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Ultrastructural cartilage abnormalities in MIA/CD-RAP-deficient mice

Moser, Markus; Bosserhoff, Anja-Katrin; Hunziker, Ernst B.; Sandell, Linda; Fässler, Reinhard LU and Buettner, Reinhard (2002) In Molecular and Cellular Biology 22(5). p.1438-1445
Abstract
MIA/CD-RAP is a small, soluble protein secreted from malignant melanoma cells and from chondrocytes. Recent evidence has identified MIA/CD-RAP as the prototype of a small family of extracellular proteins adopting an SH3 domain-like fold. It is thought that interaction between MIA/CD-RAP and specific epitopes in extracellular matrix proteins regulates the attachment of tumor cells and chondrocytes. In order to study the consequences of MIA/CD-RAP deficiency in vivo, we generated mice with a targeted gene disruption. The complete absence of MIA/CD-RAP mRNA and protein expression was demonstrated by reverse transcriptase, Western blot analysis, and enzyme-linked immunosorbent assay measurements of whole-embryo extracts. MIA-/- mice were... (More)
MIA/CD-RAP is a small, soluble protein secreted from malignant melanoma cells and from chondrocytes. Recent evidence has identified MIA/CD-RAP as the prototype of a small family of extracellular proteins adopting an SH3 domain-like fold. It is thought that interaction between MIA/CD-RAP and specific epitopes in extracellular matrix proteins regulates the attachment of tumor cells and chondrocytes. In order to study the consequences of MIA/CD-RAP deficiency in vivo, we generated mice with a targeted gene disruption. The complete absence of MIA/CD-RAP mRNA and protein expression was demonstrated by reverse transcriptase, Western blot analysis, and enzyme-linked immunosorbent assay measurements of whole-embryo extracts. MIA-/- mice were viable and developed normally, and histological examination of the organs by means of light microscopy revealed no major abnormalities. In contrast, electron microscopic studies of cartilage composition revealed subtle defects in collagen fiber density, diameter, and arrangement, as well as changes in the number and morphology of chondrocytic microvilli. Taken together, our data indicate that MIA/CD-RAP is essentially required for formation of the highly ordered ultrastructural fiber architecture in cartilage and may have a role in regulating chondrocyte matrix interactions. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular and Cellular Biology
volume
22
issue
5
pages
1438 - 1445
publisher
American Society for Microbiology
external identifiers
  • pmid:11839810
  • wos:000173837000016
  • scopus:0036172080
ISSN
0270-7306
DOI
10.1128/MCB.22.5.1438-1445.2002
language
English
LU publication?
yes
id
1eec37b8-4125-49dc-930f-86e86c3dcf08 (old id 141957)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11839810&query_hl=132
date added to LUP
2007-07-19 15:44:52
date last changed
2017-09-24 03:27:10
@article{1eec37b8-4125-49dc-930f-86e86c3dcf08,
  abstract     = {MIA/CD-RAP is a small, soluble protein secreted from malignant melanoma cells and from chondrocytes. Recent evidence has identified MIA/CD-RAP as the prototype of a small family of extracellular proteins adopting an SH3 domain-like fold. It is thought that interaction between MIA/CD-RAP and specific epitopes in extracellular matrix proteins regulates the attachment of tumor cells and chondrocytes. In order to study the consequences of MIA/CD-RAP deficiency in vivo, we generated mice with a targeted gene disruption. The complete absence of MIA/CD-RAP mRNA and protein expression was demonstrated by reverse transcriptase, Western blot analysis, and enzyme-linked immunosorbent assay measurements of whole-embryo extracts. MIA-/- mice were viable and developed normally, and histological examination of the organs by means of light microscopy revealed no major abnormalities. In contrast, electron microscopic studies of cartilage composition revealed subtle defects in collagen fiber density, diameter, and arrangement, as well as changes in the number and morphology of chondrocytic microvilli. Taken together, our data indicate that MIA/CD-RAP is essentially required for formation of the highly ordered ultrastructural fiber architecture in cartilage and may have a role in regulating chondrocyte matrix interactions.},
  author       = {Moser, Markus and Bosserhoff, Anja-Katrin and Hunziker, Ernst B. and Sandell, Linda and Fässler, Reinhard and Buettner, Reinhard},
  issn         = {0270-7306},
  language     = {eng},
  number       = {5},
  pages        = {1438--1445},
  publisher    = {American Society for Microbiology},
  series       = {Molecular and Cellular Biology},
  title        = {Ultrastructural cartilage abnormalities in MIA/CD-RAP-deficient mice},
  url          = {http://dx.doi.org/10.1128/MCB.22.5.1438-1445.2002},
  volume       = {22},
  year         = {2002},
}