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Delayed nerve repair increases number of caspase 3 stained Schwann cells

Saito, Harukazu; Kanje, Martin LU and Dahlin, Lars LU (2009) In Neuroscience Letters 456(1). p.30-33
Abstract
Caspase 3 staining in Schwann cells was investigated with immunohistochemistry, as a measure of Schwann cell apoptosis, after transection and immediate (day 0) or delayed rat sciatic nerve repair (30, 90 and 180 days post injury). Cleaved caspase 3 stained Schwann cells significantly increased at the site of lesion (SNL; median [IQR], 15.2 [7.0] %) and in the distal nerve segment (SND; 9.5 [3.6] %) 10 days after immediate repair. The number of cleaved caspase stained Schwann cells also increased significantly after delayed repair, irrespective of length of delay, at both locations (SNL: 22.0-27.1%; SND: 18.5-22.1%; p < 0.05). Some cleaved caspase 3 stained satellite cells were seen in dorsal root ganglia on the injured side, but no... (More)
Caspase 3 staining in Schwann cells was investigated with immunohistochemistry, as a measure of Schwann cell apoptosis, after transection and immediate (day 0) or delayed rat sciatic nerve repair (30, 90 and 180 days post injury). Cleaved caspase 3 stained Schwann cells significantly increased at the site of lesion (SNL; median [IQR], 15.2 [7.0] %) and in the distal nerve segment (SND; 9.5 [3.6] %) 10 days after immediate repair. The number of cleaved caspase stained Schwann cells also increased significantly after delayed repair, irrespective of length of delay, at both locations (SNL: 22.0-27.1%; SND: 18.5-22.1%; p < 0.05). Some cleaved caspase 3 stained satellite cells were seen in dorsal root ganglia on the injured side, but no stained motor or sensory neurons were observed at any time-point. Delayed nerve repair is associated with more pronounced Schwann cell apoptosis which may explain impaired nerve regeneration after nerve injury and delayed repair. (C) 2009 Elsevier Ireland Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cells, Satellite, Nerve repair, Apoptosis, Schwann cells, Cleaved caspase 3
in
Neuroscience Letters
volume
456
issue
1
pages
30 - 33
publisher
Elsevier
external identifiers
  • wos:000266062500007
  • scopus:64749100267
ISSN
0304-3940
DOI
10.1016/j.neulet.2009.03.075
language
English
LU publication?
yes
id
0aa6a551-35f3-4659-8a9c-3005884d346c (old id 1425860)
date added to LUP
2009-06-26 14:55:42
date last changed
2017-10-22 03:42:18
@article{0aa6a551-35f3-4659-8a9c-3005884d346c,
  abstract     = {Caspase 3 staining in Schwann cells was investigated with immunohistochemistry, as a measure of Schwann cell apoptosis, after transection and immediate (day 0) or delayed rat sciatic nerve repair (30, 90 and 180 days post injury). Cleaved caspase 3 stained Schwann cells significantly increased at the site of lesion (SNL; median [IQR], 15.2 [7.0] %) and in the distal nerve segment (SND; 9.5 [3.6] %) 10 days after immediate repair. The number of cleaved caspase stained Schwann cells also increased significantly after delayed repair, irrespective of length of delay, at both locations (SNL: 22.0-27.1%; SND: 18.5-22.1%; p &lt; 0.05). Some cleaved caspase 3 stained satellite cells were seen in dorsal root ganglia on the injured side, but no stained motor or sensory neurons were observed at any time-point. Delayed nerve repair is associated with more pronounced Schwann cell apoptosis which may explain impaired nerve regeneration after nerve injury and delayed repair. (C) 2009 Elsevier Ireland Ltd. All rights reserved.},
  author       = {Saito, Harukazu and Kanje, Martin and Dahlin, Lars},
  issn         = {0304-3940},
  keyword      = {cells,Satellite,Nerve repair,Apoptosis,Schwann cells,Cleaved caspase 3},
  language     = {eng},
  number       = {1},
  pages        = {30--33},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {Delayed nerve repair increases number of caspase 3 stained Schwann cells},
  url          = {http://dx.doi.org/10.1016/j.neulet.2009.03.075},
  volume       = {456},
  year         = {2009},
}