Amperometric monitoring of redox activity in living yeast cells: comparison of menadione and menadione sodium bisulfite as electron transfer mediators
(2004) In Electrochemistry Communications 6(2). p.219-224- Abstract
- An amperometric method was applied for real-time monitoring of intracellular redox enzyme activity. Baker’s yeast (Saccharomyces cerevisiae) cells were immobilized on platinum microband electrodes and mediated anodic currents were measured. The currents were observed in the absence and in the presence of glucose as a source of reducing equivalents, NADH and NADPH. 2-Methyl-1,4-naphthoquinone (menadione, vitamin K3) and water soluble 2-methyl-1,4-naphthoquinone sodium bisulfite (menadione sodium bisulfite MSB) were compared as artificial electron acceptors for their ability to transduce internal cellular redox activity into electrode current. It was found that hydrophobic menadione was superior to its water-soluble bisulfite derivative for... (More)
- An amperometric method was applied for real-time monitoring of intracellular redox enzyme activity. Baker’s yeast (Saccharomyces cerevisiae) cells were immobilized on platinum microband electrodes and mediated anodic currents were measured. The currents were observed in the absence and in the presence of glucose as a source of reducing equivalents, NADH and NADPH. 2-Methyl-1,4-naphthoquinone (menadione, vitamin K3) and water soluble 2-methyl-1,4-naphthoquinone sodium bisulfite (menadione sodium bisulfite MSB) were compared as artificial electron acceptors for their ability to transduce internal cellular redox activity into electrode current. It was found that hydrophobic menadione was superior to its water-soluble bisulfite derivative for probing whole intact cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/141267
- author
- Heiskanen, Arto LU ; Yakovleva, Julia LU ; Spegel, Christer LU ; Taboryski, Rafael ; Koudelka-Hep, Milena ; Emnéus, Jenny LU and Ruzgas, Tautgirdas LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Amperometric method, Living cells, Real-time, Artificial electron acceptors, NAD(P)H oxidizing enzymes
- in
- Electrochemistry Communications
- volume
- 6
- issue
- 2
- pages
- 219 - 224
- publisher
- Elsevier
- external identifiers
-
- wos:000188507400024
- scopus:1042290487
- ISSN
- 1388-2481
- DOI
- 10.1016/j.elecom.2003.12.003
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004), Muscle biology (013212015)
- id
- 668a0f25-cd86-4136-bfaa-3f773ff639e2 (old id 141267)
- date added to LUP
- 2016-04-01 11:52:40
- date last changed
- 2024-07-02 13:20:56
@article{668a0f25-cd86-4136-bfaa-3f773ff639e2, abstract = {{An amperometric method was applied for real-time monitoring of intracellular redox enzyme activity. Baker’s yeast (Saccharomyces cerevisiae) cells were immobilized on platinum microband electrodes and mediated anodic currents were measured. The currents were observed in the absence and in the presence of glucose as a source of reducing equivalents, NADH and NADPH. 2-Methyl-1,4-naphthoquinone (menadione, vitamin K3) and water soluble 2-methyl-1,4-naphthoquinone sodium bisulfite (menadione sodium bisulfite MSB) were compared as artificial electron acceptors for their ability to transduce internal cellular redox activity into electrode current. It was found that hydrophobic menadione was superior to its water-soluble bisulfite derivative for probing whole intact cells.}}, author = {{Heiskanen, Arto and Yakovleva, Julia and Spegel, Christer and Taboryski, Rafael and Koudelka-Hep, Milena and Emnéus, Jenny and Ruzgas, Tautgirdas}}, issn = {{1388-2481}}, keywords = {{Amperometric method; Living cells; Real-time; Artificial electron acceptors; NAD(P)H oxidizing enzymes}}, language = {{eng}}, number = {{2}}, pages = {{219--224}}, publisher = {{Elsevier}}, series = {{Electrochemistry Communications}}, title = {{Amperometric monitoring of redox activity in living yeast cells: comparison of menadione and menadione sodium bisulfite as electron transfer mediators}}, url = {{http://dx.doi.org/10.1016/j.elecom.2003.12.003}}, doi = {{10.1016/j.elecom.2003.12.003}}, volume = {{6}}, year = {{2004}}, }