Advanced

Immuno-SLM—a combined sample handling and analytical technique

Tudorache, Madalina LU ; Rak, Mariusz; Wieczorek, Piotr P; Jönsson, Jan Åke LU and Emnéus, Jenny LU (2004) In Journal of Immunological Methods 284(1-2). p.107-118
Abstract
Immuno-supported liquid membrane (immuno-SLM) extraction is a new technique that makes use of antibody (Ab)–antigen interactions as the "extraction force" to drive the mass transfer in a selective way. In immuno-SLM, anti-analyte (Ag) Abs are introduced into the acceptor phase of the SLM unit to trap the Ag that passes from the flowing donor through the SLM into the stagnant acceptor. The amount of immuno-extracted analyte (AbAg) is quantified by connecting the immuno-SLM unit on-line with a non-competitive heterogeneous fluorescence flow immunoassay (FFIA) that makes use of a fluorescein-labeled analyte tracer that titrates the residual excess of Ab present in the acceptor. A restricted access (RA) column is used for the separation of the... (More)
Immuno-supported liquid membrane (immuno-SLM) extraction is a new technique that makes use of antibody (Ab)–antigen interactions as the "extraction force" to drive the mass transfer in a selective way. In immuno-SLM, anti-analyte (Ag) Abs are introduced into the acceptor phase of the SLM unit to trap the Ag that passes from the flowing donor through the SLM into the stagnant acceptor. The amount of immuno-extracted analyte (AbAg) is quantified by connecting the immuno-SLM unit on-line with a non-competitive heterogeneous fluorescence flow immunoassay (FFIA) that makes use of a fluorescein-labeled analyte tracer that titrates the residual excess of Ab present in the acceptor. A restricted access (RA) column is used for the separation of the two tracer fractions (Ag* and AbAg*) formed, and the eluted AbAg* fraction is measured downstream by a fluorescence detector.



Factors influencing the optimum immuno-SLM extraction parameters, i.e., donor flow rate, extraction time and type of Ab, were investigated for immuno extraction of the model analyte atrazine. Immuno-SLM coupled to FFIA (immuno-SLM–FFIA) and FFIA alone were compared in terms of the assay sensitivities obtained and the sample matrix influence. The concentration at the mid-point of the calibration curve (IC50) was 16.0±1.4 and 36±16 g/l, the limit of detection (LOD) was 2.0±1.1 and 20±10 g/l, and the dynamic range was 2–100 and 20–500 g/l atrazine for immuno-SLM–FFIA and FFIA, respectively. The matrix influence on the FFIA was significant in orange juice and surface water, whereas the influence was minor for immuno-SLM–FFIA with recoveries between 104% and 115% for 5 g/l atrazine in tap water, orange juice and river water. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
orange juice, river water, tap water, atrazine, restricted access, fluorescein, supported liquid membrane extraction, immuno extraction, immunoassay
in
Journal of Immunological Methods
volume
284
issue
1-2
pages
107 - 118
publisher
Elsevier
external identifiers
  • pmid:14736421
  • wos:000188598400011
  • scopus:0346373831
ISSN
1872-7905
DOI
10.1016/j.jim.2003.10.014
language
English
LU publication?
yes
id
9f82ac8c-10b6-4ded-a950-a7e11314ddd9 (old id 141305)
date added to LUP
2007-06-28 09:25:00
date last changed
2017-01-01 06:46:13
@article{9f82ac8c-10b6-4ded-a950-a7e11314ddd9,
  abstract     = {Immuno-supported liquid membrane (immuno-SLM) extraction is a new technique that makes use of antibody (Ab)–antigen interactions as the "extraction force" to drive the mass transfer in a selective way. In immuno-SLM, anti-analyte (Ag) Abs are introduced into the acceptor phase of the SLM unit to trap the Ag that passes from the flowing donor through the SLM into the stagnant acceptor. The amount of immuno-extracted analyte (AbAg) is quantified by connecting the immuno-SLM unit on-line with a non-competitive heterogeneous fluorescence flow immunoassay (FFIA) that makes use of a fluorescein-labeled analyte tracer that titrates the residual excess of Ab present in the acceptor. A restricted access (RA) column is used for the separation of the two tracer fractions (Ag* and AbAg*) formed, and the eluted AbAg* fraction is measured downstream by a fluorescence detector.<br/><br>
<br/><br>
Factors influencing the optimum immuno-SLM extraction parameters, i.e., donor flow rate, extraction time and type of Ab, were investigated for immuno extraction of the model analyte atrazine. Immuno-SLM coupled to FFIA (immuno-SLM–FFIA) and FFIA alone were compared in terms of the assay sensitivities obtained and the sample matrix influence. The concentration at the mid-point of the calibration curve (IC50) was 16.0±1.4 and 36±16 g/l, the limit of detection (LOD) was 2.0±1.1 and 20±10 g/l, and the dynamic range was 2–100 and 20–500 g/l atrazine for immuno-SLM–FFIA and FFIA, respectively. The matrix influence on the FFIA was significant in orange juice and surface water, whereas the influence was minor for immuno-SLM–FFIA with recoveries between 104% and 115% for 5 g/l atrazine in tap water, orange juice and river water.},
  author       = {Tudorache, Madalina and Rak, Mariusz and Wieczorek, Piotr P and Jönsson, Jan Åke and Emnéus, Jenny},
  issn         = {1872-7905},
  keyword      = {orange juice,river water,tap water,atrazine,restricted access,fluorescein,supported liquid membrane extraction,immuno extraction,immunoassay},
  language     = {eng},
  number       = {1-2},
  pages        = {107--118},
  publisher    = {Elsevier},
  series       = {Journal of Immunological Methods},
  title        = {Immuno-SLM—a combined sample handling and analytical technique},
  url          = {http://dx.doi.org/10.1016/j.jim.2003.10.014},
  volume       = {284},
  year         = {2004},
}