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In vivo compartmentalization of functionally distinct, rapidly responsive antigen-specific T-cell populations in DNA-immunized or Salmonella enterica serovar Typhimurium-infected mice

Kirby, Alun C.; Sundquist, Malin and Wick, Mary Jo LU (2004) In Infection and Immunity 72(11). p.6390-6400
Abstract
The location and functional properties of antigen-specific memory T-cell populations in lymphoid and nonlymphoid compartments following DNA immunization or infection with Salmonella were investigated. Epitope-specific CD8+-T-cell expansion and retention during the memory phase were analyzed for DNA-immunized mice by use of a 5-h peptide restimulation assay. These data revealed that epitope-specific gamma interferon (IFN-{gamma})-positive CD8+ T cells occur at higher frequencies in the spleen, liver, and blood than in draining or peripheral lymph nodes during the expansion phase. Moreover, this distribution is maintained into long-term memory. The location and function of both CD4+ and CD8+ Salmonella-specific memory T cells in mice who... (More)
The location and functional properties of antigen-specific memory T-cell populations in lymphoid and nonlymphoid compartments following DNA immunization or infection with Salmonella were investigated. Epitope-specific CD8+-T-cell expansion and retention during the memory phase were analyzed for DNA-immunized mice by use of a 5-h peptide restimulation assay. These data revealed that epitope-specific gamma interferon (IFN-{gamma})-positive CD8+ T cells occur at higher frequencies in the spleen, liver, and blood than in draining or peripheral lymph nodes during the expansion phase. Moreover, this distribution is maintained into long-term memory. The location and function of both CD4+ and CD8+ Salmonella-specific memory T cells in mice who were given a single dose of Salmonella enterica serovar Typhimurium was also quantitated by an ex vivo restimulation with bacterial lysate to detect the total Salmonella-specific memory pool. Mice immunized up to 6 months previously with S. enterica serovar Typhimurium had bacterium-specific CD4+ T cells that were capable of producing IFN-{gamma} or tumor necrosis factor alpha (TNF-{alpha}) at each site analyzed. Similar findings were observed for CD8+ T cells that were capable of producing IFN-{gamma}, while a much lower frequency and more restricted distribution were associated with TNF-{alpha}-producing CD8+ T cells. This study is the first to assess the frequencies, locations, and functions of both CD4+ and CD8+ memory T-cell populations in the same Salmonella-infected individuals and demonstrates the organ-specific functional compartmentalization of memory T cells after Salmonella infection. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Infection and Immunity
volume
72
issue
11
pages
6390 - 6400
publisher
American Society for Microbiology
external identifiers
  • wos:000224664300027
  • pmid:15501769
  • scopus:7044254923
ISSN
1098-5522
DOI
10.1128/IAI.72.11.6390-6400.2004
language
English
LU publication?
yes
id
e791f9d8-92d8-44b6-ba92-212b174ebddf (old id 141831)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15501769&query_hl=45
date added to LUP
2007-07-16 12:36:32
date last changed
2017-02-12 03:40:22
@article{e791f9d8-92d8-44b6-ba92-212b174ebddf,
  abstract     = {The location and functional properties of antigen-specific memory T-cell populations in lymphoid and nonlymphoid compartments following DNA immunization or infection with Salmonella were investigated. Epitope-specific CD8+-T-cell expansion and retention during the memory phase were analyzed for DNA-immunized mice by use of a 5-h peptide restimulation assay. These data revealed that epitope-specific gamma interferon (IFN-{gamma})-positive CD8+ T cells occur at higher frequencies in the spleen, liver, and blood than in draining or peripheral lymph nodes during the expansion phase. Moreover, this distribution is maintained into long-term memory. The location and function of both CD4+ and CD8+ Salmonella-specific memory T cells in mice who were given a single dose of Salmonella enterica serovar Typhimurium was also quantitated by an ex vivo restimulation with bacterial lysate to detect the total Salmonella-specific memory pool. Mice immunized up to 6 months previously with S. enterica serovar Typhimurium had bacterium-specific CD4+ T cells that were capable of producing IFN-{gamma} or tumor necrosis factor alpha (TNF-{alpha}) at each site analyzed. Similar findings were observed for CD8+ T cells that were capable of producing IFN-{gamma}, while a much lower frequency and more restricted distribution were associated with TNF-{alpha}-producing CD8+ T cells. This study is the first to assess the frequencies, locations, and functions of both CD4+ and CD8+ memory T-cell populations in the same Salmonella-infected individuals and demonstrates the organ-specific functional compartmentalization of memory T cells after Salmonella infection.},
  author       = {Kirby, Alun C. and Sundquist, Malin and Wick, Mary Jo},
  issn         = {1098-5522},
  language     = {eng},
  number       = {11},
  pages        = {6390--6400},
  publisher    = {American Society for Microbiology},
  series       = {Infection and Immunity},
  title        = {In vivo compartmentalization of functionally distinct, rapidly responsive antigen-specific T-cell populations in DNA-immunized or Salmonella enterica serovar Typhimurium-infected mice},
  url          = {http://dx.doi.org/10.1128/IAI.72.11.6390-6400.2004},
  volume       = {72},
  year         = {2004},
}