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Telomeric associations correlate with telomere length reduction and clonal chromosome aberrations in giant cell tumor of bone.

Gebre-Medhin, Samuel LU ; Broberg Palmgren, Karin LU ; Jonson, Tord LU ; Gorunova, Ludmila LU ; Vult von Steyern, Fredrik LU ; Brosjö, O; Jin, Yuesheng LU ; Gisselsson Dahlén, Margareta LU ; Panagopoulos, Ioannis LU and Mandahl, Nils LU , et al. (2009) In Cytogenetic and Genome Research2002-01-01+01:00 124(2). p.121-127
Abstract
Giant cell tumor of bone (GCTB) is characterized cytogenetically by frequent telomeric associations (tas). To explore the mechanisms behind the formation of tas in GCTB and to investigate their karyotypic consequences, the frequencies of tas and clonal aberrations other than tas in 20 GCTBs were compared to telomere length and status, as assessed by quantitative PCR, fluorescence in situ hybridization (FISH), and expression levels of four genes involved in telomere maintenance. Based on the G-banding results, the tumors were divided into two groups, one with a high frequency of tas and one with a low frequency. Clonal aberrations were found to be restricted to the group with a high level of tas, and the same group showed a significantly... (More)
Giant cell tumor of bone (GCTB) is characterized cytogenetically by frequent telomeric associations (tas). To explore the mechanisms behind the formation of tas in GCTB and to investigate their karyotypic consequences, the frequencies of tas and clonal aberrations other than tas in 20 GCTBs were compared to telomere length and status, as assessed by quantitative PCR, fluorescence in situ hybridization (FISH), and expression levels of four genes involved in telomere maintenance. Based on the G-banding results, the tumors were divided into two groups, one with a high frequency of tas and one with a low frequency. Clonal aberrations were found to be restricted to the group with a high level of tas, and the same group showed a significantly larger reduction in telomere length in tumor cells compared to peripheral blood cells. Furthermore, 65 out of 66 tas analyzed by FISH were negative for telomeric sequences. The expression levels of TERT, TERF1, TERF2, and POT1 did not correlate with telomere length or the frequency of tas. Thus, the present findings provide strong support for the notion that decreased telomere length is a prerequisite for tas in GCTBs and that the clonal changes occurring in GCTBs are derived from tas. (Less)
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keywords
Telomere-Binding Proteins: metabolism, Telomere-Binding Proteins: genetics, Telomere: metabolism, Telomerase: metabolism, Giant Cell Tumor of Bone: genetics, Telomerase: genetics, Telomeric Repeat Binding Protein 2: genetics, Telomeric Repeat Binding Protein 2: metabolism
in
Cytogenetic and Genome Research2002-01-01+01:00
volume
124
issue
2
pages
121 - 127
publisher
Karger
external identifiers
  • wos:000265863900002
  • pmid:19420923
  • scopus:65649147154
ISSN
1424-859X
DOI
10.1159/000207516
language
English
LU publication?
yes
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cd7a4e59-2304-4928-9d1e-3b55f656256a (old id 1412604)
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http://www.ncbi.nlm.nih.gov/pubmed/19420923?dopt=Abstract
date added to LUP
2009-06-01 14:21:18
date last changed
2017-08-27 05:41:43
@article{cd7a4e59-2304-4928-9d1e-3b55f656256a,
  abstract     = {Giant cell tumor of bone (GCTB) is characterized cytogenetically by frequent telomeric associations (tas). To explore the mechanisms behind the formation of tas in GCTB and to investigate their karyotypic consequences, the frequencies of tas and clonal aberrations other than tas in 20 GCTBs were compared to telomere length and status, as assessed by quantitative PCR, fluorescence in situ hybridization (FISH), and expression levels of four genes involved in telomere maintenance. Based on the G-banding results, the tumors were divided into two groups, one with a high frequency of tas and one with a low frequency. Clonal aberrations were found to be restricted to the group with a high level of tas, and the same group showed a significantly larger reduction in telomere length in tumor cells compared to peripheral blood cells. Furthermore, 65 out of 66 tas analyzed by FISH were negative for telomeric sequences. The expression levels of TERT, TERF1, TERF2, and POT1 did not correlate with telomere length or the frequency of tas. Thus, the present findings provide strong support for the notion that decreased telomere length is a prerequisite for tas in GCTBs and that the clonal changes occurring in GCTBs are derived from tas.},
  author       = {Gebre-Medhin, Samuel and Broberg Palmgren, Karin and Jonson, Tord and Gorunova, Ludmila and Vult von Steyern, Fredrik and Brosjö, O and Jin, Yuesheng and Gisselsson Dahlén, Margareta and Panagopoulos, Ioannis and Mandahl, Nils and Mertens, Fredrik},
  issn         = {1424-859X},
  keyword      = {Telomere-Binding Proteins: metabolism,Telomere-Binding Proteins: genetics,Telomere: metabolism,Telomerase: metabolism,Giant Cell Tumor of Bone: genetics,Telomerase: genetics,Telomeric Repeat Binding Protein 2: genetics,Telomeric Repeat Binding Protein 2: metabolism},
  language     = {eng},
  number       = {2},
  pages        = {121--127},
  publisher    = {Karger},
  series       = {Cytogenetic and Genome Research2002-01-01+01:00},
  title        = {Telomeric associations correlate with telomere length reduction and clonal chromosome aberrations in giant cell tumor of bone.},
  url          = {http://dx.doi.org/10.1159/000207516},
  volume       = {124},
  year         = {2009},
}