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Amperometric monitoring of redox activity in living yeast cells: comparison of menadione and menadione sodium bisulfite as electron transfer mediators

Heiskanen, Arto LU ; Yakovleva, Julia LU ; Spegel, Christer LU ; Taboryski, Rafael; Koudelka-Hep, Milena; Emnéus, Jenny LU and Ruzgas, Tautgirdas LU (2004) In Electrochemistry Communications 6(2). p.219-224
Abstract
An amperometric method was applied for real-time monitoring of intracellular redox enzyme activity. Baker’s yeast (Saccharomyces cerevisiae) cells were immobilized on platinum microband electrodes and mediated anodic currents were measured. The currents were observed in the absence and in the presence of glucose as a source of reducing equivalents, NADH and NADPH. 2-Methyl-1,4-naphthoquinone (menadione, vitamin K3) and water soluble 2-methyl-1,4-naphthoquinone sodium bisulfite (menadione sodium bisulfite MSB) were compared as artificial electron acceptors for their ability to transduce internal cellular redox activity into electrode current. It was found that hydrophobic menadione was superior to its water-soluble bisulfite derivative for... (More)
An amperometric method was applied for real-time monitoring of intracellular redox enzyme activity. Baker’s yeast (Saccharomyces cerevisiae) cells were immobilized on platinum microband electrodes and mediated anodic currents were measured. The currents were observed in the absence and in the presence of glucose as a source of reducing equivalents, NADH and NADPH. 2-Methyl-1,4-naphthoquinone (menadione, vitamin K3) and water soluble 2-methyl-1,4-naphthoquinone sodium bisulfite (menadione sodium bisulfite MSB) were compared as artificial electron acceptors for their ability to transduce internal cellular redox activity into electrode current. It was found that hydrophobic menadione was superior to its water-soluble bisulfite derivative for probing whole intact cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amperometric method, Living cells, Real-time, Artificial electron acceptors, NAD(P)H oxidizing enzymes
in
Electrochemistry Communications
volume
6
issue
2
pages
219 - 224
publisher
Elsevier
external identifiers
  • wos:000188507400024
  • scopus:1042290487
ISSN
1388-2481
DOI
10.1016/j.elecom.2003.12.003
language
English
LU publication?
yes
id
668a0f25-cd86-4136-bfaa-3f773ff639e2 (old id 141267)
date added to LUP
2007-06-27 08:45:00
date last changed
2017-11-19 03:29:33
@article{668a0f25-cd86-4136-bfaa-3f773ff639e2,
  abstract     = {An amperometric method was applied for real-time monitoring of intracellular redox enzyme activity. Baker’s yeast (Saccharomyces cerevisiae) cells were immobilized on platinum microband electrodes and mediated anodic currents were measured. The currents were observed in the absence and in the presence of glucose as a source of reducing equivalents, NADH and NADPH. 2-Methyl-1,4-naphthoquinone (menadione, vitamin K3) and water soluble 2-methyl-1,4-naphthoquinone sodium bisulfite (menadione sodium bisulfite MSB) were compared as artificial electron acceptors for their ability to transduce internal cellular redox activity into electrode current. It was found that hydrophobic menadione was superior to its water-soluble bisulfite derivative for probing whole intact cells.},
  author       = {Heiskanen, Arto and Yakovleva, Julia and Spegel, Christer and Taboryski, Rafael and Koudelka-Hep, Milena and Emnéus, Jenny and Ruzgas, Tautgirdas},
  issn         = {1388-2481},
  keyword      = {Amperometric method,Living cells,Real-time,Artificial electron acceptors,NAD(P)H oxidizing enzymes},
  language     = {eng},
  number       = {2},
  pages        = {219--224},
  publisher    = {Elsevier},
  series       = {Electrochemistry Communications},
  title        = {Amperometric monitoring of redox activity in living yeast cells: comparison of menadione and menadione sodium bisulfite as electron transfer mediators},
  url          = {http://dx.doi.org/10.1016/j.elecom.2003.12.003},
  volume       = {6},
  year         = {2004},
}