Reappraisal of the intravenous glucose tolerance index for a simple assessment of insulin sensitivity in mice
(2009) In American Journal of Physiology: Regulatory, Integrative and Comparative Physiology 296(5). p.13161324 Abstract
 Pacini G, Ahren M, Ahren B. Reappraisal of the intravenous glucose tolerance index for a simple assessment of insulin sensitivity in mice. Am J Physiol Regul Integr Comp Physiol 296: R1316R1324, 2009. First published February 11, 2009; doi: 10.1152/ajpregu.90575.2008. Mice are increasingly used in studies where measuring insulin sensitivity (IS) is a common procedure. The glucose clamp is labor intensive, cannot be used in large numbers of animals, cannot be repeated in the same mouse, and has been questioned as a valid tool for IS in mice; thus, the minimal model with 50min intravenous glucose tolerance test (IVGTT) data was adapted for studies in mice. However, specific software and particular ability was needed. The aim of this study... (More)
 Pacini G, Ahren M, Ahren B. Reappraisal of the intravenous glucose tolerance index for a simple assessment of insulin sensitivity in mice. Am J Physiol Regul Integr Comp Physiol 296: R1316R1324, 2009. First published February 11, 2009; doi: 10.1152/ajpregu.90575.2008. Mice are increasingly used in studies where measuring insulin sensitivity (IS) is a common procedure. The glucose clamp is labor intensive, cannot be used in large numbers of animals, cannot be repeated in the same mouse, and has been questioned as a valid tool for IS in mice; thus, the minimal model with 50min intravenous glucose tolerance test (IVGTT) data was adapted for studies in mice. However, specific software and particular ability was needed. The aim of this study was to establish a simple procedure for evaluating IS during IVGTT in mice (CSI). IVGTTs (n = 520) were performed in NMRI and C57BL/6J mice (2025g). After glucose injection (1 g/kg), seven samples were collected for 50 min for glucose and insulin measurements, analyzed with a minimal model that provided the validated reference IS (Sperpendicular to). By using the regression CS perpendicular to = alpha(1) + alpha(2) x KG/AUC(D), where KG is intravenous glucose tolerance index and AUC(D) is the dynamic area under the curve, IS was calculated in 134 control animals randomly selected (regression CSI vs. SI: r = 0.66, P < 0.0001) and yielded alpha(1) = 1.93 and alpha(2) = 0.24. KG is the slope of log (glucose(520)) and AUCD is the mean dynamic area under insulin curve in the IVGTT. By keeping fixed alpha(1) and alpha(2), CSI was validated in 143 control mice (4.7 +/ 0.2 min . mu U . ml(1), virtually identical to SI: 4.7 +/ 0.3, r = 0.89, P < 0.0001); and in 123 mice in different conditions: transgenic, addition of neuropeptides, incretins, and insulin (CSI: 6.0 +/ 0.4 vs. SI: 6.1 +/ 0.4, r = 0.94, P < 0.0001). In the other 120 animals, CSI revealed its ability to segregate different categories, as does SI. This easily usable formula for calculating CSI overcomes many experimental obstacles and may be a simple alternative to more complex procedures when large numbers of mice or repeated experiments in the same animals are required. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1425759
 author
 Pacini, Giovanni ; Ahren, Maria and Ahrén, Bo ^{LU}
 organization
 publishing date
 2009
 type
 Contribution to journal
 publication status
 published
 subject
 keywords
 intravenous glucose tolerance test, glucose effectiveness, adenylate cyclaseactivating polypeptide, pituitary, glucagonlike peptide1, insulin resistance, animal model
 in
 American Journal of Physiology: Regulatory, Integrative and Comparative Physiology
 volume
 296
 issue
 5
 pages
 1316  1324
 publisher
 American Physiological Society
 external identifiers

 wos:000266246900003
 scopus:66149096190
 pmid:19211728
 ISSN
 03636119
 DOI
 10.1152/ajpregu.90575.2008
 language
 English
 LU publication?
 yes
 id
 98914c6d16d34ba39c1f6d150402b322 (old id 1425759)
 date added to LUP
 20160401 14:04:18
 date last changed
 20210901 04:31:49
@article{98914c6d16d34ba39c1f6d150402b322, abstract = {Pacini G, Ahren M, Ahren B. Reappraisal of the intravenous glucose tolerance index for a simple assessment of insulin sensitivity in mice. Am J Physiol Regul Integr Comp Physiol 296: R1316R1324, 2009. First published February 11, 2009; doi: 10.1152/ajpregu.90575.2008. Mice are increasingly used in studies where measuring insulin sensitivity (IS) is a common procedure. The glucose clamp is labor intensive, cannot be used in large numbers of animals, cannot be repeated in the same mouse, and has been questioned as a valid tool for IS in mice; thus, the minimal model with 50min intravenous glucose tolerance test (IVGTT) data was adapted for studies in mice. However, specific software and particular ability was needed. The aim of this study was to establish a simple procedure for evaluating IS during IVGTT in mice (CSI). IVGTTs (n = 520) were performed in NMRI and C57BL/6J mice (2025g). After glucose injection (1 g/kg), seven samples were collected for 50 min for glucose and insulin measurements, analyzed with a minimal model that provided the validated reference IS (Sperpendicular to). By using the regression CS perpendicular to = alpha(1) + alpha(2) x KG/AUC(D), where KG is intravenous glucose tolerance index and AUC(D) is the dynamic area under the curve, IS was calculated in 134 control animals randomly selected (regression CSI vs. SI: r = 0.66, P < 0.0001) and yielded alpha(1) = 1.93 and alpha(2) = 0.24. KG is the slope of log (glucose(520)) and AUCD is the mean dynamic area under insulin curve in the IVGTT. By keeping fixed alpha(1) and alpha(2), CSI was validated in 143 control mice (4.7 +/ 0.2 min . mu U . ml(1), virtually identical to SI: 4.7 +/ 0.3, r = 0.89, P < 0.0001); and in 123 mice in different conditions: transgenic, addition of neuropeptides, incretins, and insulin (CSI: 6.0 +/ 0.4 vs. SI: 6.1 +/ 0.4, r = 0.94, P < 0.0001). In the other 120 animals, CSI revealed its ability to segregate different categories, as does SI. This easily usable formula for calculating CSI overcomes many experimental obstacles and may be a simple alternative to more complex procedures when large numbers of mice or repeated experiments in the same animals are required.}, author = {Pacini, Giovanni and Ahren, Maria and Ahrén, Bo}, issn = {03636119}, language = {eng}, number = {5}, pages = {13161324}, publisher = {American Physiological Society}, series = {American Journal of Physiology: Regulatory, Integrative and Comparative Physiology}, title = {Reappraisal of the intravenous glucose tolerance index for a simple assessment of insulin sensitivity in mice}, url = {http://dx.doi.org/10.1152/ajpregu.90575.2008}, doi = {10.1152/ajpregu.90575.2008}, volume = {296}, year = {2009}, }