Advanced

Diagnostic PCR: validation and sample preparation are two sides of the same coin

Hoorfar, Jeffrey; Wolffs, Petra LU and Rådström, Peter LU (2004) In APMIS : acta pathologica, microbiologica, et immunologica Scandinavica1988-01-01+01:00 112(11-12). p.808-814
Abstract
Increased use of powerful PCR technology for the routine detection of pathogens has focused attention on the need for international validation and preparation of official non-commercial guidelines. Bacteria of epidemiological importance should be the prime focus, although a "validation infrastructure" once established could easily be adapted for PCR-based detection of viruses and parasites. The aim of standardization should be the widespread adoption of diagnostic PCR for routine pathogen testing. European experience provides the impetus for realization of this vision through preparation of quantitative reference DNA material and reagents, production of stringent protocols and tools for thermal cycler performance testing, uncomplicated... (More)
Increased use of powerful PCR technology for the routine detection of pathogens has focused attention on the need for international validation and preparation of official non-commercial guidelines. Bacteria of epidemiological importance should be the prime focus, although a "validation infrastructure" once established could easily be adapted for PCR-based detection of viruses and parasites. The aim of standardization should be the widespread adoption of diagnostic PCR for routine pathogen testing. European experience provides the impetus for realization of this vision through preparation of quantitative reference DNA material and reagents, production of stringent protocols and tools for thermal cycler performance testing, uncomplicated sample preparation techniques, and extensive ring trials for assessment of the efficacy of selected matrix/pathogen detection protocols. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica1988-01-01+01:00
volume
112
issue
11-12
pages
808 - 814
publisher
John Wiley & Sons
external identifiers
  • wos:000226305700007
  • scopus:12744272142
ISSN
1600-0463
DOI
10.1111/j.1600-0463.2004.apm11211-1207.x
language
English
LU publication?
yes
id
0f853823-0d22-485b-93b7-820a8b0d5779 (old id 141750)
date added to LUP
2007-06-28 13:31:46
date last changed
2017-07-30 03:42:15
@article{0f853823-0d22-485b-93b7-820a8b0d5779,
  abstract     = {Increased use of powerful PCR technology for the routine detection of pathogens has focused attention on the need for international validation and preparation of official non-commercial guidelines. Bacteria of epidemiological importance should be the prime focus, although a "validation infrastructure" once established could easily be adapted for PCR-based detection of viruses and parasites. The aim of standardization should be the widespread adoption of diagnostic PCR for routine pathogen testing. European experience provides the impetus for realization of this vision through preparation of quantitative reference DNA material and reagents, production of stringent protocols and tools for thermal cycler performance testing, uncomplicated sample preparation techniques, and extensive ring trials for assessment of the efficacy of selected matrix/pathogen detection protocols.},
  author       = {Hoorfar, Jeffrey and Wolffs, Petra and Rådström, Peter},
  issn         = {1600-0463},
  language     = {eng},
  number       = {11-12},
  pages        = {808--814},
  publisher    = {John Wiley & Sons},
  series       = { APMIS : acta pathologica, microbiologica, et immunologica Scandinavica1988-01-01+01:00},
  title        = {Diagnostic PCR: validation and sample preparation are two sides of the same coin},
  url          = {http://dx.doi.org/10.1111/j.1600-0463.2004.apm11211-1207.x},
  volume       = {112},
  year         = {2004},
}