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Vascular effects of anandamide and N-acylvanillylamines in the human forearm and skin microcirculation.

Movahed Rad, Pouya LU ; Evilevitch, Vladimir LU ; Andersson, Tomas LU ; Jönsson, Bo A LU ; Wollmer, Per LU ; Zygmunt, Peter LU and Högestätt, Edward LU (2005) In British Journal of Pharmacology 146(2). p.171-179
Abstract
1 The endocannabinoid anandamide is an emerging potential signalling molecule in the cardiovascular system. Anandamide causes vasodilatation, bradycardia and hypotension in animals and has been implicated in the pathophysiology of endotoxic, haemorrhagic and cardiogenic shock, but its vascular effects have not been studied in man. 2 Human forearm blood flow and skin microcirculatory flow were recorded using venous occlusion plethysmography and laser-Doppler perfusion imaging ( LDPI), respectively. Each test drug was infused into the brachial artery or applied topically on the skin followed by a standardized pin-prick to disrupt the epidermal barrier. 3 Anandamide failed to affect forearm blood flow when administered intra-arterially at... (More)
1 The endocannabinoid anandamide is an emerging potential signalling molecule in the cardiovascular system. Anandamide causes vasodilatation, bradycardia and hypotension in animals and has been implicated in the pathophysiology of endotoxic, haemorrhagic and cardiogenic shock, but its vascular effects have not been studied in man. 2 Human forearm blood flow and skin microcirculatory flow were recorded using venous occlusion plethysmography and laser-Doppler perfusion imaging ( LDPI), respectively. Each test drug was infused into the brachial artery or applied topically on the skin followed by a standardized pin-prick to disrupt the epidermal barrier. 3 Anandamide failed to affect forearm blood flow when administered intra-arterially at infusion rates of 0.3-300 nmol min(-1). The highest infusion rate led to an anandamide concentration of approximately 1 mu M in venous blood as measured by mass spectrometry. 4 Dermal application of anandamide significantly increased skin microcirculatory flow and coapplication of the transient receptor potential vanilloid 1 ( TRPV1) antagonist capsazepine inhibited this effect. The TRPV1 agonists capsaicin, olvanil and arvanil all induced concentration-dependent increases in skin blood flow and burning pain when administered dermally. Coapplication of capsazepine inhibited blood flow and pain responses to all three TRPV1 agonists. 5 This study shows that locally applied anandamide is a vasodilator in the human skin microcirculation. The results are consistent with this lipid being an activator of TRPV1 on primary sensory nerves, but do not support a role for anandamide as a circulating vasoactive hormone in the human forearm vascular bed. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
anandamide, human skin, lipid messengers, calcium channels, vasodilatation
in
British Journal of Pharmacology
volume
146
issue
2
pages
171 - 179
publisher
The British Pharmacological Society
external identifiers
  • pmid:15997233
  • wos:000232317100003
  • scopus:30544440893
ISSN
1476-5381
DOI
10.1038/sj.bjp.0706313
language
English
LU publication?
yes
id
146b1beb-2c74-4742-b2c4-22637b9471e5 (old id 142362)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15997233&dopt=Abstract
date added to LUP
2007-07-05 14:22:15
date last changed
2017-08-13 04:13:45
@article{146b1beb-2c74-4742-b2c4-22637b9471e5,
  abstract     = {1 The endocannabinoid anandamide is an emerging potential signalling molecule in the cardiovascular system. Anandamide causes vasodilatation, bradycardia and hypotension in animals and has been implicated in the pathophysiology of endotoxic, haemorrhagic and cardiogenic shock, but its vascular effects have not been studied in man. 2 Human forearm blood flow and skin microcirculatory flow were recorded using venous occlusion plethysmography and laser-Doppler perfusion imaging ( LDPI), respectively. Each test drug was infused into the brachial artery or applied topically on the skin followed by a standardized pin-prick to disrupt the epidermal barrier. 3 Anandamide failed to affect forearm blood flow when administered intra-arterially at infusion rates of 0.3-300 nmol min(-1). The highest infusion rate led to an anandamide concentration of approximately 1 mu M in venous blood as measured by mass spectrometry. 4 Dermal application of anandamide significantly increased skin microcirculatory flow and coapplication of the transient receptor potential vanilloid 1 ( TRPV1) antagonist capsazepine inhibited this effect. The TRPV1 agonists capsaicin, olvanil and arvanil all induced concentration-dependent increases in skin blood flow and burning pain when administered dermally. Coapplication of capsazepine inhibited blood flow and pain responses to all three TRPV1 agonists. 5 This study shows that locally applied anandamide is a vasodilator in the human skin microcirculation. The results are consistent with this lipid being an activator of TRPV1 on primary sensory nerves, but do not support a role for anandamide as a circulating vasoactive hormone in the human forearm vascular bed.},
  author       = {Movahed Rad, Pouya and Evilevitch, Vladimir and Andersson, Tomas and Jönsson, Bo A and Wollmer, Per and Zygmunt, Peter and Högestätt, Edward},
  issn         = {1476-5381},
  keyword      = {anandamide,human skin,lipid messengers,calcium channels,vasodilatation},
  language     = {eng},
  number       = {2},
  pages        = {171--179},
  publisher    = {The British Pharmacological Society},
  series       = {British Journal of Pharmacology},
  title        = {Vascular effects of anandamide and N-acylvanillylamines in the human forearm and skin microcirculation.},
  url          = {http://dx.doi.org/10.1038/sj.bjp.0706313},
  volume       = {146},
  year         = {2005},
}