:67650473625
ISSN
1526-4602
DOI
10.1021/bm9000662
language
English
LU publication?
yes
id
65ac8fd4-e139-41d5-91c5-955b9f4c444b (old id 1412352)
date added to LUP
2009-06-11 09:37:10
date last changed
2017-09-17 04:46:00
@article{65ac8fd4-e139-41d5-91c5-955b9f4c444b,
  abstract     = {The interaction between positively charged poly(amido amine) (PAMAM) dendrimers of generation 4 and DNA has been investigated for two DNA lengths; 2000 basepairs (bp; L = 680 nm) and 4331 bp (L = 1472.5 nm) using a theoretical model by Schiessel for a semiflexible polyelectrolyte and hard spheres. The model was modified to take into account that the dendrimers are to be regarded as soft spheres, that is, the radius is not constant when the DNA interact with the dendrimer. For the shorter and longer DNA, the estimated optimal wrapping length, l(opt) is approximately 15.69 and approximately 12.25 nm, respectively, for dendrimers that retain their original size (R(o) = 2.25 nm) upon DNA interaction. However, the values of l(opt) for the dendrimers that were considered to have a radius of (R = 0.4R(o)) 0.9 nm were 9.3 and 9.4 nm for the short and long DNA, respectively, and the effect due to the DNA length is no longer observed. For l(opt) = 10.88 nm, which is the length needed to neutralize the 64 positive charges of the G4 dendrimer, the maximum number of dendrimers per DNA (N(max)) was approximately 76 for the shorter DNA, which is larger than the corresponding experimental value of 35 for 2000 bp DNA. For the longer DNA, N(max) approximately 160, which is close to the experimental value of 140 for the 4331 bp DNA. Charge inversion of the dendrimer is only observed when they retain their size or only slightly contract upon DNA interaction.},
  author       = {Qamhieh, Khawla and Nylander, Tommy and Ainalem, Marie-Louise},
  issn         = {1526-4602},
  language     = {eng},
  pages        = {1720--1726},
  publisher    = {The American Chemical Society},
  series       = {Biomacromolecules},
  title        = {Analytical Model Study of Dendrimer/DNA Complexes.},
  url          = {http://dx.doi.org/10.1021/bm9000662},
  volume       = {10},
  year         = {2009},
}